scholarly journals Fluvo-metricell, a combined cell volume and cell fluorescence analyzer.

1977 ◽  
Vol 25 (7) ◽  
pp. 804-812 ◽  
Author(s):  
V Kachel ◽  
E Glossner ◽  
E Kordwig ◽  
G Ruhenstroth-Bauer

A new flow through instrument that simultaneously measures cell volume (resistance pulse technique) and cell fluorescence in the same orifice will be described. The fluorescence pulses of the hydrodynamically focussed cells are picked up by the optics via the axial direction (principle of Dittrich and Goehde, Z Naturforsch 24b:360, 1969). There is no coordination problem between the fluorescence and the resistance pulses to be observed because a new type of transducer is used. The electronic system provides gating of one or two parameter histograms. Function tests are performed with the incorporated two-parameter test spectrum generator. Different examples of using the instrument in practice are shown. The volume that may be measured with an orifice of 70 micron diameter ranges between 4 and 1400 micron3 (1:350). Coefficients of variation of the fluorescence below 2% are measured.

2020 ◽  
Author(s):  
Vahid Shahabadi ◽  
Daryoush Abdollahpour

Abstract Superposition of two circular Airy vortex beams (CAVB), with opposite sign topological charges (l), produces a new type of petal beams called petal-like circular Airy beam (PCAB) with a transverse field distribution in the form of azimuthally modulated concentric rings that follow Airy function over the radial distance on a transverse plane. In this paper, tight focusing of truncated PCAB and its application in optical trapping is numerically investigated. It is shown that by adjusting the beam parameters four different trapping configurations can be achieved: a single transverse trap at a single axial position, a multi-trap geometry at a single axial position, two single transverse traps at two positions along the axial direction, and two multi-trap geometries at two different axial positions. It is also shown that the number of trapped particles in the multi-trap configurations is 2l per focal plane, while the number of axial trap positions is determined by the truncation aperture size. Finally, trap stiffnesses and corresponding potential energies for the trapping configurations are presented and discussed.


2018 ◽  
Vol 22 (2) ◽  
pp. 76-85
Author(s):  
Rama Shanker ◽  
Kamlesh Kumar Shukla

A zero-truncated discrete two-parameter Poisson-Lindley distribution (ZTDTPPLD), which includes zero-truncated Poisson-Lindley distribution (ZTPLD) as a particular case, has been introduced. The proposed distribution has been obtained by compounding size-biased Poisson distribution (SBPD) with a continuous distribution. Its raw moments and central moments have been given. The coefficients of variation, skewness, kurtosis, and index of dispersion have been obtained and their nature and behavior have been studied graphically. Maximum likelihood estimation (MLE) has been discussed for estimating its parameters. The goodness of fit of ZTDTPPLD has been discussed with some data sets and the fit shows satisfactory over zero – truncated Poisson distribution (ZTPD) and ZTPLD. Journal of Institute of Science and TechnologyVolume 22, Issue 2, January 2018, Page: 76-85


2017 ◽  
Author(s):  
Christopher R. Chartier

Tacit coordination is a fundamental task for interdependent social actors. Everyday examples include negotiating rush hour traffic, working collaboratively on a group project, conversing smoothly, and meeting friends for lunch. Previous research (Schelling, 1960; Mehta, Starmer & Sugden, 1994) has focused extensively on differences between behavioral options that aid in small group coordination. Highly salient options are referred to as structural focal points, and are popular choices for actors. The current work presents a new type of focal point based on differences between social actors in a coordination problem. The focal social actor effect is hypothesized to occur when one group member is more salient than others, and actors correspond to behavioral options in a clear way. Three Experiments explored and ultimately supported this hypothesis. In Experiment 1, social information about group members was manipulated such that one group member was unique. Furthermore, players were identified by color. In a subsequent coordination game, players were more likely to select an option sharing an identifying color with the unique group member than an option corresponding to a common player. In Experiment 2, actors and options corresponded through slight payoff differences between group members across different behavioral options. Once again, players were more likely to select the option corresponding to the unique player. In Experiment 3, uniqueness was manipulated orthogonally to intragroup status by virtue of bogus feedback on a leadership questionnaire. The focal social actor served as a coordination cue, as players were more likely to select the option corresponding to the unique player. The focal social actor cue was found to be effective even in the presence of the previously established status cue for coordination (de Kwaadsteniet & van Dijk, 2010). Focal social actors serve as strong coordination aids for small groups, and may help explain portions of previously published tacit coordination findings.


2011 ◽  
Vol 183-185 ◽  
pp. 1913-1917
Author(s):  
Gui Fan Zhao ◽  
Zi Peng Zhang ◽  
Tso Liang Teng

In this paper, an experimental model was built in the nonlinear finite element software LS-DYNA. A metal tube wrapped fiberglass epoxy composite outside (called “composite tube” for short) was impacted in its axial direction in the simulation. The key problems about building the model were discussed later. Comparing the simulation and experiment results, we proved the validity of the model. Furthermore, we applied the material type of the composite tube to vehicle longeron, and carried on the simulation of automobile frontal collisions in LS-DYNA. The simulation results showed using the new type composite material could reduce the longeron weight to original 33.76% without lowering the safety.


1981 ◽  
Vol 27 (7) ◽  
pp. 1287-1290 ◽  
Author(s):  
A Ohkubo ◽  
S Kamei ◽  
M Yamanaka ◽  
F Arai ◽  
M Kitajima ◽  
...  

Abstract A new type of multilayer film analytical element--consisting of a spreading layer, a blocking layer, an enzymic reagent layer, and a transparent layer--has been developed for blood glucose determination. After a spot of whole blood is dropped on the film, the plasma glucose concentration is determined in 6 min without further manipulations. Precise measurement of sample volume spotted on the film is not necessary for good results, nor is knowledge of the hematocrit in the range 10-46%. The minimum concentration of glucose detectable by this method is 100 mg/L. The coefficients of variation, within-run and between-run, were all less than 2.5% for 800-4000 mg/L glucose concentrations. Macromolecules and hydrophobic substances in blood did not interfere. This method seems suitable for emergency use, especially.


1988 ◽  
Vol 37 (1-2) ◽  
pp. 29-40 ◽  
Author(s):  
T. S. K. Moothathu

Here we develop the uniformly minimum variance unbiased (best) estimators of coefficient of variation, Pearson's coefficients of skewness and kurtosis of two-parameter Pareto distribution, the variance of these best estimators and the best estimators of those variances. The best estimators are in terms of Kummer's function 1 F1 and Humbert's function ø2 ; and their variances are in terms of F2 , the Appel function of second kind and K 12 , a quadruple hypergeornetric function invented by Exton during the early 1970's.


1987 ◽  
Vol 109 (4) ◽  
pp. 359-366 ◽  
Author(s):  
Y. Yoshinaga ◽  
T. Kaneki ◽  
H. Kobayashi ◽  
M. Hoshino

The experimental results from eighteen different centrifugal compressor stages showed that the pressure recovery of vaneless diffusers for high specific speed compressors was extremely low compared with the value expected by an ideal two-dimensional analysis. Consequently a new type of diffuser with half guide vanes on the shroud side wall was proposed. The pressure recovery of this diffuser at distorted inlet flow was considerably improved by the half guide vanes. The optimum height of the vanes was a little less than one half of the diffuser width. Measurements of the velocity distribution in the diffusers using Pitot tubes and a laser-two-focus velocimeter, clearly showed that the small height guide vanes gave a uniform flow in the axial direction and improved the pressure recovery of the diffuser.


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