scholarly journals The histochemical demonstration of polycytidylic acid and polyuridylic acid hydrolases in rat liver during azo dye carcinogenesis.

1977 ◽  
Vol 25 (6) ◽  
pp. 458-465
Author(s):  
R Daoust
Keyword(s):  
Rat Liver ◽  
Azo Dye ◽  
Histochemical Demonstration ◽  
Acid Hydrolases ◽  
Polycytidylic Acid ◽  
Polyuridylic Acid

Effects of Polynucleotides on Production and Activity of Streptolysin S

1980 ◽  
Vol 35 (7-8) ◽  
pp. 578-584 ◽  
Author(s):  
Yoriko Taketo ◽  
Akira Taketo
Keyword(s):  
Rat Liver ◽  
Deae Cellulose ◽  
Inhibitory Effect ◽  
Cell System ◽  
Streptolysin S ◽  
Carrier Effect ◽  
Polycytidylic Acid ◽  
Polyuridylic Acid ◽  
Potent Inhibitory Effect ◽  
Bacterial Culture Supernatant

Activity of various natural and synthetic polynucleotides as a carrier for streptolysin S was tested in a resting cell system. As the carrier, intact molecules of MS2 RNA, E. coli tRNA or rat liver RNA were almost inactive, whereas RNase I core of these RNAs, especially the core-frac­tions eluted from DEAE-cellulose column at higher NaCl concentrations, effectively induced pro­duction of the extracellular hemolysin. The carrier activity of yeast RNA was significantly en­hanced by simultaneous addition of RNase I, to the streptococcal suspension. Preincubation of yeast RNA with growing streptococci or a protein fraction from the bacterial culture supernatant increased its carrier activity. Evidences were obtained suggesting involvement of streptococcal nuclease in the enhancement of the carrier effect. Production of the streptococcal hemolysin was markedly promoted by polyguanylic acid but neither by polyadenylic acid, polycytidylic acid nor by polyuridylic acid. Like trypan blue, polyguanylic acid exerted potent inhibitory effect on hemolytic activity of streptolysin S complex. Similar but less marked effect was observed with certain RNase I core fractions of rat liver RNA. Some data concerning effect of polynucleotides on stability of the hemolysin were also presented.

scholarly journals The histochemical demonstration of hydrolase activities with films of homopolyribonucleotides.

1975 ◽  
Vol 23 (1) ◽  
pp. 51-58 ◽  
Author(s):  
R Daoust
Keyword(s):  
Ribonucleic Acid ◽  
Histochemical Demonstration ◽  
Tissue Sections ◽  
Polycytidylic Acid ◽  
Polyadenylic Acid ◽  
Polyuridylic Acid

Films of polycytidylic acid, polyuridylic acid and polyguanylic acid were exposed to tissue sections and the results were compared with those obtained in previous studies on polyadenylic acid and ribonucleic acid. Important variations were observed in the distribution of the hydrolases acting on the different polyribonucleotides, suggesting that a variety of nucleases with marked proclivity for particular nucleotide residues can be demonstrated by the use of films of homopolymers.

scholarly journals Polyribonucleotide synthesis by subfractions of microsomes from rat liver

1968 ◽  
Vol 109 (2) ◽  
pp. 229-238 ◽  
Author(s):  
N. M. Wilkie ◽  
R. M. S. Smellie
Keyword(s):  
Acetic Acid ◽  
Rat Liver ◽  
Transfer Rna ◽  
Light Fraction ◽  
Free Ribosome ◽  
Polyadenylic Acid ◽  
Insoluble Material ◽  
Polyuridylic Acid ◽  
Microsome Fraction

1. The microsome fraction of rat liver has been fractionated and the ability of the fractions to incorporate ribonucleotides into polyribonucleotides has been studied. Activity was found in the rough-surfaced vesicle (light) fraction and in the free-ribosome fraction and this latter activity has been examined. 2. The free-ribosome fraction contains ribosome monomers, dimers and trimers together with some higher oligomers and ferritin. In addition to catalysing the incorporation of ribonucleotides into acid-insoluble material it contains diesterase activity. It catalyses the incorporation of UMP from UTP, but not UDP, AMP from ATP and CMP from CTP into polyribonucleotide material, and for UTP the product appears to be a homopolymer not more than eight units long attached to the ends of primer polyribonucleotide strands. 3. The activity could not be removed from the free-ribosome fraction by washing or by isolation in the presence of ethylenediaminetetra-acetic acid. 4. Partially hydrolysed polyuridylic acid but not polyadenylic acid could serve as a primer for the incorporation of UMP, but some activity was always associated with an endogenous primer. 5. Analysis of RNA extracted from the free-ribosome fraction after incubation with [3H]UTP showed the presence of 28s, 18s, 5s and transfer RNA types, but no radioactivity was associated with any of these RNA fractions.

Changes of aldolase A and B messenger RNA levels in rat liver during azo-dye-induced hepatocarcinogenesis

1984 ◽  
Vol 124 (2) ◽  
pp. 337-343 ◽  
Author(s):  
Makoto Daimon ◽  
Ken-ichi Tsutsumi ◽  
Jun-ichi Sato ◽  
Reiko Tsutsumi ◽  
Kiichi Ishikawa
Keyword(s):  
Rat Liver ◽  
Messenger Rna ◽  
Azo Dye ◽  
Aldolase A ◽  
Rna Levels

scholarly journals Effect of azo-dye carcinogenesis on enzymes concerned with urea synthesis in rat liver

1964 ◽  
Vol 91 (3) ◽  
pp. 464-473 ◽  
Author(s):  
P McLean ◽  
E Reid ◽  
MW Gurney
Keyword(s):  
Rat Liver ◽  
Azo Dye ◽  
Urea Synthesis

Differential ricin sensitivity of rat liver and wheat germ ribosomes in polyuridylic acid translation

1977 ◽  
Vol 182 (2) ◽  
pp. 690-695 ◽  
Author(s):  
Daniel B. Cawley ◽  
Mary L. Hedblom ◽  
Eugene J. Hoffman ◽  
L.L. Houston
Keyword(s):  
Rat Liver ◽  
Wheat Germ ◽  
Polyuridylic Acid
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