scholarly journals Further evidence in support of cell-surface-associated deoxyribonucleic acid in tumor cells: an autoradiographic study.

1977 ◽  
Vol 25 (5) ◽  
pp. 359-370 ◽  
Author(s):  
S K Aggarwal
Keyword(s):  
Plasma Membrane ◽  
Deoxyribonucleic Acid ◽  
Tritiated Thymidine ◽  
Membrane Surface ◽  
Autoradiographic Study ◽  
Sarcoma 180 ◽  
Grain Distribution ◽  
Tumor Implant ◽  
Plasma Membrane Surface

Ascites sarcoma-180 cells, when stained with platinum-pyrimidine complexes as the sole electron dense stain, show distinct dense patches to granular appearance on the surface of the plasma membrane which has been suggested to be attributable to deoxyribonucleic acid. Swiss Webster mice, 4-5 weeks of age, weighing 24-26 g with 4 X 10(6) ascites sarcoma-180 cells when injected with 3 X 7.0 micronC of tritiated thymidine on day 5 of the tumor implant, show specific labeling on the plasma membrane surface. The photopositive silver grain distribution in both the light and electron microscope autoradiograms when followed from the nucleus outwards show a distinct peak over the nucleus and the plasma membrane. The quantity and origin and role of this surface-associated deoxyribonucleic acid is not clear.

Visualization of Surface-Associated Nucleic Acid in Tumoricenic Cells Using Ultrathin Frozen Sections and Platinum-Pyrimidine Complexes

1976 ◽  
Vol 34 ◽  
pp. 204-205
Author(s):  
Surinder K. Aggarwal
Keyword(s):  
Mammalian Cells ◽  
Tritiated Thymidine ◽  
Membrane Surface ◽  
Sarcoma 180 ◽  
Frozen Sections ◽  
Tumor Biopsy ◽  
Normal Cells ◽  
Ultrathin Frozen Sections ◽  
Tumor Implant ◽  
Plasma Membrane Surface

Platinum-pyrimidine complexes have been used as sole electron-dense stains for electron microscopy on number of mammalian cells (1-3) . They are selective for areas rich in nucleic acids and stain the cellular chromatin, ribosomes and nucleolus. Tumorigenic cells exhibit special binding on the plasma membrane surface in the form of dense patches; normal cells do not. Solid tumor biopsy samples from animals and humans show similar patches but only in malignant tissues. Various enzymatic studies suggest that the electron-dense patches are DNA with neuraminic acid involved in the binding. Autoradiographic studies using H3 -thymidine confirms the presence of surface- associated DNA.Swiss Webster mice bearing ascites sarcoma-180 tumor are given intraperitoneal injection of 7. 0 μ c of tritiated thymidine in 0. 5 ml sterile 0. 85% NaCI on day 5 of the tumor implant.

Role of plasma membrane surface charges in dictating the feasibility of membrane-nanoparticle interactions

2017 ◽  
Vol 111 (26) ◽  
pp. 263702 ◽  
Author(s):  
Shayandev Sinha ◽  
Haoyuan Jing ◽  
Harnoor Singh Sachar ◽  
Siddhartha Das
Keyword(s):  
Plasma Membrane ◽  
Membrane Surface ◽  
Surface Charges ◽  
Nanoparticle Interactions ◽  
Plasma Membrane Surface

scholarly journals Paracrystalline arrays of membrane-to-membrane cross bridges associated with the inner surface of plasma membrane

1978 ◽  
Vol 77 (2) ◽  
pp. 323-328 ◽  
Author(s):  
WW Franke ◽  
C Grund ◽  
E Schmid ◽  
E Mandelkow
Keyword(s):  
Plasma Membrane ◽  
Plasma Membranes ◽  
Cultured Cells ◽  
Membrane Surface ◽  
Hexagonal Lattice ◽  
Interparticle Distance ◽  
Macromolecular Complexes ◽  
Inner Surface ◽  
Cross Bridges ◽  
Plasma Membrane Surface

In cultured cells of the rat kangaroo PtK2 line, veils of the cell surface were observed which consisted of only plasma membrane and paracrystalline arrays of membrane-associated particles sandwiched in between. These membrane-to-membrane cross-bridging 9-to 11-nm wide particles were somewhat coumellar-shaped and were arranged on a hexagonal lattice with an interparticle distance of 16nm. At higher magnification, they revealed an unstained core, thus suggesting a ringlike substructure. Similar arrays of paracrystal-containing veils, which were rather variable in size and frequency, were also observed in other cultured cells. It is hypothesized that these paracrystals represent protein macromolecular complexes associated with the inner plasma membrane surface which crystallize when plasma membranes come into close intracellular contact and other components of the subsurface network are removed.

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