scholarly journals Immunocytochemical localization of a folicle stimulating hormone-like molecule in the testis.

1977 ◽  
Vol 25 (10) ◽  
pp. 1119-1126 ◽  
Author(s):  
J C Hutson ◽  
P J Gardner ◽  
G C Moriarty
Keyword(s):  
Germ Cells ◽  
Follicle Stimulating Hormone ◽  
Staining Intensity ◽  
The Body ◽  
Nuclear Staining ◽  
Adult Rat ◽  
Ultrathin Sections ◽  
Immunocytochemical Studies ◽  
Unlabeled Antibody ◽  
Stimulating Hormone

A follicle-stimulating hormone (FSH)-like molecule was localized in normal adult rat testes as well as testosterone-treated hypophysectomized rat tests with an unlabeled antibody (anti-FSH), peroxidase-antiperoxidase complex technique. Anti-FSH bound specifically to ultrathin sections of acrosomes of spermatids and intranuclear bodies of early spermatids. Quantitation of staining intensity demonstrated that FSH, used as an absorbing antigen, would significantly reduce this binding. There was less anti-FSH binding to the acrosomes of spermatozoa in the body and tail of the epididymis as compared to the less mature germ cells located in the testis and head of the epididymis. The acrosomal and nuclear staining of spermatids taken from hypophysectomized animals was similar to staining observed in sham injected animals. Taken together, these results suggest that there is a molecule within the acrosome that is immunologically similar to FSH. Most importantly, these results emphasize the importance of conducting physiologic experiments in conjunction with immunocytochemical studies.

scholarly journals ELECTRON MICROSCOPIC STUDY OF THE ADRENOCORTICOTROPIN-PRODUCING CELL WITH THE USE OF UNLABELED ANTIBODY AND THE SOLUBLE PEROXIDASE-ANTIPEROXIDASE COMPLEX

1972 ◽  
Vol 20 (8) ◽  
pp. 590-603 ◽  
Author(s):  
G. C. MORIARTY ◽  
N. S. HALMI
Keyword(s):  
Secretory Granules ◽  
Staining Intensity ◽  
Electron Microscopic ◽  
Early Effect ◽  
Acth Cell ◽  
Maximal Diameter ◽  
Soluble Peroxidase ◽  
Ultrathin Sections ◽  
Acth Secreting ◽  
Unlabeled Antibody

The technique involving use of unlabeled antibody and the peroxidase-antiperoxidase complex was used to identify the adrenocorticotropin (ACTH)-secreting cell in the anterior pituitary lobe of the rat and to localize ACTH in it electron microscopically in ultrathin sections. The ACTH cell is star-shaped, with processes extending around other cells, and contains secretory granules of a maximal diameter of 300 mµ arranged peripherally along the plasma membrane. Stain was observed on secretory granules, around them, in the Golgi complex and in rough endoplasmic reticulum. One day after adrenalectomy, the ACTH cell is degranulated and the staining intensity of its remaining granules and cytoplasm is decreased, suggesting release of ACTH stores. If cortisol is given 6 hr after adrenalectomy, 18 hr later the ACTH cells are well granulated and the granules stain more intensely than normal. In addition, staining around the granules and throughout the cytoplasm is more intense, suggesting that an early effect of cortisol is to block release of ACTH. Twenty-one days after adrenalectomy, the ACTH cells are greatly increased in numbers and have complex, tortuous processes filled with intensely stained secretory granules.

MT1-MMP in rat testicular development and the control of Sertoli cell proMMP-2 activation

2001 ◽  
Vol 114 (11) ◽  
pp. 2125-2134 ◽  
Author(s):  
Juliette Longin ◽  
Patricia Guillaumot ◽  
Marie-Agnès Chauvin ◽  
Anne-Marie Morera ◽  
Brigitte Le Magueresse-Battistoni
Keyword(s):  
Germ Cells ◽  
Follicle Stimulating Hormone ◽  
Developmental Period ◽  
Testicular Development ◽  
Hormone Regulation ◽  
Cell Lineages ◽  
Apical Compartment ◽  
Membrane Type ◽  
Stimulating Hormone

Metalloproteases (MMPs) are likely to be involved in the restructuring events occurring in the testis throughout development. We here demonstrate that membrane-type 1 (MT1)-MMP, a physiological activator of proMMP-2 under TIMP-2 control, is present within the testis together with MMP-2 and TIMP-2. In the prepubertal testis MT1-MMP immunoreactivity was uniformly distributed, whereas in the adult it was confined to the apical compartment of the tubules, where meiosis and spermiogenesis occur. We further showed that the two cell lineages (somatic and germinal) expressed MT1-MMP and TIMP-2, whereas MMP-2 was of somatic origin. To get a better picture into proMMP-2 activation, use was made of a model of cultured Sertoli cells treated with FSH or co-cultured with germ cells to mimic an immature or a mature developmental period, respectively. We found that follicle-stimulating hormone enhanced the expression of MMP-2 and TIMP-2 but not of MT1-MMP, and promoted the activation of proMMP-2. In co-cultures, a tremendous elevation and activation of MMP-2 was observed, which might relate to the processed MT1-MMP form solely detected in germ cells. That MMP-2 synthesis and activation are under local (germ cells) and hormonal (follicle-stimulating hormone) regulation emphasizes the importance of MMPs in testicular physiology.

236. Effects of long term recombinant rat follicle-stimulating hormone replacement on the restoration of spermatogenesis after chronic suppression of gonadotrophins in adult rats

2008 ◽  
Vol 20 (9) ◽  
pp. 36
Author(s):  
S. M. Ruwanpura ◽  
P. G. Stanton ◽  
D. M. Robertson ◽  
R. I. McLachlan ◽  
Y. Makanji ◽  
...  
Keyword(s):  
Sertoli Cell ◽  
Germ Cells ◽  
Follicle Stimulating Hormone ◽  
Inhibin B ◽  
Testis Weight ◽  
Adult Rats ◽  
Early Pachytene ◽  
Pachytene Spermatocytes ◽  
Stimulating Hormone

Follicle stimulating hormone (FSH) in short-term rat studies supports spermatogenesis at multiple levels, notably spermatogonial development. The role of FSH in supporting full spermatogenesis in rats is still in question as long-term studies have not been possible due the development of neutralising antibodies to heterologous FSH preparations. This study sought to assess the effects of a homologous recombinant rat FSH (rr-FSH) preparation on the long-term restoration of spermatogenesis. Adult rats were GnRH-immunised (GnRH-im) for 12 weeks then, administered an anti-androgen; flutamide (flut), alone or together with rr-FSH (8µg/rat/daily) for 56 days (1 spermatogenic cycle). Germ and Sertoli cell numbers were quantified using an optical disector stereological method. Testis weight, serum FSH and inhibin B and Sertoli cell nuclear volume were significantly reduced to 15%, 13%, 25% and 57% of controls respectively, following GnRH-im+flut treatment. GnRH-im+flut treatment reduced A/I spermatogonial, type B spermatogonial+preleptotene, leptotene+zygotene and early pachytene spermatocyte numbers to 28%, 68%, 50% and 19% (P < 0.001) of controls respectively, with later germ cells rarely observed. After FSH treatment, no significant affect on testis weight, serum FSH and inhibin B or Sertoli cell number were observed. However, rr-FSH treatment significantly increased numbers of A/I spermatogonia, leptotene+zygotene and early pachytene spermatocytes from 28 = >42%, 50 = >69% and 19 = >27% of controls, respectively, while no differences were observed in later germ cell types. rr-FSH also increased (P < 0.05) the volume of Sertoli cell nuclei from 57 = >66% of control. In conclusion, FSH is unable to support full rat spermatogenesis; however, FSH can partially support germ cells notably spermatogonia through to early pachytene spermatocytes, despite the absence of androgenic support.

scholarly journals Anti-androgenic Impact of Nauclea latifolia on Testicular Weight, Testosterone, Follicle Stimulating Hormone and Glycogen Granules of the Testes

2021 ◽  
Vol 3 (4) ◽  
pp. 6-10
Author(s):  
Eno-Obong I. Bassey ◽  
Gabriel D. Edem ◽  
Kingsley A. Okon ◽  
Aquaisua N. Aquaisua
Keyword(s):  
Leaf Extract ◽  
Periodic Acid ◽  
Follicle Stimulating Hormone ◽  
The Body ◽  
Testicular Weight ◽  
The Impact ◽  
Ethanolic Leaf Extract ◽  
Hormonal Assay ◽  
Nauclea Latifolia ◽  
Stimulating Hormone

Purpose: Over time, plants have been a major source of medicine to treat and cure various ailments and diseases. In many cases, the dosages and standardization of these herbal products have been so ignored that it results in these products causing damages to certain organs of the body unnoticed. The impact of ethanolic leaf extract of Nauclea latifolia on the testes was determined using Wistar rats. Method: 500, 1000 and 1500 mg of ethanolic leaf extract of Nauclea latifolia /kg body weight was administered for 21 days to 4 groups of animals (n=10). Blood sample was taken for hormonal assay, testicular weight measured, and histological procedure done, and the tissues stained with PAS. Results: Result from hormonal assay showed reduced level of testosterone and follicle stimulating hormone. After histological staining using periodic acid Schiff method (PAS) and subsequent examination using a compound microscope, there was a reduced level of glycogen granules in all the Nauclea latifolia groups. Findings from this study also showed that the ethanolic leaf extract of Nauclea latifolia at all doses significantly reduced testicular weight (p<0.05). Conclusion: This study reveals that Nauclea latifolia is anti-androgenic.

scholarly journals PRESTASI BERANAK TERNAK BABI YANG MENGGUNAKAN HORMON PMSG DAN hCG PADA PETERNAKAN KOMERSIAL DI KELURAHAN KAYAWU

ZOOTEC ◽  
2013 ◽  
Vol 33 (1) ◽  
pp. 58
Author(s):  
Tery Wenda ◽  
Frans A. Kairupan ◽  
Petrus R. R. I. Montong ◽  
Sjaloom E. Sakul ◽  
Mien Th. R. Lapian
Keyword(s):  
Birth Weight ◽  
Litter Size ◽  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Follicle Stimulating Hormone ◽  
The Body ◽  
Average Birth Weight ◽  
Randomized Design ◽  
Serum Gonadotropin ◽  
Stimulating Hormone

ABSTRAKSuperovulasi merupakan salah satu upaya untuk meningkatkan derajat ovulasi dengan penyuntikan hormon gonadotropin pada tubuh hewan betina. Pada umumnya hewan donor disuntik dengan preparat follicle stimulating hormone (FSH) danPregnant mare's serum gonadotropin(PMSG) atau kombinasi (Pregnant mare's serum gonadotropin) dan human chorionic gonadotropin (hCG).Tujuan Penelitian iniadalah untuk mengetahui sejauh mana prestasi beranak babi dara (calon induk) dengan menggunakan hormon pregnant mare's serum gonadropin (PMSG) dan human Chorionic Gonadotropin (hCG).Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) terdiri atas dua perlakuan (menggunakan dan tanpa menggunakan hormon PMSG dan hCG) dengan masing-masing 21 ulangan. Semua data dianalisa dengan analisis sidik ragam (ANOVA) Steel dan Torrie (1993).Variabel yang diamati untuk adalah litter size, berat lahir anak babi yang dilahirkan dan mortalitas anak babi yang lahir sampai disapih. Hasil penelitian menunjukkan bahwa jumlah anak babi yang lahir hidup dalam satu kelahiran ada perbedaan sangat nyata bahwa yang lahir dari induk Superovulasi dibandingkan dengan induk yang tidak disuperovulasikan, rata-rata 9.19-6.66 ekor. Dan juga berat lahir rata-rata 1.53-1.40 kg Demikian juga mortalitas anak rata-rata 14.36-29.19%. Hasil penelitian menunjukkan bahwa penggunakan hormon PMSG dan hCG dapat memperbaiki litter size lahir, bobot lahir, dan menekan persentase mortalitas ternak babi. Kata Kunci:Potensi beranak babi dara,hormon PMSG dan hCG, Babi betina bunting.ABSTRACTTHE ACHIEVEMENT PIGS BEAR LIVESTOCK USING PMSG AND hCG HORMONE ON RANCH IN THE VILLAGE COMMERCIAL KAYAWU. Superovulation is an effort to increase the degree of ovulation by injection of gonadotropin hormones in the body of a female animal. In general, animal donors were injected with preparations of follicle stimulating hormone (FSH) and Pregnant mare's serum gonadotropin (PMSG) or a combination (Pregnant mare's serum gonadotropin) and human chorionic gonadotropin (hCG). The objective of this research was to determine the extent of achievement farrow dara (prospective parent) using pregnant mare's serum hormone gonadropin (PMSG) and human chorionic gonadotropin (hCG). The research using completely randomized design (CRD) consisting of two treatments (using and without using PMSG and hCG hormone) with 21 replications each. All data were analyzed with analysis of variance (ANOVA) Steel and Torrie (1993). Variables was observed for litter size, birth weight piglets born and piglets born mortality until weaning. The results showed that the number of piglets born alive in the birth there is a very real difference that is born of a parent superovulation compared with the parent who does not to superovulated on average 9.19–6.66 tail. And also the average birth weight 1:53 to 1:40 kilograms likewise the average child mortality 14.36–29.19%. From the results of the study concluded that the use of PMSG and hCG hormone can improve birth litter size, birth weight and reduce the percentage of mortality of pigs. Keywords: Extent of achievement of gilt,PMSG dan hCG hormone, and Pregnant mare.

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