scholarly journals Pancreatic islet cell hormones distribution of cell types in the islet and evidence for the presence of somatostatin and gastrin within the D cell.

1976 ◽  
Vol 24 (7) ◽  
pp. 883-897 ◽  
Author(s):  
S L Erlandsen ◽  
O D Hegre ◽  
J A Parsons ◽  
R C McEvoy ◽  
R P Elde
Keyword(s):  
Pancreatic Islet ◽  
Islet Cell ◽  
Cell Types ◽  
Pancreatic Islet Cell ◽  
D Cell ◽  
Islet Cell Hormones

An experimental investigation into the possible origin of pancreatic islet cells from rhombencephalic neurectoderm

Development ◽  
1979 ◽  
Vol 52 (1) ◽  
pp. 23-38
Author(s):  
Ann Andrew ◽  
Beverley Kramer
Keyword(s):  
Pancreatic Islet ◽  
Islet Cell ◽  
Islet Cells ◽  
Cell Types ◽  
Nuclear Marker ◽  
Electron Microscopic ◽  
Pancreatic Islet Cell ◽  
Enteric Ganglia ◽  
Microscopic Features ◽  
Islet Cell Types

To determine whether or not any pancreatic islet cell type arises from rhombencephalic levels of neurectoderm, lengths of presumptive rhombencephalon (containing potential neural crest) of Black Australorp chick embryos at 6- to 9-somite stages were replaced isotopically and isochronically by neural tube of Japanese quail embryos. Some transplants included mesencephalic regions. In some cases various levels of the rhombencephalon were deleted and not replaced. The quail nuclear marker was detected in cranial ganglia in operated embryos sacrificed at 3¾ days of incubation and in enteric ganglia and cells accompanying some pancreatic nerves, in embryos killed at 7 days of incubation. This provided evidence of normal migration of crest cells from the grafts. Dopa was administered to the younger embryos, which were submitted to the formaldehyde-induced fluorescence procedure to demonstrate APUD (Amine Precursor Uptake and Decarboxylation) cells. No pancreatic APUD cells exhibited the quail nuclear marker. In 9- to 11-day embryos, A and B cells were identified by specific light and electron microscopic features. None showed the quail marker. The marker was also absent from those D cells seen and from cells of an as yet unidentified type, but not enough of these were found to warrant a conclusion. All islet cell types were found in embryos from which various levels of the rhombencephalon had been deleted. It is concluded that at least A and B islet cells are not derived from the rhombencephalic neurectoderm and probably not from mesencephalic levels. Their most likely origin remains the endoderm, which was the accepted source until recently

scholarly journals Single‐cell transcriptomes reveal characteristic features of human pancreatic islet cell types

EMBO Reports ◽  
2015 ◽  
Vol 17 (2) ◽  
pp. 178-187 ◽  
Author(s):  
Jin Li ◽  
Johanna Klughammer ◽  
Matthias Farlik ◽  
Thomas Penz ◽  
Andreas Spittler ◽  
...  
Keyword(s):  
Single Cell ◽  
Pancreatic Islet ◽  
Islet Cell ◽  
Cell Types ◽  
Pancreatic Islet Cell ◽  
Human Pancreatic Islet ◽  
Characteristic Features ◽  
Islet Cell Types

Distribution and density of melatonin receptors in human main pancreatic islet cell types

2018 ◽  
Vol 65 (1) ◽  
pp. e12480 ◽  
Author(s):  
Juliane Zibolka ◽  
Ivonne Bazwinsky-Wutschke ◽  
Eckhard Mühlbauer ◽  
Elmar Peschke
Keyword(s):  
Pancreatic Islet ◽  
Islet Cell ◽  
Cell Types ◽  
Melatonin Receptors ◽  
Pancreatic Islet Cell ◽  
Islet Cell Types

Silver Staining of Pancreatic Islets as Revealed by Electron Microscopy

1967 ◽  
Vol 25 ◽  
pp. 44-45
Author(s):  
Kazuaki Misugi ◽  
Nobuko Misugi ◽  
Hiroshi Yamada
Keyword(s):  
Pancreatic Islet ◽  
Silver Staining ◽  
Islet Cells ◽  
Severe Hypoglycemia ◽  
Granular Cell ◽  
Electron Microscopic Level ◽  
Staining Reaction ◽  
Electron Microscopic ◽  
Pancreatic Islet Cell ◽  
D Cell

The authors had described the fine structure of a type of pancreatic islet cell, which appeared different from typical alpha and beta cells, and tentatively considered that this third type of granular cell probably represents the D cell (Figure 1).Since silver staining has been widely used to differentiate different types of pancreatic islet cells by light microscopy, an attempt to examine this staining reaction at the electron microscopic level was made.Material and Method: Surgically removed specimens from three infants who suffered from severe hypoglycemia were used. The specimens were fixed and preserved in 20% neutral formalin. Frozen sections, 30 to 40 micron thick, were prepared and they were stained by Bielschowsky's method as modified by Suzuki (2). The stained sections were examined under a microscope and islet tissues were isolated. They were fixed in 1% osmium tetroxide in phosphate buffer for one hour and embedded in Epon 812 following dehydration through a series of alcohols and propylene oxide.

Islet-on-a-chip: Biomimetic micropillar-based microfluidic system for three-dimensional pancreatic islet cell culture

2021 ◽  
Vol 183 ◽  
pp. 113215
Author(s):  
Patrycja Sokolowska ◽  
Kamil Zukowski ◽  
Justyna Janikiewicz ◽  
Elzbieta Jastrzebska ◽  
Agnieszka Dobrzyn ◽  
...  
Keyword(s):  
Cell Culture ◽  
Pancreatic Islet ◽  
Islet Cell ◽  
Three Dimensional ◽  
Microfluidic System ◽  
Pancreatic Islet Cell ◽  
Islet Cell Culture
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