scholarly journals Histochemical characteristics of the striated inclusions of adrenoleukodystrophy.

1976 ◽  
Vol 24 (6) ◽  
pp. 725-730 ◽  
Author(s):  
A B Johnson ◽  
H H Schaumburg ◽  
J M Powers
Keyword(s):  
Periodic Acid ◽  
Brain Macrophages ◽  
Cortical Cells ◽  
Periodic Acid Schiff ◽  
Nonpolar Solvents ◽  
Pale Pink ◽  
Biochemical Identification ◽  
Adrenal Cortical Cells ◽  
Cryostat Sections ◽  
Oil Red O

The straited accumulations in adrenal cortical cells and brain macrophages that are characteristic of adrenoleukodystrophy have been studied histochemically in cryostat sections to seek leads for the biochemical identification of the striated material. It stained pale pink with oil red O and did not stain with the Schultz cholesterol procedure or periodic acid-Schiff technique. By utilizing the birefringence of the accumulations as a marker, it was determined that, unlike natural cholesterol and cholesterol esters, the striated material was resistant to acetone and ethanol extraction. It was readily soluble, however, in nonpolar solvents such as n-hexane and chloroform. These findings indicated that the material was most probably a lipid, and they suggested that sequential extraction of adrenoleukodystrophy adrenal and brain with acetone and then n-hexane could be used to isolate this material in relatively pure form. Based on this lead, biochemical studies have just revealed a fatty acid abnormality in adrenoleukodystrophy which appears to be unique to this genetic disease.

Pulmonary Hyalinosis in Dogs

1976 ◽  
Vol 13 (2) ◽  
pp. 138-142 ◽  
Author(s):  
G. E. Dagle ◽  
R. E. Filipy ◽  
R. R. Adee ◽  
B. O. Stuart
Keyword(s):  
Storage Disease ◽  
Periodic Acid ◽  
Alveolar Cells ◽  
Periodic Acid Schiff ◽  
Uranium Ore ◽  
High Incidence ◽  
Radon Daughters ◽  
Ultrastructural Appearance ◽  
Oil Red O

Pulmonary hyalinosis occurred in Beagles exposed to radon daughters with uranium ore dust. The lesion was composed of alveolar cells distended with material positive for periodic acid-Schiff (PAS) and oil red O that ultrastructurally consisted of a whorled arrangement of lamellar membranes suggestive of a storage disease. The high incidence in exposed dogs and the ultrastructural appearance suggested the material originated endogenously as a degenerative response to injury.

scholarly journals The Cytochemistry of Acute Leukemia: Observations on Glycogen and Neutral Fat in Bone Marrow Aspirates

Blood ◽  
1969 ◽  
Vol 33 (2) ◽  
pp. 341-347 ◽  
Author(s):  
JOHN M. BENNETT ◽  
THOMAS F. DUTCHER
Keyword(s):  
Bone Marrow ◽  
Acute Leukemia ◽  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Cytochemical Study ◽  
Blast Cells ◽  
Schiff Reaction ◽  
Granulocytic Leukemia ◽  
Oil Red O

Abstract A cytochemical study of 54 cases of acute leukemia utilizing the periodic acid-Schiff reaction for glycogen and Oil Red O for neutral fat was performed on air dried bone marrow smears. The majority of leukemic lymphoblasts revealed significant amounts of glycogen and neutral fat. "Blast" cells in granulocytic leukemia were devoid of glycogen and of neutral fat. The recommendation is made that both of these histochemical stains be employed to attempt to resolve the problem of specific cellular identification in acute leukemia.

scholarly journals Effect of short-term exercise training on intramyocellular lipid content

2012 ◽  
Vol 37 (5) ◽  
pp. 822-828 ◽  
Author(s):  
Sudip Bajpeyi ◽  
Melissa A. Reed ◽  
Sara Molskness ◽  
Christopher Newton ◽  
Charles J. Tanner ◽  
...  
Keyword(s):  
Exercise Training ◽  
Muscle Glycogen ◽  
Periodic Acid ◽  
Intramyocellular Lipid ◽  
Periodic Acid Schiff ◽  
Before And After ◽  
Intramyocellular Lipid Content ◽  
Type 2 Diabetic ◽  
Oil Red O

The purpose of this study was to investigate the influence of exercise training on intramyocellular lipid (IMCL) content and test the hypothesis that the effect of endurance-oriented exercise training on IMCL is dependent on characteristics of the population studied. Lean (N = 11, body mass index (BMI) = 22.2 ± 0.7 kg·m–2), obese (N = 14, BMI = 38.8 ± 1.7 kg·m–2), and type 2 diabetic (N = 9, BMI = 35.5 ± 2.5 kg·m–2) participants were examined before and after 10 consecutive days of endurance-oriented (60 min·day–1 at ~70% [Formula: see text]O2peak) exercise training. IMCL and muscle glycogen were measured by Oil-Red-O and periodic acid – Schiff staining, respectively. The results indicated that IMCL was elevated (p < 0.05) in the obese and diabetic groups compared with the lean subjects prior to training. After training, IMCL content decreased (–35%) in the participants with type 2 diabetes; there were no changes in IMCL in the lean or obese groups. Muscle glycogen content was lower in the diabetic subjects than in the lean subjects both before and after training. These data indicate that changes in IMCL with exercise training do not exhibit a universal response but rather depend on the metabolic status of the population studied.

scholarly journals Pulmonary hyalinosis in captive sugar gliders (Petaurus breviceps)

2017 ◽  
Vol 29 (5) ◽  
pp. 691-695 ◽  
Author(s):  
Set A. Sokol ◽  
Dalen W. Agnew ◽  
Anne D. Lewis ◽  
Teresa L. Southard ◽  
Andrew D. Miller
Keyword(s):  
Periodic Acid ◽  
Clinical Signs ◽  
Polarized Light ◽  
Granulomatous Inflammation ◽  
Crystal Violet Staining ◽  
Periodic Acid Schiff ◽  
Petaurus Breviceps ◽  
Oil Red O Staining ◽  
Oil Red O ◽  
Hyaline Material

Pulmonary hyalinosis is an idiopathic, typically incidental lesion of old dogs, characterized by multifocal aggregates of epithelioid and multinucleate macrophages that surround periodic acid–Schiff (PAS)-positive hyaline material in airways. Lung lesions resembling pulmonary hyalinosis were observed in 6 captive adult sugar gliders ( Petaurus breviceps; 5 females and 1 male) in a retrospective review of 18 autopsied animals. Clinical signs for 3 of the sugar gliders included lethargy, tachypnea, and dyspnea. At autopsy, 5 of 6 animals had comorbid lesions that were the primary cause of death. Gross pulmonary lesions were characterized by mildly firm, discolored, vaguely nodular areas of parenchyma. Histologic examination of the lung revealed granulomatous inflammation with intracellular and extracellular amphophilic hyaline bodies within alveoli and airways. Hyaline bodies were positive for PAS and oil red O staining, blue via crystal violet staining, and displayed birefringence under polarized light, similar to findings in dogs with pulmonary hyalinosis.

scholarly journals THE FINE STRUCTURE OF CEROID IN HUMAN ATHEROMA

1967 ◽  
Vol 15 (12) ◽  
pp. 732-736 ◽  
Author(s):  
FERENC GYÖRKEY ◽  
TETSUO SHIMAMURA ◽  
ROBERT M. O'NEAL
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Lamellar Structure ◽  
Periodic Acid ◽  
Periodic Acid Schiff ◽  
Histochemical Tests ◽  
Ultrathin Sections ◽  
Human Atheroma ◽  
Oil Red O ◽  
Electron Lucent

Ceroid in human atherosclerotic aorta was identified with histochemical tests (acid-fast, oil red O and periodic acid-Schiff techniques) applied to Epon-Araldite- and methacrylate-embedded tissues from which immediately adjacent ultrathin sections were studied with the electron microscope. The fine structure of ceroid in the human atherosclerotic aorta appears to be heterogeneous, showing uniquely wavy, irregularly arranged lamellae lying within granular material of varying electron density. The lamellar structure shows regular and alternating parallel electron-dense and electron-lucent zones which we believe to be characteristic of ceroid.

scholarly journals THE EFFECT OF SAPONIFICATION UPON CERTAIN HISTOCHEMICAL REACTIONS OF THE EPITHELIAL MUCINS OF THE GASTROINTESTINAL TRACT

1971 ◽  
Vol 19 (11) ◽  
pp. 654-662 ◽  
Author(s):  
C. F. A. CULLING ◽  
P. E. REID ◽  
W. L. DUNN
Keyword(s):  
Gastrointestinal Tract ◽  
Guinea Pig ◽  
Periodic Acid ◽  
Ileocecal Valve ◽  
Paraffin Sections ◽  
Periodic Acid Schiff ◽  
Fixed Tissue ◽  
Formalin Fixed Tissue ◽  
Cryostat Sections ◽  
Formalin Fixed

An increase in periodic acid-Schiff reactivity after saponification has been demonstrated in the gastrointestinal tract mucins of man, rat, rabbit and guinea pig. Tins pH-dependent phenomenon is independent of fixation and processing since it occurred in cryostat sections of fresh and formalin-fixed tissue and in paraffin sections of tissues fixed in eight different fixatives. In man and rat this effect occurs at the level of the ileocecal valve and below; in guinea pig it is confined to the large intestine and rectum; while in rabbits it occurs in Brunner's glands, the ileocecal valve and below. In man the increase is found throughout the crypts whereas in rabbits the effect is seen mainly at the luminal end; in rats it is seen largely at the base of the crypts; and in guinea pigs it is variable. This effect, due to an increase in the number of 1:2 glycol groups, is accompanied in man, rat and guinea pig by an increase in basophilia due to the presence of sulfate and/or other highly acidic groups.

Ultrastructure of the Parotid Gland of the Nine-Banded Armadillo

1977 ◽  
Vol 35 ◽  
pp. 640-641
Author(s):  
J. R. Ruby
Keyword(s):  
Endoplasmic Reticulum ◽  
Parotid Gland ◽  
Periodic Acid ◽  
Acinar Cells ◽  
Duct System ◽  
Parotid Glands ◽  
Dasypus Novemcinctus ◽  
Anterior Portion ◽  
Periodic Acid Schiff ◽  
Thin Sections

Parotid glands were obtained from five adult (four male and one female) armadillos (Dasypus novemcinctus) which were perfusion-fixed. The glands were located in a position similar to that of most mammals. They extended interiorly to the anterior portion of the submandibular gland.In the light microscope, it was noted that the acini were relatively small and stained strongly positive with the periodic acid-Schiff (PAS) and alcian blue techniques, confirming the earlier results of Shackleford (1). Based on these qualities and other structural criteria, these cells have been classified as seromucous (2). The duct system was well developed. There were numerous intercalated ducts and intralobular striated ducts. The striated duct cells contained large amounts of PAS-positive substance.Thin sections revealed that the acinar cells were pyramidal in shape and contained a basally placed, slightly flattened nucleus (Fig. 1). The rough endoplasmic reticulum was also at the base of the cell.

Ultrastructural Cytopiasmic Changes of Adrenal Cortex During Pregnancy

1969 ◽  
Vol 27 ◽  
pp. 298-299
Author(s):  
T. M. Murad ◽  
Karen Israel ◽  
Jack C. Geer
Keyword(s):  
Adrenal Gland ◽  
Steroid Hormones ◽  
Adrenal Cortex ◽  
Lipid Droplets ◽  
Plasma Cholesterol ◽  
Adrenal Steroids ◽  
Dynamic Changes ◽  
Cortical Cells ◽  
Acetyl Coenzyme A ◽  
Adrenal Cortical Cells

Adrenal steroids are normally synthesized from acetyl coenzyme A via cholesterol. Cholesterol is also shown to enter the adrenal gland and to be localized in the lipid droplets of the adrenal cortical cells. Both pregnenolone and progesterone act as intermediates in the conversion of cholesterol into steroid hormones. During pregnancy an increased level of plasma cholesterol is known to be associated with an increase of the adrenal corticoid and progesterone. The present study is designed to demonstrate whether the adrenal cortical cells show any dynamic changes during pregnancy.

Detection of Carriers in Glanzmann's Thrombasthenia

1983 ◽  
Vol 49 (03) ◽  
pp. 182-186
Author(s):  
G T E Zonneveld ◽  
E F van Leeuwen ◽  
A Sturk ◽  
J W ten Cate
Keyword(s):  
Bleeding Time ◽  
Periodic Acid ◽  
Type I ◽  
Clot Retraction ◽  
Periodic Acid Schiff ◽  
Glanzmann’S Thrombasthenia ◽  
Aggregation Response ◽  
Glanzmann's Thrombasthenia ◽  
Sds Page ◽  
Reduced State

SummaryQuantitative glycoprotein (GP) analysis of whole platelets or platelet membranes was performed by SDS-polyacrylamide gelelectrophoresis (SDS-PAGE) and periodic acid Schiff staining in the families of two unrelated Glanzmann’s thrombasthenia (GT) patients. Each family consisted of two symptom free parents, a symptom free daughter and a GT daughter. All symptom free members had a normal bleeding time, clot retraction and platelet aggregation response to adenosine 5’-diphosphate (ADP), collagen and adrenalin. Platelet Zw* antigen was normally expressed in these subjects. GT patiens, classified as a type I and II subject, showed reduced amounts of GP lib and of GP nia. Analysis of isolated membranes in the non-reduced state, however, showed that the amount of GP Ilia was also reduced in three of the four parents, whereas one parent (of the GT type I patient) and the two unaffected daughters had normal amounts of GP Ilia. Quantitative SDS-PAGE may therefore provide a method for the detection of asymptomatic carriers in GT type I and II.

Swim bladder mycosis in farmed rainbow trout Oncorhynchus mykiss caused by Phoma herbarum and experimental verification of pathogenicity

2020 ◽  
Vol 138 ◽  
pp. 237-246 ◽  
Author(s):  
J Řehulka ◽  
A Kubátová ◽  
V Hubka
Keyword(s):  
Rainbow Trout ◽  
Histopathological Examination ◽  
Periodic Acid ◽  
Bladder Wall ◽  
Swim Bladder ◽  
Cell Reactivity ◽  
Periodic Acid Schiff ◽  
Posterior Portion ◽  
Hepatic Congestion ◽  
Phoma Herbarum

In this study, spontaneous swim bladder mycosis was documented in a farmed fingerling rainbow trout from a raceway culture system. At necropsy, the gross lesions included a thickened swim bladder wall, and the posterior portion of the swim bladder was enlarged due to massive hyperplasia of muscle. A microscopic wet mount examination of the swim bladder contents revealed abundant septate hyphae, and histopathological examination showed periodic acid-Schiff-positive mycelia in the lumen and wall of the swim bladder. Histopathological examination of the thickened posterior swim bladder revealed muscle hyperplasia with expansion by inflammatory cells. The causative agent was identified as Phoma herbarum through morphological analysis and DNA sequencing. The disease was reproduced in rainbow trout fingerlings using intraperitoneal injection of a spore suspension. Necropsy in dead and moribund fish revealed extensive congestion and haemorrhages in the serosa of visceral organs and in liver and abdominal serosanguinous fluid. Histopathological examination showed severe hepatic congestion, sinusoidal dilatation, Kupffer cell reactivity, leukostasis and degenerative changes. Fungi were disseminated to the liver, pyloric caeca, kidney, spleen and heart. Although infections caused by Phoma spp. have been repeatedly reported in fish, species identification has been hampered by extensive taxonomic changes. The results of this study confirmed the pathogenicity of P. herbarum in salmonids by using a reliably identified strain during experimental fish infection and provides new knowledge regarding the course of infection.

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