scholarly journals Quantitative fluorescence spectrophotometry of acridine orange-stained unfixed cells. Potential for automated detection of human uterine cancer.

1976 ◽  
Vol 24 (1) ◽  
pp. 315-321 ◽  
Author(s):  
J F Golden ◽  
S S West ◽  
C K Echols ◽  
H M Shingleton
Keyword(s):  
Acridine Orange ◽  
Fluorescence Intensity ◽  
Uterine Cancer ◽  
Female Genital Tract ◽  
Fluorescence Emission ◽  
Emission Spectra ◽  
Cell Types ◽  
Fluorescence Emission Spectra ◽  
Acid Complex ◽  
Normal Cells

After staining with acridine orange (AO), the nuclei of unfixed cells from the human female genital tract exhibited the same fluorescence behavior previously observed for human and murine leukocytes and mouse ascites tumor cells. With staining conditions chosen to assure saturation of the green-fluorescing AO-nucleic acid complex in normal cells, corrected fluorescence emission spectra were recorded from the entire nucleus of 341 cells taken from 32 normal and 28 abnormal patients. Intensity of the recorded spectra was expressed in phosphor particle units, a fixed arbitrary unit of fluorescence intensity, to display intensity differences among the spectra from the various cell types. In all abnormal samples, one or more cells were found with 530-nm nuclear fluorescence intensity considerably greater than the maximum intensity recorded from normal cells. Determination of the adequacy of 530-nm nuclear fluorescence intensity as a criterion for cancer detection requires additional investigation. Additional criteria, if needed, may be supplied by the metachromasy of AO-stained unfixed cells.

scholarly journals Cytofluorometric and cytochemical comparisons of normal and abnormal human cells from the female genital tract.

1979 ◽  
Vol 27 (1) ◽  
pp. 591-595 ◽  
Author(s):  
J E Gill ◽  
L L Wheeless ◽  
C Hanna-Madden ◽  
R J Marisa
Keyword(s):  
Acridine Orange ◽  
Female Genital Tract ◽  
Fluorescence Emission ◽  
Cell Nuclei ◽  
Feulgen Staining ◽  
Fluorescence Emission Spectra ◽  
Acridine Orange Staining ◽  
Abnormal Cell ◽  
Dnase Treatment ◽  
Abnormal Cells

Acridine orange staining of exfoliated cells from epithelial tissues facilitates discrimination between normal and abnormal cells: abnormal cells develop highly elevated nuclear fluorescence. Comparisons of acridine orange (AO) staining with propidium iodide (PI) or Feulgen staining have shown that: (a) PI staining also provides highly elevated nuclear fluorescence from abnormal cells; (b) the distributions of nuclear fluorescence following AO or PI staining were usually not significantly different as judged by the Kolmogorov-Smirnov test; (c) fluorescence emission spectra from AO and PI stained cells are consistent with the hypothesis that both fluorochromes bind to DNA within cell nuclei; (d) DNAse treatment of AO stained normal cells eliminates the nuclear fluorescence peak from slit-scan contours; RNAse treatment has no effect on nuclear fluorescence; (e) the distribution of abnormal cell nuclear fluorescence after AO staining is usually, but not always, significantly different from the distribution of abnormal cell nuclear absorbance after Feulgen staining, with relative nuclear fluorescence being greater than relative nuclear absorbance. The hypothesis currently most consistent with these results is that elevated Feulgen DNA content can account for only part of the discrimination provided by AO staining, and that the chromatin within abnormal cells is altered so as to increase accessibility of DNA to intercalating dyes.

scholarly journals FLUORESCENCE SPECTROSCOPIC AND FADING BEHAVIOR OF EHRLICH'S HYPERDIPLOID MOUSE ASCITES TUMOR CELLS SUPRAVITALLY STAINED WITH ACRIDINE ORANGE

1974 ◽  
Vol 22 (7) ◽  
pp. 495-505 ◽  
Author(s):  
JAMES F. GOLDEN ◽  
SEYMOUR S. WEST
Keyword(s):  
Light Intensity ◽  
Acridine Orange ◽  
Tumor Cells ◽  
Fluorescence Emission ◽  
Emission Spectra ◽  
Exciting Light ◽  
Staining Reaction ◽  
Green Fluorescence ◽  
Fluorescence Emission Spectra ◽  
Mouse Ascites

Corrected fluorescence emission spectra and measurements of fluorescence intensity and fluorescence fading were recorded from Ehrlich's hyperdiploid mouse ascites (EHD) tumor cells supravitally stained with acridine orange (AO). After equilibration of the staining reaction, some EHD tumor cells with dye content greater than 5 x 10–15 mole AO/cell spectroscopically exhibit both red and green fluorescence while the rest fluoresce only green. This behavior contrasts with that previously observed for normal mouse leukocytes. Below 5 x 10–15 mole AO/cell, all EHD tumor cells fluoresce green, but the intensity varies considerably from cell to cell. The fading rate of the green fluorescence appears to be a linear function of fluorescence-exciting light intensity. Absolute light intensity measurements, a necessity for interexperiment comparisons and automation, were based upon a phosphor particle standard. The results presented are indicative of the possibilities for automated cytopathology that can be uncovered by rapid recording, corrected spectrum microspectrofluorophotometry and related biophysical cytochemical techniques.

Biochemical properties and ultrastructure of mesophyll and bundle sheath thylakoids from maize (Zea mays) chloroplasts

2020 ◽  
Vol 47 (11) ◽  
pp. 970
Author(s):  
Nahida K. Aliyeva ◽  
Durna R. Aliyeva ◽  
Saftar Y. Suleymanov ◽  
Fuad H. Rzayev ◽  
Eldar K. Gasimov ◽  
...  
Keyword(s):  
Zea Mays ◽  
Fluorescence Emission ◽  
Emission Spectra ◽  
Cell Types ◽  
Biochemical Properties ◽  
Photochemical Activity ◽  
Bundle Sheath ◽  
M Cells ◽  
Fluorescence Emission Spectra ◽  
Light Harvesting Complex

A characteristic feature of C4 plants is the differentiation of the photosynthetic leaf tissues into two distinct cell types: mesophyll (M) and bundle sheath (BS) cells. We have investigated several biochemical parameters, including pigment composition, polypeptide patterns, fluorescence at 77K, the activity of photosystems and ultrastructure of mesophyll and bundle sheath chloroplasts of maize (Zea mays L.) plants. It is shown that the BS chloroplasts have ~2-fold higher chlorophyll a/b ratio than M chloroplasts, 6.15 and 3.12 respectively. The PSI apoprotein (68 kDa) was more abundant in BS than in M thylakoids. Polypeptides belonging to PSII core antenna, are in similar amounts in both types of membranes, but the 45kDa band is more intensive in M thylakoids. Polypeptides in the region of 28–24 kDa of the light-harvesting complex of PSII (LHCII) are also present in both types of chloroplasts, though their amounts are reduced in BS thylakoids. The chlorophyll fluorescence emission spectra in M cells showed the presence of three bands at 686, 695 and 735 nm characteristics of LHCII, PSII core and PSI complexes, respectively. However, in the fluorescence spectrum of agranal plastids, there are almost traces of the band at 695 nm, which belongs to the PSII core complex. The research results revealed that the photochemical activity of PSII in BS chloroplasts is ~5 times less than in the chloroplasts of M cells. The highest PSI activity was found in maize BS chloroplasts.

scholarly journals One-Pot Hydrothermal Synthesis of Carbon Dots as Fluorescent Probes for the Determination of Mercuric and Hypochlorite Ions

Nanomaterials ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1831
Author(s):  
Hsin Lee ◽  
Yen-Chang Su ◽  
Hsiang-Hao Tang ◽  
Yu-Sheng Lee ◽  
Jan-Yee Lee ◽  
...  
Keyword(s):  
Fluorescence Intensity ◽  
Fluorescent Probes ◽  
Carbon Dots ◽  
Limit Of Detection ◽  
Tap Water ◽  
Fluorescence Emission ◽  
Emission Spectra ◽  
Excitation Wavelength ◽  
One Pot ◽  
Fluorescence Emission Spectra

Nitrogen and sulfur codoped carbon dots (NSCDs) were synthesized via a one-pot hydrothermal method, and citric acid, ethylenediamine, and methyl blue were used as precursors. The obtained NSCDs were spherical with an average size of 1.86 nm. The fluorescence emission spectra of the NSCDs were excitation independent and emitted blue fluorescence at 440 nm with an excitation wavelength at 350 nm. The quantum yield of the NSCDs was calculated to be 68.0%. The NSCDs could be constructed as fluorescent probes for highly selective and sensitive sensing mercuric (Hg2+) and hypochlorite (ClO−) ions. As the addition of Hg2+ or ClO− ions to the NSCDs, the fluorescence intensity was effectively quenched due to dynamic quenching. Under the optimal conditions, the linear response of the fluorescence intensity ranged from 0.7 μM to 15 μM with a detection limit of 0.54 μM and from 0.3 μM to 5.0 μM with a limit of detection of 0.29 μM for Hg2+ and ClO− ions, respectively. Finally, the proposed method was successfully used for quantifying Hg2+ and ClO− ions in spiked tap water samples.

scholarly journals Sponge-derived Ageladine A affects the in vivo fluorescence emission spectra of microalgae

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0242464
Author(s):  
Carolin Peter ◽  
Silke Thoms ◽  
Florian Koch ◽  
Franz Josef Sartoris ◽  
Ulf Bickmeyer
Keyword(s):  
Natural Products ◽  
Fluorescence Intensity ◽  
Crucial Role ◽  
Fluorescence Emission ◽  
Emission Spectra ◽  
Fluorescence Emission Spectra ◽  
Tisochrysis Lutea ◽  
Emission Maximum ◽  
Ph Dependent

In several marine hosts of microalgae, fluorescent natural products may play an important role. While the ecological function of these compounds is not well understood, an interaction of these molecules with the photosynthesis of the symbionts has been suggested. In this study, the effect of Ageladine A (Ag A), a pH-dependent fluorophore found in sponges of the genus Agelas, on microalgal fluorescence was examined. The spectra showed an accumulation of Ag A within the cells, but with variable impacts on fluorescence. While in two Synechococcus strains, fluorescence of phycoerythrin increased significantly, the fluorescence of other Synechococcus strains was not affected. In four out of the five eukaryote species examined, chlorophyll a (Chl a) fluorescence intensity was modulated. In Tisochrysis lutea, for example, the position of the fluorescence emission maximum of Chl a was shifted. The variety of these effects of Ag A on microalgal fluorescence suggests that fluorophores derived from animals could play a crucial role in shaping the composition of marine host/symbiont systems.

Optical Properties and Zinc Nitride Thin Films Prepared Using Magnetron Reactive Sputtering

2014 ◽  
Vol 940 ◽  
pp. 11-15
Author(s):  
Jun Qin Feng ◽  
Jun Fang Chen
Keyword(s):  
Thin Films ◽  
Band Gap ◽  
Near Infrared ◽  
Fluorescence Emission ◽  
Emission Spectra ◽  
Sem Images ◽  
Fluorescence Emission Spectra ◽  
Glass Substrates ◽  
Zinc Nitride ◽  
Direct Band

Zinc nitride films were deposited by ion sources-assisted magnetron sputtering with the use of Zn target (99.99% purity) on 7059 glass substrates. The films were characterized by XRD, SEM and EDS, the results of which show that the polycrystalline zinc nitride thin film can be grown on the glass substrates, the EDS spectrum confirmed the chemical composition of the films and the SEM images revealed that the zinc nitride thin films have a dense structure. Ultraviolet-visible-near infrared spectrophotometer was used to study the transmittance behaviors of zinc nitride thin films, which calculated the optical band gap by Davis Mott model. The results of the fluorescence emission spectra show the zinc nitride would be a direct band gap semiconductor material.

Stress and Phase Transformation Phenomena in Oxide Films: Real-Time Spectroscopic Measurements

1992 ◽  
Vol 271 ◽  
Author(s):  
Gregory J. Exarhos ◽  
Nancy J. Hess
Keyword(s):  
Phase Transformation ◽  
Applied Pressure ◽  
Oxide Films ◽  
Fluorescence Emission ◽  
Emission Spectra ◽  
Film Deposition ◽  
Optical Methods ◽  
Fluorescence Emission Spectra ◽  
Line Intensities ◽  
Deposition Parameters

ABSTRACTIn situ optical methods are reviewed for characterization of phase transformation processes and evaluation of residual stress in solution-deposited metastable oxide films. Such low density films most often are deposited as disordered phases making them prone to crystallization and attendant densification when subjected to increased temperature and/or applied pressure. Inherent stress imparted during film deposition and its evolution during the transformation are evaluated from phonon frequency shifts seen in Raman spectra (TiO2) or from changes in the laser-induced fluorescence emission spectra for films containing rare earth (Sm+3:Y3Al5O12) or transition metal (Cr+3 :Al2O3) dopants. The data in combination with measured increases in line intensities intrinsic to the evolving phase are used to follow crystallization processes in thin films. In general, film deposition parameters are found to influence the crystallite ingrowth kinetics and the magnitude of stress and stress relaxation in the film during the transformation. The utility of these methods to probe crystallization phenomena in oxide films will be addressed.

scholarly journals Fluorescence emission spectra of silver and silver/cobalt nanoparticles

2012 ◽  
Vol 19 (3) ◽  
pp. 943-947 ◽  
Author(s):  
Z. Parang ◽  
A. Keshavarz ◽  
S. Farahi ◽  
S.M. Elahi ◽  
M. Ghoranneviss ◽  
...  
Keyword(s):  
Fluorescence Emission ◽  
Emission Spectra ◽  
Cobalt Nanoparticles ◽  
Fluorescence Emission Spectra
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