scholarly journals LOCALIZATION WITHIN CLONED RAT PITUITARY TUMOR CELLS OF MATERIAL THAT BINDS ANTIGROWTH HORMONE ANTIBODY

1974 ◽  
Vol 22 (6) ◽  
pp. 385-394 ◽  
Author(s):  
SANDRA KAZAHN MASUR ◽  
ERIC HOLTZMAN ◽  
F. CARTER BANCROFT
Keyword(s):  
Growth Hormone ◽  
Endoplasmic Reticulum ◽  
Horseradish Peroxidase ◽  
Tumor Cells ◽  
Peroxidase Activity ◽  
Pituitary Cells ◽  
Binding Material ◽  
Rat Pituitary ◽  
Rat Pituitary Tumor Cells ◽  
Rat Pituitary Cells

Cultured rat pituitary cells known to synthesize growth hormone were fixed and exposed to antigrowth hormone antibody conjugated to horseradish peroxidase. The cells were then incubated to demonstrate peroxidase activity. Reaction product was present within the endoplasmic reticulum, including the nuclear envelope. Study of several types of control preparations strongly suggest that the antibody-binding material in the endoplasmic reticulum is growth hormone.

scholarly journals PRODUCTION OF BOTH PROLACTIN AND GROWTH HORMONE BY CLONAL STRAINS OF RAT PITUITARY TUMOR CELLS

1970 ◽  
Vol 47 (1) ◽  
pp. 61-70 ◽  
Author(s):  
Armen H. Tashjian ◽  
Frank C. Bancroft ◽  
Lawrence Levine
Keyword(s):  
Growth Hormone ◽  
Mammalian Cells ◽  
Cell Protein ◽  
Pituitary Cells ◽  
Hormone Production ◽  
Rat Pituitary ◽  
Organ Specific ◽  
Cell Strains ◽  
Rat Pituitary Tumor Cells ◽  
Rat Pituitary Cells

Several established clonal strains of rat pituitary cells which produce growth hormone in culture have been shown to secrete a second protein hormone, prolactin. Prolactin was measured immunologically in culture medium and within cells by complement fixation. Rates of prolactin production varied from 6.6 to 12 µg/mg cell protein per 24 hr in four different cell strains. In these cultures ratios of production of prolactin to growth hormone varied from 1.0 to 4.1. A fifth clonal strain produced growth hormone but no detectable prolactin. Intracellular prolactin was equivalent to the amount secreted into medium in a period of about 1–2 hr. Both cycloheximide and puromycin suppressed prolactin production by at least 94%. Hydrocortisone (3 x 10-6 M), which stimulated the production of growth hormone 4- to 8-fold in most of the cell strains, reduced the rate of prolactin production to less than 25% of that in control cultures. Conversely, addition of simple acid extracts of several tissues, including hypothalamus, to the medium of all strains increased the rate of production of prolactin six to nine times and decreased growth hormone production by about 50%. We conclude that multifunctional rat pituitary cells in culture show unusual promise for further studies of the control of expression of organ-specific activities in mammalian cells.

PACAP 38 regulates prolactin (PRL) and growth hormone (GH) secretion and gene expression in cultured rat pituitary cells

1992 ◽  
Vol 40 (2) ◽  
pp. 289
Author(s):  
L. Zheng ◽  
M. Kazemzadeh ◽  
B. Velkeniers ◽  
A. Vandermeers ◽  
J. Christophe ◽  
...  
Keyword(s):  
Gene Expression ◽  
Growth Hormone ◽  
Pituitary Cells ◽  
Gh Secretion ◽  
Rat Pituitary ◽  
Rat Pituitary Cells
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