scholarly journals SENSITIVITY IN ELECTRON MICROSCOPE AUTORADIOGRAPHY FOR 125I

1974 ◽  
Vol 22 (2) ◽  
pp. 80-87 ◽  
Author(s):  
HELEN C. FERTUCK ◽  
MIRIAM M. SALPETER
Keyword(s):  
Heavy Metal ◽  
Electron Microscope ◽  
Radiation Dose ◽  
Dose Dependence ◽  
Specimen Thickness ◽  
Section Thickness ◽  
Electron Microscope Autoradiography ◽  
Absorption Studies ◽  
Microscope Autoradiography ◽  
Better Than

Sensitivity in electron microscope autoradiography was determined for 125I. Values are given using Ilford L4 and Kodak NTE emulsions combined with different developers. The extent of self-absorption as a function of section thickness and heavy metal staining and the effect of radiation dose (dose dependence) were assessed. It was found that the over-all efficiency for 125I was better than that for tritium and that, as is the case with tritium, there is a distinct "dose dependence" especially when Microdol X is the developer. Self-absorption studies indicate that self-absorption is increased by about 15% when the specimen thickness is increased from 300 to 1000 Å. An increase of under 15% is also introduced by heavy metal staining of sections in this thickness range.

scholarly journals SENSITIVITY IN ELECTRON MICROSCOPE AUTORADIOGRAPHY I. THE EFFECT OF RADIATION DOSE

1972 ◽  
Vol 20 (6) ◽  
pp. 425-434 ◽  
Author(s):  
MIRIAM M. SALPETER ◽  
MARIA SZABO
Keyword(s):  
Ascorbic Acid ◽  
Electron Microscope ◽  
Radiation Dose ◽  
Surface Area ◽  
Test Specimen ◽  
Dose Dependence ◽  
Unit Surface Area ◽  
Electron Microscope Autoradiography ◽  
Microscope Autoradiography ◽  
Unit Surface

Sensitivity in electron microscope autoradiography using Ilford L4 emulsion was shown to be affected by radiation dose ( i.e., number of decays in test specimen per unit surface area). The sensitivity tended to be higher with lower doses. This dose dependence was most marked with Microdol X and least with gold latensification-Elon ascorbic acid development. Possible consequences for quantitation in electron microscope autoradiography are discussed.

scholarly journals SENSITIVITY IN ELECTRON MICROSCOPE AUTORADIOGRAPHY II. EFFECT OF HEAVY METAL STAINING

1973 ◽  
Vol 21 (7) ◽  
pp. 623-627 ◽  
Author(s):  
MIRIAM M. SALPETER
Keyword(s):  
Heavy Metals ◽  
Heavy Metal ◽  
Electron Microscope ◽  
Uranyl Acetate ◽  
Protein Films ◽  
Section Thickness ◽  
Electron Microscopic ◽  
Electron Microscope Autoradiography ◽  
Electron Microscopic Autoradiography ◽  
Microscope Autoradiography

In quantitative electron microscopic autoradiography test specimens for sensitivity usually consist of plastic or protein films 500-1000 Å in thickness. For tritium, the applicability of sensitivity values derived from such specimens to biologic sections which had been stained with heavy metals was determined. It was found that, within the range of section thickness used for electron microscopic autoradiography, fixation with OsO4 followed by uranyl acetate staining increases self-absorption by less than 7% over that seen in Epon sections of the same thickness. A somewhat larger effect is seen in sections used for light microscopic autoradiography. The effect on electron microscopic autoradiographic resolution is estimated to be within 10%.

scholarly journals Sensitivity in electron microscope autoradiography for calcium-45.

1980 ◽  
Vol 28 (1) ◽  
pp. 40-44 ◽  
Author(s):  
W V Harris ◽  
M M Salpeter
Keyword(s):  
Electron Microscope ◽  
Radiation Dose ◽  
Double Layers ◽  
Electron Microscope Autoradiography ◽  
Microscope Autoradiography

The sensitivity of Ilford L4 emulsion to 45Ca was determined for electron microscope autoradiographic conditions. Sensitivity values were obtained for monolayers and double layers of emulsion in combination with various developing procedures. The dependence of sensitivity upon radiation dose was determined. All results are compared to previously calibrated isotopes.

scholarly journals ERRATUM

1972 ◽  
Vol 20 (11) ◽  
pp. 967-967
Keyword(s):  
Electron Microscope ◽  
Radiation Dose ◽  
Electron Microscope Autoradiography ◽  
Microscope Autoradiography

In the paper by Salpeter and Szabo entitled "Sensitivity in Electron Microscope Autoradiography. I. The Effect of Radiation Dose," which appeared in the June issue of the Journal (Vol. 20, pp. 425-434, 1972), the lines 28 and 29 of column 2 on page 427 should read, "2. In a second method, sections of tritiated methacrylate (10 mC/g or 400 µC/g) were used...."

Incorporation of Cholesterol into Peripheral Nerve Myelin

1972 ◽  
Vol 30 ◽  
pp. 334-335
Author(s):  
Frank A. Rawlins
Keyword(s):  
Electron Microscope ◽  
Sciatic Nerve ◽  
Electron Microscope Autoradiography ◽  
Myelin Sheaths ◽  
Microscope Autoradiography ◽  
Grain Distribution ◽  
Sciatic Nerves ◽  
Autoradiographic Analysis ◽  
Old Mice ◽  
Short Times

Several speculations exist as to the site of incorporation of preformed molecules into myelin. The possibility that an autoradiographic analysis of cholesterol-1,2-H3 incorporation at very short times after injection might shed some light in the solution of that problem led to the present experiment.Cholesterol-1,2-H3 was injected intraperitoneally into 24 tenday old mice. The animals were then sacrificed at 10,20,30,40,60,90,120 and 180 min after the injection and the sciatic nerves were processed for electron microscope autoradiography. To analyze the grain distribution in the autoradiograms of cross and longitudinal sections from each sciatic nerve myelin sheaths were subdivided into three compartments named: outer 1/3, middle 1/3 and inner 1/3 compartments.It was found that twenty min. after the injection of cholesterol -1.2-H3 (Figs. 1 and 2), 55% of the total number of grains (t.n.g) found in myelin were within the outer 1/3 compartment, 9% were within the middle 1/3 and 36% within the inner 1/3 compartment

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