scholarly journals IMMUNOFLUORESCENT LOCALIZATION OF α2u-GLOBULIN IN THE HEPATIC AND RENAL TISSUES OF RAT

1972 ◽  
Vol 20 (2) ◽  
pp. 89-96 ◽  
Author(s):  
ARUN K. ROY ◽  
DOUGLAS L. RABER
Keyword(s):  
Male Rat ◽  
Loop Of Henle ◽  
Immunofluorescent Localization ◽  
Collecting Ducts ◽  
Particulate Form ◽  
Tubular Lumen ◽  
Adult Male Rat ◽  
Renal Tubular ◽  
Parenchymal Cells ◽  
Convoluted Tubules

α2u-Globulin is an androgen-inducible rat urinary protein of hepatic origin. Examination of adult male rat liver sections with rhodamine-labeled anti-α2u-antiserum showed that the liver parenchymal cells are the site of α2u-globulin synthesis and that all of the parenchymal cells contain this protein. Immunofluorescent localization of α2u-globulin in male kidney sections revealed the existence of the protein in the cells of proximal convoluted tubules, in the loop of Henle as well as in the distal convoluted tubule. The collecting ducts did not bind the fluorescent labeled antibody. Intraperitoneally administered rhodamine-labeled α2u-globulin was found to be localized only in the kidney tubules in particulate form. It is concluded that α2u-globulin after its synthesis and release from the hepatic parenchymal cells is filtered into the renal tubular lumen and that both kinds of convoluted tubules as well as the loop of Henle are actively involved in α2u reabsorption.

scholarly journals Study the Anatomical Descriptions and Histological Observations of the Kidney in Golden Eagles (Aquila Chrysaetos)

2012 ◽  
Vol 36 (2) ◽  
pp. 145-152
Author(s):  
Ramzi Abdul ghafoor Abood AL-Agele
Keyword(s):  
Mesangial Cells ◽  
Apical Cell ◽  
Kidney Tissue ◽  
Middle Lobe ◽  
Golden Eagles ◽  
Collecting Ducts ◽  
Tubular Lumen ◽  
Membrane Bound ◽  
Collecting Tubules ◽  
Convoluted Tubules

The present study is preliminary investigate of the kidney gross morphology and some histological observations was studied in golden eagle (Aquila Chrysaetus). The anatomical descriptions of the kidneys had revealed a paired kidney and ureters were protrude, flattened and extra-peritoneal organs. The kidney tissue is transverse by major nerve trunks and blood vessels, securing the kidneys tightly in place. Each kidney consists of three lobes: cranial, middle and caudal lobes. The cranial lobe was the largest and wider than the other two lobes and the caudal lobe was appeared similar to middle lobe but little smaller than it. There is no line of demarcation between cortex and medulla as in mammals. The glomerulus consisted of a tightly packed central core of mesangial cells, surrounded by capillary loops. The cytoplasm of the proximal convoluted tubules cells contains condensing vesicles and a large apically situated nucleus. The distal convoluted tubules and collecting ducts were distinguished on a topographical basis revealed that varied slightly in their reactions with PAS stain and appeared as vacuoles or look like vesicles contains secretion from lining epithelial cells of distal convoluted and collecting tubules which appeared diffused in cross section. The cells of distal convoluted tubules and collecting tubules possessed vesicles with a clearly defined coated outer membrane and some had small blebs invaginated membrane. However in this study found vesicles or vacuoles in the lumen of collecting tubules revealed the secreting cells had margins that were often incompletely membrane –bound and continuous with the apical cell margin as if discharging their contents to the tubular lumen.

RT-PCR study of the distribution of connexin 43 mRNA in the glomerulus and renal tubular segments

1998 ◽  
Vol 275 (2) ◽  
pp. R439-R447 ◽  
Author(s):  
Rong Guo ◽  
Li Liu ◽  
Luciano Barajas
Keyword(s):  
Connexin 43 ◽  
Mrna Level ◽  
Thick Ascending Limb ◽  
Rt Pcr ◽  
High Concentration ◽  
Medullary Thick Ascending Limb ◽  
Collecting Ducts ◽  
Cx43 Mrna ◽  
Renal Tubular ◽  
Convoluted Tubules

An RT-PCR study of the distribution of connexin 43 (Cx43) mRNA in glomeruli and along the rat tubular segments was carried out to establish the differential expression of Cx43 in the different segments of the tubule, in renal regions, in isolated glomerular preparations (IGP), and in microdissected glomeruli. The mRNA level of Cx43 in macrodissected renal regions appeared in the following order: inner papilla > outer papilla and IGP > outer medulla and cortex. Among the microdissected tubules, inner medullary collecting ducts (IMCD) expressed the highest level of Cx43 mRNA, followed by the cortical collecting ducts (CCD). The proximal convoluted tubules and proximal straight tubules expressed significantly less Cx43 than the IMCD, glomeruli, and CCD. Medullary thick ascending limb and distal convoluted tubules showed the lowest level of Cx43 mRNA. The RT-PCR results of the microdissected segments correlate well with those obtained by RT-PCR of the renal regions. The high concentration of Cx43 mRNA in the IMCD together with the observation of abundant punctate immunofluorescence for Cx43 suggests that the IMCD not only expresses Cx43 mRNA but also that the mRNA is translated to Cx43 protein.

Physical Properties of Isolated Perfused Renal Tubular Basement Membranes

1971 ◽  
Vol 29 ◽  
pp. 390-391
Author(s):  
Jared Grantham ◽  
Larry Welling
Keyword(s):  
Basement Membrane ◽  
Basement Membranes ◽  
Transport Mechanisms ◽  
Permeability Characteristics ◽  
Peritubular Capillaries ◽  
Strategic Location ◽  
Tubular Lumen ◽  
Glomerular Filtrate ◽  
Urine Formation ◽  
Renal Tubular

In the course of urine formation in mammalian kidneys over 90% of the glomerular filtrate moves from the tubular lumen into the peritubular capillaries by both active and passive transport mechanisms. In all of the morphologically distinct segments of the renal tubule, e.g. proximal tubule, loop of Henle and distal nephron, the tubular absorbate passes through a basement membrane which rests against the basilar surface of the epithelial cells. The basement membrane is in a strategic location to affect the geometry of the tubules and to influence the movement of tubular absorbate into the renal interstitium. In the present studies we have determined directly some of the mechanical and permeability characteristics of tubular basement membranes.

Overtraining of aerobic physical exercise reduce rats (Rattus norvegicus) memory

2020 ◽  
Vol 14 (1) ◽  
Author(s):  
Ni Made Ridla Parwata
Keyword(s):  
Body Weight ◽  
Physical Exercise ◽  
Rattus Norvegicus ◽  
Research Method ◽  
Male Rat ◽  
Control Group ◽  
Adult Male Rat ◽  
The Subject ◽  
Heavy Training

Overtraining syndrome is a decrease in physical capacity, emotions and immunity due to training that is too often without adequate periods of rest. Overtraining is often experienced by athletes who daily undergo heavy training with short break periods. This research aims to look at the effect of overtraining aerobic physical exercise on memory in mice. The research method was experimental in vivo with the subject of adult male rat (Rattus Norvegicus) Winstar strain aged 8-10 weeks, body weight 200-250 gr. Divided into three groups, namely the control group, aerobic group and overtraining group. The results of memory tests with water E Maze showed an increase in the duration of travel time and the number of animal errors made by the overtraining group (p = 0.003). This study concludes that overtraining aerobic physical exercise can reduce memory in rat hippocampus.

scholarly journals Tenofovir and Severe Symptomatic Hypophosphatemia

2019 ◽  
Vol 7 ◽  
pp. 232470961984879 ◽  
Author(s):  
Asim Kichloo ◽  
Savneek Singh Chugh ◽  
Sanjeev Gupta ◽  
Jay Panday ◽  
Ghazaleh Goldar
Keyword(s):  
Renal Damage ◽  
Organic Anion ◽  
Significant Risk ◽  
Anion Transporters ◽  
Immunodeficiency Virus ◽  
Tubular Lumen ◽  
Associated Proteins ◽  
Proximal Tubular ◽  
Renal Tubular ◽  
Initial Results

Tenofovir is a broadly used drug used for the treatment of human immunodeficiency virus (HIV). Although the initial results of the clinical trials supported the renal safety of Tenofovir, clinical use of it has caused a low, albeit a significant, risk of renal damage either in the form of AKI or CKD. The pathophysiology has been linked to the effect of this medication on the proximal tubular cell. Although the exact mechanism is unknown, studies have suggested that Tenofovir accumulates in proximal tubular cells which are rich in mitochondria. It is both filtered in the glomerulus and actively secreted in the tubules for elimination and is excreted unchanged in the urine. Studies have shown an active transportation of 20-30% of this drug into the renal proximal tubule (PCT) cells via the organic anion transporters in the baso-lateral membrane (primarily hOAT1, and OAT3 to a lesser extent) and ultimate excretion of the drug into the tubular lumen via the transporters in the proximal tubular apical membrane MRP4 and MRP2 (multidrug resistance-associated proteins 2 & 4). Subsequently, the mitochondrial injury caused by Tenofovir can lead to the development of Fanconi’s syndrome which causes renal tubular acidosis, phosphaturia, aminoaciduria, glucosuria with normoglycemia, and tubular proteinuria. Here we present a case where Tenofovir treatment resulted in severe hypophosphatemia requiring hospitalization for parentral phosphate repletion.

scholarly journals Maternal tobacco smoke increased visceral adiposity and serum corticosterone levels in adult male rat offspring

2014 ◽  
Vol 76 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Erin K. Zinkhan ◽  
Brook Y. Lang ◽  
Baifeng Yu ◽  
Yan Wang ◽  
Chengshe Jiang ◽  
...  
Keyword(s):  
Adult Male ◽  
Tobacco Smoke ◽  
Visceral Adiposity ◽  
Male Rat ◽  
Serum Corticosterone ◽  
Rat Offspring ◽  
Adult Male Rat

scholarly journals An immunohistochemical study of aspartate, glutamate, and taurine in rat kidney.

1994 ◽  
Vol 42 (5) ◽  
pp. 621-626 ◽  
Author(s):  
N Ma ◽  
E Aoki ◽  
R Semba
Keyword(s):  
Amino Acids ◽  
Renal Cortex ◽  
Rat Kidney ◽  
Renal Medulla ◽  
Loop Of Henle ◽  
Immunoperoxidase Method ◽  
Collecting Tubules ◽  
Convoluted Tubules ◽  
Thin Portion ◽  
Intrarenal Distribution

Biochemical studies have revealed considerable amounts of free amino acids in the kidney. We examined the intrarenal distribution of three amino acids (aspartate, glutamate, and taurine) in the rat kidney with an immunoperoxidase method. In the renal cortex, all three amino acids were concentrated in the renal corpuscles and in the epithelia of the collecting tubules. Immunostaining of the collecting tubules was more intense in the principal cells than in the intercalated cells. The distal convoluted tubules were also immunostained with aspartate- and glutamate- specific antibodies but not with the taurine-specific antibody. In the renal medulla, the immunoreactivity specific for aspartate and for glutamate was similar; it was weak in the thick portion of the loop of Henle and strong in the collecting tubules. Immunoreactivity specific for taurine was restricted to regions within the epithelia of the thin portion of the loop of Henle and the collecting tubules. The significance of the accumulated amino acids as osmoregulatory agents is discussed.

15-Day intact adult male rat assay with alpha-cypermethrin

2016 ◽  
Vol 258 ◽  
pp. S308-S309 ◽  
Author(s):  
M. Kemény ◽  
H.A. Marxfeld ◽  
R. Buesen ◽  
A. Chukwudebe ◽  
B. Van Ravenzwaay ◽  
...  
Keyword(s):  
Adult Male ◽  
Male Rat ◽  
Adult Male Rat
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