scholarly journals DEMONSTRATION OF HISTONES IN PROERYTHROBLASTS IN PERNICIOUS ANEMIA AND THE DI GUGLIELMO SYNDROME

1972 ◽  
Vol 20 (10) ◽  
pp. 817-820 ◽  
Author(s):  
LAWRENCE KASS
Keyword(s):  
Pernicious Anemia ◽  
Silver Staining ◽  
Fast Green ◽  
Speckled Pattern ◽  
Ammoniacal Silver

Cytochemical studies on histones of proerythroblasts from patients with pernicious anemia and the Di Guglielmo syndrome were performed. Reduced amounts of histones were found in the proerythroblasts of pernicious anemia using the alkaline fast green stain. Ammoniacal silver staining indicated predominantly lysine-rich histone in the nuclei of pernicious anemia proerythroblasts. Using the same stain, the nuclei of Di Guglielmo proerythroblasts demonstrated a "speckled" pattern, indicating discrete deposits of arginine-rich histone. The relationships which may exist between these findings and the pathogenesis of megaloblastic and megaloblastoid erythropoiesis are discussed.

scholarly journals HISTONE SPECIFICITY REVEALED BY AMMONIACAL SILVER STAINING

1966 ◽  
Vol 14 (2) ◽  
pp. 177-181 ◽  
Author(s):  
MAURICE M. BLACK ◽  
HUIDSON R. ANSLEY
Keyword(s):  
Silver Staining ◽  
Staining Method ◽  
Cell Types ◽  
Color Differences ◽  
Different Cell Types ◽  
Ammoniacal Silver

Post-formalin ammoniacal-silver, a staining method which is selective for histone, imparts different colors, ranging from yellowish to black, to many different cell types. The same colors are imparted to histone extracts of the cell types in question. Pure lysine as well as so-called lysine-rich histone subfractions stain yellowish, while pure arginine and so-called arginine-rich subfractions stain black. The mechanism of the color differences of the stain is somewhat elucidated by these findings, and the study of histone specificity facilitated.

scholarly journals AMMONIACAL SILVER STAINING OF THE ERYTHROPOIETIC CELLS OF THE CHICK

1968 ◽  
Vol 16 (2) ◽  
pp. 148-150 ◽  
Author(s):  
GERALD D. MEETZ ◽  
EDITH K. MACRAE
Keyword(s):  
Silver Staining ◽  
Ammoniacal Silver

scholarly journals Improved mass spectrometry compatibility is afforded by ammoniacal silver staining

PROTEOMICS ◽  
2006 ◽  
Vol 6 (8) ◽  
pp. 2350-2354 ◽  
Author(s):  
Mireille Chevallet ◽  
Hélène Diemer ◽  
Sylvie Luche ◽  
Alain van Dorsselaer ◽  
Thierry Rabilloud ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Silver Staining ◽  
Ammoniacal Silver

Ammoniacal silver staining of proteins: Mechanism of glutaraldehyde enhancement

1983 ◽  
Vol 129 (2) ◽  
pp. 490-496 ◽  
Author(s):  
Arnold S. Dion ◽  
Anthony A. Pomenti
Keyword(s):  
Silver Staining ◽  
Ammoniacal Silver

scholarly journals Inflorescence Initiation In Lolium Temulentum l. VIII. Histochemical Changes At the Shoot Apex During Induction

1966 ◽  
Vol 19 (2) ◽  
pp. 233 ◽  
Author(s):  
RB Knox ◽  
IT Evans
Keyword(s):  
Silver Nitrate ◽  
Shoot Apex ◽  
Nuclear Proteins ◽  
Bromophenol Blue ◽  
Fast Green ◽  
Dna And Rna ◽  
Long Day ◽  
Orange Fluorescence ◽  
Histochemical Changes ◽  
Ammoniacal Silver

Shoot apices of Lotium temulentum plants exposed to 1 long day were harvested at the beginning of the long day, and on each of the five following days. Longitudinal sections were stained for DNA (Feulgen), DNA and RNA (methyl greenpyronin or acridine orange fluorescence), or basic nuclear proteins (ammoniacal silver nitrate, fast green, or bromophenol blue, with or without acetylation or deamination).

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