scholarly journals THE VITAMIN A CONTENT AND RETINOL ESTERIFYING ACTIVITY OF A KUPFFER CELL FRACTION OF RAT LIVER

1972 ◽  
Vol 20 (10) ◽  
pp. 811-816 ◽  
Author(s):  
SAMUEL H. HORI ◽  
TAKAKO KITAMURA
Keyword(s):  
Vitamin A ◽  
Rat Liver ◽  
Kupffer Cell ◽  
Kupffer Cells ◽  
Qualitative Data ◽  
Fluorescence Microscope ◽  
Cell Fraction ◽  
Frozen Sections ◽  
Main Site ◽  
Formalin Fixed

Kupffer cells were isolated from normal and A-hypervitaminotic rats, and their vitamin A content and retinol esterifying activity were assayed in order to examine whether the Kupffer cells actually participate in the storage of vitamin A. The intrahepatic distribution of vitamin A was also studied using frozen sections of fresh and formalin-fixed livers by means of the fluorescence microscope. Both quantitative and qualitative data indicate that Kupffer cells are not the main site of vitamin A storage. The fluorescence of vitamin A and its reactivity to SbCl3 decreased drastically after formalin treatment.

scholarly journals A new method to monitor Kupffer-cell function continuously in the perfused rat liver. Dissociation of glycogenolysis from particle phagocytosis

1990 ◽  
Vol 266 (1) ◽  
pp. 141-147 ◽  
Author(s):  
K B Cowper ◽  
R T Currin ◽  
T L Dawson ◽  
K A Lindert ◽  
J J Lemasters ◽  
...  
Keyword(s):  
Rat Liver ◽  
Kupffer Cell ◽  
Kupffer Cells ◽  
Cell Function ◽  
New Method ◽  
Carbon Uptake ◽  
Prostaglandin D2 ◽  
Perfused Rat Liver ◽  
Colloidal Carbon ◽  
Parenchymal Cells

In order to study particle phagocytosis and glycogenolysis simultaneously, this study was designed to develop a direct-read-out method to monitor Kupffer-cell function continuously, based on the uptake of colloidal carbon by the isolated perfused rat liver. Livers were perfused for 20 min with Krebs-Henseleit buffer saturated with O2/CO2 (19:1). Colloidal carbon (1-2 mg/ml) was added to the buffer, and absorbance of carbon was monitored continuously at 623 nm in the effluent perfusate. Since colloidal-carbon uptake was proportional to A623, rates of uptake were determined from the influent minus effluent concentration difference, the flow rate and the liver wet weight. Rates of colloidal-carbon uptake were 50-200 mg/h per g and were proportional to the concentration of carbon infused. Data from light-microscopy and cell-separation studies demonstrated that carbon was taken up exclusively by non-parenchymal cells and predominantly by Kupffer cells. Further, the amount of colloidal carbon detected histologically in non-parenchymal cells increased as the concentration of colloidal carbon in the perfusate was elevated. When Kupffer cells were activated or inhibited by treatment with endotoxin or methyl palmitate, carbon uptake was increased or decreased respectively. Taken together, these results indicate that Kupffer-cell function can be monitored continuously in a living organ. This new method was utilized to compare the time course of phagocytosis of carbon by Kupffer cells and carbohydrate output by parenchymal cells. Carbohydrate output increased rapidly by 69 +/- 9 mumol per g within 2-4 min after addition of carbon and returned to basal values within 12-16 min. However, carbon uptake by the liver did not reach maximal rates until about 15 min. Infusion of a cyclo-oxygenase inhibitor, aspirin (10 mM), caused a progressive decrease in carbohydrate output and blocked the stimulation by carbon completely. Aspirin neither altered rates of carbon uptake nor prevented stimulation of carbohydrate release by addition of N2-saturated buffer. The data from these experiments are consistent with the hypothesis that output of mediators by Kupffer cells, presumably prostaglandin D2 and E2, occurs transiently as Kupffer cells begin to phagocytose foreign particles in the intact organ, a process which continues at high rates for hours.

scholarly journals Quantitative Distribution of Vitamin A in Kupffer Cell and Hepatocyte Populations of Rat Liver

1971 ◽  
Vol 246 (17) ◽  
pp. 5538-5540 ◽  
Author(s):  
Maria C. Linder ◽  
G. Harvey Anderson ◽  
Immanuel Ascarelli
Keyword(s):  
Vitamin A ◽  
Rat Liver ◽  
Kupffer Cell ◽  
Quantitative Distribution

Ultrastructural Localization of Acid Mucosubstance in Skeletal Muscle

1967 ◽  
Vol 25 ◽  
pp. 52-53 ◽  
Author(s):  
William J. Dougherty ◽  
Samuel S. Spicer
Keyword(s):  
Striated Muscle ◽  
Divalent Cations ◽  
Ultrastructural Localization ◽  
Major Elements ◽  
Frozen Sections ◽  
Thorium Dioxide ◽  
Iron Solution ◽  
Coupling System ◽  
Psoas Muscles ◽  
Formalin Fixed

In recent years, considerable attention has focused on the morphological nature of the excitation-contraction coupling system of striated muscle. Since the study of Porter and Palade, it has become evident that the sarcoplastic reticulum (SR) and transverse tubules constitute the major elements of this system. The problem still exists, however, of determining the mechamisms by which the signal to interdigitate is presented to the thick and thin myofilaments. This problem appears to center on the movement of Ca++ions between myofilaments and SR. Recently, Philpott and Goldstein reported acid mucosubstance associated with the SR of fish branchial muscle using the colloidal thorium dioxide technique, and suggested that this material may serve to bind or release divalent cations such as Ca++. In the present study, Hale's iron solution adapted to electron microscopy was applied to formalin-fixed myofibrils isolated from glycerol-extracted rabbit psoas muscles and to frozen sections of formalin-fixed rat psoas muscles.

scholarly journals Crystallization of and preliminary X-ray data for an intracellular vitamin A-binding protein from rat liver.

1981 ◽  
Vol 256 (15) ◽  
pp. 8162-8163 ◽  
Author(s):  
M.E. Newcomer ◽  
A. Liljas ◽  
U. Eriksson ◽  
J. Sundelin ◽  
L. Rask ◽  
...  
Keyword(s):  
Vitamin A ◽  
Rat Liver ◽  
Binding Protein ◽  
X Ray

scholarly journals Immunohistochemical Detection of Brucella Melitensis Antigens in Cases of Naturally Occurring Abortions in Sheep

2008 ◽  
Vol 20 (6) ◽  
pp. 803-806 ◽  
Author(s):  
Fatma Ilhan ◽  
Zabit Yener
Keyword(s):  
Kupffer Cells ◽  
Brucella Melitensis ◽  
Immunohistochemical Detection ◽  
Zoonotic Pathogen ◽  
Sheep And Goats ◽  
Naturally Occurring ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Microscopic Studies ◽  
Formalin Fixed

Brucella melitensis, a worldwide zoonotic pathogen, is a significant cause of abortion in sheep and goats in some countries. The present study was carried out to determine, by immunohistochemistry, the presence of B. melitensis antigens in 110 naturally occurring aborted sheep fetuses. Sections of lung, liver, kidney, and spleen of each fetus were stained with immunoperoxidase to detect Brucella antigens. Brucella melitensis antigens were detected in 33 of 110 fetuses (30%). In the 33 positive cases, Brucella antigens were found in lung (25 [22.7%]), liver (21 [19%]), spleen (13 [11.8%]), and kidney (6 [5.4%]). Microscopic studies demonstrated that Brucella antigens were mainly located in the cytoplasm of macrophages and neutrophils of the lung, and in the cytoplasm of macrophages in the portal infiltrates and Kupffer cells of the liver. It was concluded that immunohistochemistry in formalin-fixed, paraffin-embedded tissues is a useful tool for the diagnosis of spontaneous ovine abortion caused by B. melitensis.

Vitamin A Ester and Alcohol of Rat Liver During Pregnancy.

1963 ◽  
Vol 112 (3) ◽  
pp. 605-608
Author(s):  
W. N. Dannenburg ◽  
D. S. Dixon ◽  
R. L. Burt
Keyword(s):  
Vitamin A ◽  
Rat Liver
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