scholarly journals Sperm Parameters, ASAs and Apoptosis After Processing by the Double Tube and Swim up Methods

2021 ◽  
Vol 15 (2) ◽  
pp. 155798832110012
Author(s):  
Zhi-Da Shi ◽  
Yan-Ping Zhang ◽  
Li-Ping Zhai ◽  
Mei-Hua Zhang ◽  
Yun-Ling Dong ◽  
...  
Keyword(s):  
Western Blotting ◽  
Caspase 3 ◽  
Abortion Rate ◽  
Dna Integrity ◽  
Antisperm Antibodies ◽  
Cleavage Rate ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Ivf Et

The aim of this study was to improve the quality of semen samples by using a novel double-tube (DT) method. The DT method was developed to select sperm and compared with traditional swim-up (SU) technique for 31 semen samples. Sperm DNA integrity were tested with TUNEL and SCSA. Content of antisperm antibodies (ASA) in the semen was measured by ELISA and MAR. Levels of the caspase-3 in the sperm were assessed by western blotting. After SU and DT, 15 couples and 16 couples were underwent IVF-ET. The number of RCDs, the percentage of SDF and DFI, ASA and the level of caspase-3 were significantly decreased after DT and SU ( p = .001 and p < .001). When the DT and SU compared, there were significant changes in the number of RCD, the percentage of SDF and DFI, ASA and the level of caspase-3 ( p < 0.05-0.001). There was a higher cleavage rate ( p = .017) and a lower abortion rate ( p < .05) in DT-IVF group than in SU-IVF group. DT selection yielded spermatozoa with low RCDs, DFI, ASA, and caspase-3 which would be benefit for ART.

Influence of bovine sperm DNA fragmentation and oxidative stress on early embryo in vitro development outcome

Reproduction ◽  
2013 ◽  
Vol 146 (5) ◽  
pp. 433-441 ◽  
Author(s):  
Renata Simões ◽  
Weber Beringui Feitosa ◽  
Adriano Felipe Perez Siqueira ◽  
Marcilio Nichi ◽  
Fabíola Freitas Paula-Lopes ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Fragmentation ◽  
Sperm Chromatin ◽  
Dna Integrity ◽  
Sperm Dna Fragmentation ◽  
Cleavage Rate ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Significant Difference

Sperm chromatin fragmentation may be caused by a number of factors, the most significant of which is reactive oxygen species. However, little is known about the effect of sperm oxidative stress (OS) on DNA integrity, fertilization, and embryonic development in cattle. Therefore, the goal of this study was to evaluate the influence of sperm OS susceptibility on the DNA fragmentation rate and in vitro embryo production (IVP) in a population of bulls. Groups of cryopreserved sperm samples were divided into four groups, based on their susceptibility to OS (G1, low OS; G2, average OS; G3, high OS; and G4, highest OS). Our results demonstrated that the sperm DNA integrity was compromised in response to increased OS susceptibility. Furthermore, semen samples with lower susceptibility to OS were also less susceptible to DNA damage (G1, 4.06%; G2, 6.09%; G3, 6.19%; and G4, 6.20%). In addition, embryo IVP provided evidence that the embryo cleavage rate decreased as the OS increased (G1, 70.18%; G2, 62.24%; G3, 55.85%; and G4, 50.93%), but no significant difference in the blastocyst rate or the number of blastomeres was observed among the groups. The groups with greater sensitivity to OS were also associated with a greater percentage of apoptotic cells (G1, 2.6%; G2, 2.76%; G3, 5.59%; and G4, 4.49%). In conclusion, we demonstrated that an increased susceptibility to OS compromises sperm DNA integrity and consequently reduces embryo quality.

Pregnany outcome after IVF/ET among couples who have elevated DNA fragmentation determined by the sperm DNA integrity assay (SDIa™)

2003 ◽  
Vol 80 ◽  
pp. 207
Author(s):  
Carolyn B. Coulam ◽  
Peter Alhering ◽  
Herman Rodriguez ◽  
Levent Keskintepe ◽  
Geoffrey Sher ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Dna Integrity ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Ivf Et

scholarly journals Can oocytes repair fragmented DNA of spermatozoa?

2020 ◽  
Vol 8 (2) ◽  
pp. 73-77
Author(s):  
Michal Ješeta ◽  
Markéta Myšková ◽  
Jana Žáková ◽  
Igor Crha ◽  
Karel Crha ◽  
...  
Keyword(s):  
Sperm Cell ◽  
Sperm Count ◽  
External Factors ◽  
Dna Integrity ◽  
Repair System ◽  
Male Factor ◽  
Sperm Dna Integrity ◽  
Fragmented Dna ◽  
Sperm Dna

AbstractApproximately half of the cases of infertility are due to male factor. In many cases the underlying cause of male infertility is not discovered and, therefore, the condition is considered idiopathic. Examination of morphology, motility, concentration and total sperm count is very important but not sufficient for complex men fertility evaluation. Sperm DNA integrity is a very important one. Sperm DNA can be fragmented by several internal or external factors. In immature sperm cells, the DNA can be repaired by reparatory mechanisms of spermatogonia or spermatocytes. However, in a haploid mature sperm cell, these fragments can not be repaired by male and the fragmented DNA is transferred to oocyte. Oocytes are able to repair male fragmented DNA after their fertilization. A quality embryo can repair damaged sperm DNA and the repair system depends on cytoplasmic and genomic quality of the oocyte. The ability of oocyte to repair sperm DNA strong depend on quality of fertilized oocytes.Running title: Oocyte and DNA repair

scholarly journals Association Study of Sperm DNA Integrity, Routine Semen Parameters and In Vitro Fertilization Clinical Outcome

2021 ◽  
Author(s):  
Yunzhu Lan ◽  
Xinjian Feng ◽  
Xingyu Sun ◽  
Li Fu
Keyword(s):  
In Vitro Fertilization ◽  
Clinical Outcome ◽  
Semen Parameters ◽  
Dna Integrity ◽  
Cleavage Rate ◽  
Vitro Fertilization ◽  
Progressive Motility ◽  
Sperm Dna Integrity ◽  
Sperm Dna

Abstract This paper explores the relationship among sperm DNA integrity, routine semen parameters and in vitro fertilization (IVF) clinical outcome. It applies sperm chromatin dispersion (SCD) test to conduct sperm DNA fragmentation index (DFI) towards the semen samples of 60 male patients who undergoing assisted reproduction techniques (ART) treatments including intrauterine insemination (IUI), in vitro fertilization (IVF), and intracytoplasmic sperm injection (ICSI) treatment in human assisted reproductive medical technology of our hospital. According to the test results, semen samples are divided into group A (DFI≤10%), group B (10%<DFI<30%) and group C (DFI≥30%) to compare semen routine main parameters in each group as well as fertility rate, cleavage rate and high-quality embryonic rate. There is a negative correlation among DFI and sperm activate rate, progressive motility(PR) rate as well as non-progressive motility(NP) rate. The difference of the sperm activate rate, progressive motility rate and non-progressive motility rate in three groups are statistically significant (P<0.05), and there is no statistically significant difference in sperm concentration (P>0.05). The fertility rate, cleavage rate and high-quality embryonic rate in each group have no statistical significance (P>0.05). Sperm DNA integrity have some connection with routine semen parameters, thus, it could play a guiding role for the analysis of semen routine diagnosis. DFI has little influence on the assisted reproductive technology IVF clinical outcome, which requires follow-up tracking clinical outcome and a large number of samples for validation.

scholarly journals Effect of long-term storage in Safe Cell+ extender on boar sperm DNA integrity and other key sperm parameters

2017 ◽  
Vol 59 (1) ◽  
Author(s):  
Wiesław Bielas ◽  
Wojciech Niżański ◽  
Agnieszka Partyka ◽  
Anna Rząsa ◽  
Ryszard Mordak
Keyword(s):  
Sperm Parameters ◽  
Dna Integrity ◽  
Long Term Storage ◽  
Sperm Dna Integrity ◽  
Sperm Dna ◽  
Boar Sperm ◽  
Term Storage
Sign in / Sign up

Export Citation Format

Share Document