scholarly journals ELECTRON MICROSCOPIC LOCALIZATION OF ACID PHOSPHATASE IN RAT THYROID FOLLICLE CELLS AFTER STIMULATION WITH THYROTROPIC HORMONE

1965 ◽  
Vol 13 (8) ◽  
pp. 687-690 ◽  
Author(s):  
ROLF SELJELID
Keyword(s):  
Acid Phosphatase ◽  
Follicle Cells ◽  
Thyroid Follicle ◽  
Electron Microscopic ◽  
Thyrotropic Hormone ◽  
Microscopic Localization ◽  
Rat Thyroid ◽  
Electron Microscopic Localization

Pollen-Wall Proteins: Electron-Microscopic Localization of Acid Phosphatase in the Intine of Crocus Vernus

1971 ◽  
Vol 8 (3) ◽  
pp. 727-733
Author(s):  
R. B. KNOX ◽  
J. HESLOP-HARRISON
Keyword(s):  
Electron Microscope ◽  
Acid Phosphatase ◽  
Coupling Reaction ◽  
Pollen Grain ◽  
Microscope Level ◽  
Electron Microscopic ◽  
Electron Microscope Level ◽  
Microscopic Localization ◽  
Filamentous Inclusions ◽  
Electron Microscopic Localization

Acid phosphatase has been localized in the wall of the pollen grain of Crocus vernus Wulf at the electron-microscope level by a method using 2-naphthyl thiol phosphate as substrate in a simultaneous coupling reaction with fast blue BBN at pH 5.0, the product being given electron opacity by osmication. Activity was found to be concentrated mainly in the intine, and to be associated with ribbon-like or filamentous inclusions believed to be proteinaceous on the basis of other criteria. Some activity was also detectable in the interstices of the exine. The observations confirm the general interpretation of the distribution of wall-held enzyme based upon light-microscopic cytochemistry, and provide the resolution necessary to establish unambiguously that they are associated with protein layers inserted during intine growth.

scholarly journals THE LOCALIZATION OF ARYL SULFATASE ACTIVITY IN THYROID FOLLICLE CELLS

1968 ◽  
Vol 16 (7) ◽  
pp. 467-472 ◽  
Author(s):  
ROLF SELJELID ◽  
HEIKKI J. HELMINEN
Keyword(s):  
Male Rats ◽  
Follicle Cells ◽  
Electron Microscopic Level ◽  
Thyroid Follicle ◽  
Thyroid Activity ◽  
Electron Microscopic ◽  
Thyrotropic Hormone ◽  
Prolonged Incubation ◽  
Aryl Sulfatase ◽  
Sulfatase Activity

The localization of aryl sulfatase activity in thyroid follicle cells was studied with cytochemical techniques both on the light and the electron microscopic level. Twenty male rats received thyroxine to suppress the thyroid activity. To 10 of these animals, thyrotropic hormone (TSH) was given 15-140 min before sacrifice. In both experimental groups, there was dense precipitation indicating aryl sulfatase activity in the cytosomes. In the TSH-stimulated group colloid droplets were observed. These did not show aryl sulfatase activity, not even after prolonged incubation. The number of enzyme-containing bodies in the cytoplasm seemed to decrease after TSH-stimulation, probably because of fusion of colloid droplets with preexisting cytosomes. The lack of enzyme activity in colloid droplets is discussed and some possible explanations are offered.

The electron microscopic localization of nephrotoxic antibodies in isolated glomeruli

Pathology ◽  
1976 ◽  
Vol 8 (1) ◽  
pp. 73-80 ◽  
Author(s):  
I.P. McCausland ◽  
R.N. Seelye ◽  
J.B. Gavin ◽  
P.B. Herdson
Keyword(s):  
Electron Microscopic ◽  
Isolated Glomeruli ◽  
Microscopic Localization ◽  
Electron Microscopic Localization
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