scholarly journals MICROSCOPIC IMMUNOFLUORESCENCE OF GLOMERULI IN CHRONIC NEPHROTOXIC SERUM NEPHRITIS

1965 ◽  
Vol 13 (5) ◽  
pp. 350-354 ◽  
Author(s):  
J. H. BOSS

The microscopic immunofluorescence of the glomeruli differs in acute and chronic nephrotoxic serum nephritis in the distribution pattern of the injected nephrotoxic globulin and the host's gamma globulin. In acute nephritis specific glomerular fluorescence, denoting fixation of nephrotoxic and recipient's gamma globulin, is sharply limited to regular, thin and delicate lines corresponding to the capillary basement membranes. On the other hand, specific glomerular fluorescence in chronic nephritis appears as irregular, broad and tortuous loops consisting of coarse, partially coalescing specks of differing brightness. This difference is possibly related to the profound alterations in chronic nephritis resulting in reconstruction of the glomerular architecture.

1964 ◽  
Vol 119 (6) ◽  
pp. 965-982 ◽  
Author(s):  
Emil Unanue ◽  
Frank J. Dixon

Rats with nephrotoxic serum nephritis may show fixation of complement (C') in the glomeruli during two periods. The first period occurs immediately after the injection of the antisera and extends until the host response occurs. The second period is related to the deposit of rat gamma globulin in the glomeruli presumably occurring at the time of the immune response to the heterologous nephrotoxic serum (NTS). This second period terminates about 2 to 3 weeks after injection of NTS, presumably at the time the rat stops making antibodies to the nephrotoxic gamma globulin fixed in the glomeruli. After this period, the glomerular lesion is incapable of fixing detectable amounts of circulating C' unless the antibody response to the heterologous globulin is reactivated. The immunological events which take place in the initial 2 to 3 week period appear to produce a permanent and irreparable lesion in the glomeruli leading to chronic nephritis. Rabbit NTS produces fixation of rat C' during both periods. Duck NTS shows no detectable fixation of rat C' until rat gamma globulin fixes in the glomeruli at the time of the host immune response to the heterologous nephrotoxic gamma globulin. Decomplementation of rats injected with rabbit NTS produces an amelioration of the initial period of nephrotoxic serum nephritis. The fixation of C' in the glomerular lesions was studied with the use of fluorescent antibody methods. The detection of host beta-1C globulin and gamma globulin in the glomerulus pointed to an immune reaction but not necessarily an active one. The tissue C' fixation test using kidney slices and guinea pig C' proved to be a more sensitive method of detecting tissue reactants capable of fixing C', but its relationship to in vivo events is not certain.


1965 ◽  
Vol 111 (479) ◽  
pp. 999-1002 ◽  
Author(s):  
R. D. Eastham ◽  
J. Jancar ◽  
Ethel H. L. Duncan

Increase in serum gamma globulin concentration with reciprocal reduction in serum albumin concentration is commonly found in patients suffering from Down's syndrome (mongolism) (Carver et al., 1959; Nelson, 1961; Pritham et al., 1963). It is possible that gamma globulin of relatively less effective composition than normal is produced, and that hospitalized living increases production of this faulty protein (Pritham et al., 1963). On the other hand, Polani (1962) suggested that in Down's syndrome there is either exaggerated or diminished response to drugs and hormones, and the raised gamma globulin levels could result from an excessive production of normal gamma globulin, possibly in response to infection.


1963 ◽  
Vol 117 (6) ◽  
pp. 1019-1034 ◽  
Author(s):  
Dietrich K. Hammer ◽  
Frank J. Dixon

The primary phase of nephrotoxic serum nephritis produced by rabbit nephrotoxic serum appears to be dependent to a great extent, but not completely, upon the participation of serum complement. On the other hand, duck nephrotoxic serum produces its primary renal injury without detectable utilization of or dependence upon serum complement. The secondary phase of nephrotoxic serum nephritis appears to be largely or entirely dependent upon the host's antibody response to the heterologous gamma globulin fixed in the glomeruli. No evidence could be obtained for the existence of an autoimmune antikidney response by the host in this experimental model.


1961 ◽  
Vol 7 (2) ◽  
pp. 163-167 ◽  
Author(s):  
V. E. Henderson ◽  
H. Katznelson

The number of nematodes in the rhizosphere of wheat, barley, oats, soybeans, and peas is greater than in the adjacent root-free soil. Pratylenchus sp. is present in the rhizosphere of grains in greater numbers than in the rhizosphere of legumes; Paratylenchus sp. on the other hand showed a preference for legumes. Other genera identified were more abundant in the rhizosphere than in root-free soil, but were not as numerous as Pratylenchus or Paratylenchus, nor did they show a preference for any of the plants tested. One nematode species (Tylenchorhynchus) was present in small numbers and was equally abundant in the rhizosphere and in root-free soil. The consistent distribution pattern of nematodes in the rhizosphere of wheat and of soybeans, observed at high levels of illumination (1200 foot-candles), was not maintained when the illumination was reduced to 300 ft-c or less.


PEDIATRICS ◽  
1964 ◽  
Vol 33 (3) ◽  
pp. 466-466
Author(s):  
DONALD E. CAREY

The conclusions of Amer et al. (Pediatrics 32:4, July, 1963) associating benefits with the administration of gamma-globulin to premature infants are provocative. I was bothered, however, as was the commentator (Dr. Hodes) by the "disproportionate loss of participants between the two groups," 45 from the gamma-globulin groups and 68 from the controls. The authors state that there was no evidence these losses biased the final samples. If, on the other hand, the populations actually were comparable, one would have expected the chances of a family dropping out of either group to be equal. This would have meant about 56 dropouts from each group.


2021 ◽  
Vol 68 (1) ◽  
pp. 179-188
Author(s):  
Shodo Mtow ◽  
Tadaaki Tsutsumi

The first instar nymphs of two peltoperlid stoneflies, i.e., Microperla brevicauda Kawai, 1958 of Microperlinae and Yoraperla uenoi (Kohno, 1946) of Peltoperlinae, were examined and described. Additionally, the phylogeny and groundplan of the first instar nymphs of Peltoperlidae and Plecoptera were considered. The first instar nymphs of M. brevicauda have a slender body with a prognathous head of typical shape; they represent a groundplan in Plecoptera. On the other hand, the first instar nymphs of Y. uenoi have a broad, cockroach-like body with an orthognathous and shortened head, the latter being regarded as a potential autapomorphy of Peltoperlinae. Such differences in body shape between the subfamilies are speculated to arise from heterochrony. The three-segmented cerci of Y. uenoi are characteristic to Systellognatha, whereas the four-segmented cerci of M. brevicauda were independently acquired within Microperlinae. The structure and distribution pattern of chloride cells in the first instar nymphs of Plecoptera were also discussed. The presence of coniform chloride cells is a potential groundplan of Arctoperlaria. One to two pairs of chloride cells are distributed on the first nine abdominal segments of M. brevicauda; this represents a groundplan character of Systellognatha. On the other hand, one to four pairs of chloride cells are found on the second to ninth abdominal segments of Y. uenoi; this distribution pattern may be an apomorphic groundplan of Peltoperlinae.


1951 ◽  
Vol 94 (3) ◽  
pp. 213-221 ◽  
Author(s):  
Alexander S. Wiener

By following the titer of passively acquired Rh antibodies in newborn infants, it is calculated that the half-life of the placenta-passing Rh antibody molecule is approximately 30 days. Evidence is offered that this estimate applies not only to placenta-passing Rh antibodies, but also to other placenta-passing antibodies, and not improbably to serum gamma globulin molecules in general. Studies on the fate of free Rh antibodies in erythroblastotic babies show that these antibodies often are eliminated no more rapidly in Rh-positive babies than in Rh-negative babies. On the other hand, incompatible alpha and beta antibodies, as a rule, are quickly neutralized and eliminated from the body of newborn infants. These observations provide one explanation for the lower incidence of erythroblastosis due to A-B sensitization in comparison with cases resulting from Rh sensitization.


1965 ◽  
Vol 121 (3) ◽  
pp. 323-338 ◽  
Author(s):  
Hans L. Spiegelberg ◽  
William O. Weigle

The catabolism of homologous and heterologous 7S gamma globulin fragments obtained by pepsin and papain digestion was studied in rabbits, guinea pigs, and mice. The elimination from the circulation of I* labeled gamma globulin fragments was followed and the urinary excretion of the total and protein-bound I* activity determined. Evidence is presented that the molecular structure responsible for the catabolism of 7S gamma globulin is located in papain fragment III. The elimination of papain fragment III was slow and closely related to the intact gamma globulin, whereas the pepsin fragment and papain fragments I and II were rapidly eliminated and catabolized in all species examined. Prolonged incubation with cysteine altered papain fragment III as shown by a rapid catabolism of a large portion of incubated fragment III within 24 hours after injection. Small amounts of intact RGG and RGG papain fragment III were excreted as protein-bound I* activity in the urine. On the other hand, large amounts of the pepsin fragment and papain fragments I and II of RGG were excreted as protein-bound I* activity in the urine. The possibility of a molecular structure present in papain fragment III, which may be responsible for tubular reabsorption in the kidney, is discussed. The rate of urinary excretion of fragments obtained from RGG was different from that of fragments obtained from gamma globulin of several other species. In general, small amounts of the pepsin fragment and papain fragment III obtained from gamma globulin other than RGG were excreted as protein-bound I* activity. The amounts of fragment I* excreted as protein-bound I* activity depended on the species in which it was injected, as well as the source of the gamma globulin. The rapid catabolism of the pepsin fragment and papain fragments I or II which bear antibody-combining sites suggest that their use for the prophylactic treatment of tetanus and diphtheria in man is limited.


2019 ◽  
Vol 1 ◽  
pp. 1-2
Author(s):  
Kohei Okamoto ◽  
Masatoshi Morita

<p><strong>Abstract.</strong> The Great East Japan Earthquake in 2011 made a realization of non-Japanese living dispersed in various areas across Japan. The non-Japanese trainees working in the small-size factories in the underpopulated areas and the immigrant wives married to Japanese men in the farming areas were there to compensate for the decreasing Japanese working-age population and the decreasing number of Japanese women who will marry farmers. These women, mostly Chinese and Filipino, live in local communities, isolated from the other non-Japanese residents; they cannot use their mother tongue, they cannot get mutual helps, and they cannot form ethnic community. Their distribution pattern is very different from that in foreigner-concentrated areas in metropolises like Tokyo and Osaka. Ethnicity studies in geography and sociology have mainly focused on foreigner-concentrated areas. There have been few studies on ethnic minorities who live dispersed among their host society. They have been invisible in Japanese society. This study try to clarify where in Japan non-Japanese are living dispersed and develop the method to measure how sparsely they are scattered.</p><p>This study analyses the distribution pattern on non-Japanese by using the Grid Square Statistics of 2010 Population Census and GIS. The Grid Square Statistics is one of the small area statistics which divides the whole area of Japan into small mesh. This study uses statistics of 1&amp;thinsp;km&amp;thinsp;&amp;times;&amp;thinsp;1&amp;thinsp;km grids (Figure 1).</p><p>So far, we have seen that the degree of the isolation of the non-Japanese woman is remarkable in north-eastern Japan. For example, among the 3,249 inhabited grids of Yamagata Prefecture, 1,291 of which had non-Japanese residents, 509 had only one non-Japanese, and 479 of those 509 non-Japanese were women. In other words, 15.7% of the one-square-kilometre inhabited grids in Yamagata Prefecture had only one non-Japanese resident and that person was female, which is the highest among 47 prefectures in Japan. On the other hand, this ratio was relatively low in the metropolitan areas including Kanagawa prefecture and the prefectures of southwestern Japan (Figure 2).</p><p>This study identifies the grid which have only one non-Japanese and that person is female as “isolated-grid”. In the isolated-grids, there are grids where no foreigners live in the 8 adjacent grids, which could be named as “more-isolated-grid” (Figure 1). When we calculate the proportions of the grids for each prefecture and draw them with Quantile classification (Figure 3, left), isolated-grids are distributed in eastern Japan such as Tohoku region same as Figure 2. On the other hand, more-isolated-grids are found not only in eastern Japan but also in western Japan such as Kyushu (Figure 3, right). This is a new finding that has never been said before.</p>


1999 ◽  
Vol 173 ◽  
pp. 249-254
Author(s):  
A.M. Silva ◽  
R.D. Miró

AbstractWe have developed a model for theH2OandOHevolution in a comet outburst, assuming that together with the gas, a distribution of icy grains is ejected. With an initial mass of icy grains of 108kg released, theH2OandOHproductions are increased up to a factor two, and the growth curves change drastically in the first two days. The model is applied to eruptions detected in theOHradio monitorings and fits well with the slow variations in the flux. On the other hand, several events of short duration appear, consisting of a sudden rise ofOHflux, followed by a sudden decay on the second day. These apparent short bursts are frequently found as precursors of a more durable eruption. We suggest that both of them are part of a unique eruption, and that the sudden decay is due to collisions that de-excite theOHmaser, when it reaches the Cometopause region located at 1.35 × 105kmfrom the nucleus.


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