scholarly journals THE COMBINED USE OF MICROINCINERATION AND THE PRUSSIAN BLUE REACTION FOR A MORE SENSITIVE HISTOCHEMICAL DEMONSTRATION OF IRON

1964 ◽  
Vol 12 (3) ◽  
pp. 153-155 ◽  
Author(s):  
R. H. FENTON ◽  
F. B. JOHNSON ◽  
L. E. ZIMMERMAN
Keyword(s):  
Prussian Blue ◽  
Histochemical Demonstration ◽  
Combined Use ◽  
Blue Reaction

scholarly journals THE ACCUMULATION OF IRON IN TUBERCULOUS AREAS

1931 ◽  
Vol 53 (6) ◽  
pp. 919-927 ◽  
Author(s):  
Valy Menkin ◽  
Miriam F. Menkin
Keyword(s):  
Prussian Blue ◽  
Ferric Chloride ◽  
Lung Tissue ◽  
Iron Content ◽  
Chloride Solution ◽  
Ferric Chloride Solution ◽  
Intravenous Injections ◽  
Blue Reaction

Repeated daily intravenous injections of ferric chloride solution are followed by an accumulation of iron in tuberculous areas of lungs. The iron accumulates in the caseous areas of tubercles and is demonstrable by the Prussian blue reaction. Quantitative determinations corroborate these results and show that the iron content of lung tissue in tuberculous animals injected with ferric chloride exceeds that in normal animals injected with this salt, as well as that in non-injected tuberculous animals.

scholarly journals Spectrophotometric determination of ascorbic acid in grapes with the Prussian Blue reaction

2012 ◽  
Vol 23 (2) ◽  
pp. 174-179 ◽  
Author(s):  
Nicoleta Matei ◽  
Simona Dobrinas ◽  
Gabriel Lucian Radu
Keyword(s):  
Ascorbic Acid ◽  
Vitamin C ◽  
Vitis Vinifera ◽  
Prussian Blue ◽  
Spectrophotometric Determination ◽  
Pinot Noir ◽  
Cabernet Sauvignon ◽  
Red Grapes ◽  
Blue Reaction

AbstractThe objective of the present work was to adapt the Prussian Blue reaction for the determination of ascorbic acid. The procedure was successfully applied for the determination of ascorbic acid in red and white grapes (Vitis vinifera L.) just previous ingathering. In the present work was used the red and white grapes from Murfatlar vineyard: Mamaia, Cabernet Sauvignon, Merlot, Pinot Noir, Chardonnay, Sauvignon, Muscat Ottonel and Riesling Italian. The results were situated in the range of 0.67 - 1.79 mg vitamin C/100g product for red grapes and respectively 0.50 - 1.49 mg vitamin C/100g for white grapes.

scholarly journals The Application of the Prussian Blue Stain to Previously Stained Films of Blood and Bone Marrow

Blood ◽  
1955 ◽  
Vol 10 (2) ◽  
pp. 160-166 ◽  
Author(s):  
R. DOROTHY SUNDBERG ◽  
HARRIETTE BROMAN
Keyword(s):  
Bone Marrow ◽  
Prussian Blue ◽  
Formalin Fixation ◽  
Soluble Form ◽  
Simple Method ◽  
Green Material ◽  
Green Color ◽  
Particulate Iron ◽  
Blue Stain ◽  
Blue Reaction

Abstract A simple method for staining non-hemoglobin iron in erythrocytes, normoblasts, macrophages, and other cells containing particulate iron in new or old films of cellular fluids or imprints of tissues and organs is presented. This method consists in using the prussian blue reagent as a type of counterstain; no separate decolorization is necessary. The preparations obtained resemble the original preparations except that iron stands out as a vivid blue-green material. The method is particularly useful in studying conditions accompanied by varying degrees of iron excess or hemosiderosis of the marrow. The stainable iron is all virtually the same color. The diffuse blue-green color of the cytoplasm of macrophages might be attributed to the more soluble form of iron, ferritin. Stainable iron is visible in normoblasts and erythrocytes as well as in macrophages in sections subjected to the prussian blue reaction. A prussian blue method using formalin fixation on fresh films of marrow7 has also been shown to be useful in the demonstration of particulate iron in previously stained films of marrow and blood. The formalin fixation appears to bring about a higher percentage of siderocytes.

scholarly journals 379 Biochemical Barriers Induced by UV Light and Botrytis cinerea in Postharvest Tomato

HortScience ◽  
1999 ◽  
Vol 34 (3) ◽  
pp. 509C-509
Author(s):  
Marie-Therese Charles ◽  
Alain Goulet ◽  
Francois Castaigne ◽  
Joseph Arul
Keyword(s):  
Cell Wall ◽  
Disease Resistance ◽  
Phenolic Compounds ◽  
Prussian Blue ◽  
Ultraviolet Light ◽  
Uv Light ◽  
Tomato Fruit ◽  
Induced Disease Resistance ◽  
Cell Layers ◽  
Blue Reaction

Hormic dose of ultraviolet light (3.7 kJ•m-2) induced disease resistance in tomato fruit. The biochemical nature of induced resistance by UV light was investigated by histochemical techniques. Ultraviolet light induced plasmolysis of the epicarp and few mesocarp cell layers, and collapse of these cell layers led to the formation of cell wall stacking zone (CWSZ). The treatment also stimulated the biosynthesis of phenolic compounds (Prussian blue reaction) in the epicarp and mesocarp cells. Biochemical reinforcement of the cell wall through lignification (Maule test) and suberization (berberine fluorescence) was also induced. These responses originating from the activation of phenylpropanoid path were principally localized in the CWSZ and were induced before inoculation by B. cinerea. The intensity of these responses was significantly increased in UV-treated tissue in response to infection. These responses were also induced in the inoculated control tissue but were either less substantial (phenolics, lignification, and suberization) or delayed.

An Apparently Unpublished Source of Precipitates in the Prussian Blue Reaction for Iron

1964 ◽  
Vol 42 (6_ts) ◽  
pp. 640-641
Author(s):  
Elizabeth H. Snitzer ◽  
Faye Sweat ◽  
B. Geneva S. Gray
Keyword(s):  
Prussian Blue ◽  
Blue Reaction

scholarly journals Ferrocyanide staining of transferrin and ferritin-conjugated antibody to transferrin.

1979 ◽  
Vol 27 (2) ◽  
pp. 681-685 ◽  
Author(s):  
R T Parmley ◽  
F Ostroy ◽  
R A Gams ◽  
L DeLucas
Keyword(s):  
Cell Surface ◽  
Prussian Blue ◽  
Tumor Cells ◽  
Ferric Iron ◽  
Kinetic Studies ◽  
Ascites Tumor ◽  
Preliminary Results ◽  
Blue Reaction

To evaluate the ultrastructural distribution of transferrin on the surface of L1210 ascites tumor cells, we used ferrocyanide to stain ferric iron (Prussian blue reaction) in transferrin, as well as in ferritin conjugated to antibody that was immunologically attached to the transferrin. Small deposits averaging 5 nm in diameter identified transferrin iron, whereas large cuboidal deposits averaging 50 nm in diameter stained ferritin conjugated-antibody that was bound to both transferrin and apotransferrin on the cell surface. The ability of transferrin to deliver iron to ascites tumor cells was confirmed by kinetic studies of transferrin labeled with 59Fe and 125I. These preliminary results are consistent with release of transferrin iron at the cell surface and demonstrate additional uses for ferrocyanide in ultrastructural cytochemical techniques.

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