scholarly journals THE ACTION OF FORMALDEHYDE SOLUTIONS ON HUMAN BRAIN LIPIDS

1962 ◽  
Vol 10 (6) ◽  
pp. 704-709 ◽  
Author(s):  
F. J. M. HESLINGA ◽  
F. A. DEIERKAUF

The composition of the lipids extracted from fresh normal human brain and from brains preserved up to 24 years in unbuffered formaldehyde solutions was analysed quantitatively using column and paper chromatography, followed by various determinations. The amounts of cholesterol, cerebrosides, sulphatides, phosphoinositides and sphingomyelin remain unaffected. Lecithin, phosphatidylethanolamine and phosphatidylserine are broken down to the corresponding lyso compounds, fatty acids, phosphatidic acid and probably lysophosphatidic acid. The lyso compounds are further broken down by the liberation of the second fatty acid. By this process the fatty acid content is markedly increased. The total lipid content decreases slightly by the formation of water soluble phosphoryl compounds. Consequently the phosphorus content of the total lipid extract suffers marked diminution. Histochemical reactions on lipids in formaldehyde stored tissues should preferably be performed in combination with an analysis of the lipid extract.

1975 ◽  
Vol 148 (1) ◽  
pp. 139-144 ◽  
Author(s):  
K M Marriott ◽  
D H Northcote

1. Lipid extracts were obtained from castor-bean endosperm tissue at various times during germination and, after purification, the total lipid content was determined. Quantitative measurements of the triglyceride and phospholipid content together with the fatty acid composition were made. 2. The total lipid content of the endosperm rapidly decreased during germination; after 10 days less than 20% of the original weight of lipid remained. In contrast, the phospholipid content (initially less than 0.5% of the total lipid) increased slightly during this time. The fatty acid composition and the relative proportions of the triglyceride species of the total lipid extract remained constant during 10 days of germination. 3. Gibberellic acid (0.3 mM) markedly stimulated the rate of lipid breakdown but did not alter either the fatty acid composition or the relative proportion of triglyceride species. 4. The embryo had little effect on lipid metabolism in the endosperm tissue; only after 6 days of germination were differences observed in the rate of fat utilization in the presence and absence of the embryo.


1995 ◽  
Vol 75 (2) ◽  
pp. 247-253 ◽  
Author(s):  
D. M. Nash ◽  
R. M. G. Hamilton ◽  
H. W. Hulan

The effect of dietary HM on plasma and egg yolk lipids of commercial White Leghorn hens was studied over 350 d. A total of 192 birds were given corn-wheat-soybean meal diets that contained either 0, 4, 8, or 12% HM. Analysis of plasma lipids at four periods in the laying cycle (169, 211, 253 and 287 d) and at five times during the test day (0800, 1000, 1200, 1400 and 1600 h) were performed. Egg lipids were analyzed at each of the four periods.Plasma total lipids were inversely related (P < 0.01) to dietary HM levels while omega-3 and omega-6 fatty acid levels were positively and inversely related (P < 0.001), respectively. HM levels did not influence the total lipid content of the egg yolk lipids but omega-3 and omega-6 fatty acid content were positively and inversely related, respectively. The levels of eicosapentaenoic (20:5n3, EPA) and docosahexaenoic (22:6n3, DHA) acid were 11 and 3 times higher, respectively (7.8 and 100.5 mg yolk−1) in the yolks from hens given the 12% HM diet compared to the control diet. Key words: Herring meal, laying hens, omega-3 fatty acids, eicosapentaenoic acid, docosahexaenoic acid


2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Volha Shapaval ◽  
Jule Brandenburg ◽  
Johanna Blomqvist ◽  
Valeria Tafintseva ◽  
Volkmar Passoth ◽  
...  

Langmuir ◽  
2019 ◽  
Vol 35 (36) ◽  
pp. 11940-11949 ◽  
Author(s):  
Dusan Mrdenovic ◽  
Marta Majewska ◽  
Izabela S. Pieta ◽  
Piotr Bernatowicz ◽  
Robert Nowakowski ◽  
...  

2011 ◽  
Vol 60 (5) ◽  
pp. 217-220 ◽  
Author(s):  
Yousuke Taoka ◽  
Naoki Nagano ◽  
Yuji Okita ◽  
Hitoshi Izumida ◽  
Shinichi Sugimoto ◽  
...  

Author(s):  
Onkar Singh Brraich ◽  
Navpreet Kaur ◽  
Swarndeep Singh Hundal

Fish production and fish processing waste have straight connection.  In India, the waste produced during the processing of fish is predicted to be approximate 3.6 million metric tonnes, 48 per cent of the total body weight of Indian and exotic major carps is thrown away as waste (non-edible ). The present research, it was conducted to compare the total lipid content (TLC) and fatty acid composition from the liver of captured and cultured fish, Labeo rohita  (Hamilton) having weight more than 500 gram during different months as well as to evaluate its nutritional quality. Maximum total lipid content (33.33±0.14%) was found in the liver of cultured fish in May month, while the minimum (15.26±0.24%) was in the liver of captured fish in the month of January. During the study, total lipid content was found to be considerably elevated in cultured than captured Fish, Labeo rohita  (Hamilton). The amount of three major groups of fatty acids namely polyunsaturated fatty acids, monounsaturated fatty acids and saturated fatty acids was found to be maximum in captured fish during the month of March 97.19±0.96%, 61.30±0.56% and 95.39±0.31% month of April respectively. Total n-6 polyunsaturated fatty acids were observed to be highest (21.95±8.05%) in the waste of liver in cultured fish during the month of March. Hence, it is concluded that the processing waste (liver) of the captured and cultured, Labeo rohita (Hamilton) is a prosperous resource of the essential fatty acids i.e.  PUFAs and total lipids. Further, it is observed that captured species are rich in fatty acid composition as compared to cultured species. Food industries can manufacture by-products from these high nutritional value contents of   waste for human utilization. EPA and DHA also reduce the risk of various life threatening diseases.


2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
Thi Phuong Lan Nguyen ◽  
Van Tuyen Anh Nguyen ◽  
Thi Thanh Trung Do ◽  
Trung Nguyen Quang ◽  
Quoc Long Pham ◽  
...  

The mud crab increases the yield of farming in Southeast Asian countries. Scylla paramamosain, one of four mud crab species belonging to the Scylla genus, is a rich nutrient source during its soft-shell moulting period. In this study, we analysed the total lipid content, fatty acid components, and phospholipid molecular species of the S. paramamosain mud crab. The total lipid content was 1.62 ± 0.08%, which is similar to that of S. serrata previously reported. Twenty-one fatty acids were identified in S. paramamosain. The composition and molecular forms of the phospholipids were identified using high-performance liquid chromatography-high-resolution mass spectrometry. Fifty-four different molecules belonging to six types of phospholipids were identified. Notably, phospholipids were made of fatty acids with C16 : 0; C18 : 0; C20 : 4; C20 : 5; and C22 : 6 main components. The anti-inflammatory and cytotoxic effects of crab lipids and phospholipids were investigated for the first time. The anti-inflammatory activity of the total and polar lipids had IC50 values of 71.5 and 68.6 μg/mL, respectively. The crab polar lipid fraction, which contained phospholipids, also presented high cytotoxic activity toward five cancer cell lines with IC50 values ranging from 85.4 to 95.8 μg/mL.


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