scholarly journals A CHEMICAL AND HISTOCHEMICAL INVESTIGATION OF GLYCOGEN IN RAT LIVER AND PALATE FOLLOWING TREATMENT WITH VARIOUS FIXATIVES AND ETHYLENEDIAMINE TETRAACETIC ACID

1962 ◽  
Vol 10 (3) ◽  
pp. 245-249 ◽  
Author(s):  
J. R. TROTT ◽  
S. L. GORENSTEIN ◽  
M. D. PEIKOFF
Keyword(s):  
Acetic Acid ◽  
Chemical Analysis ◽  
Periodic Acid ◽  
Tetraacetic Acid ◽  
Fixed Tissue ◽  
Formalin Fixed Tissue ◽  
Ethylenediamine Tetraacetic Acid ◽  
Cell Layers ◽  
Adequate Method ◽  
Formalin Fixed

The loss of glycogen after fixation in acetic acid-alcohol-formalin as compared to immediate determination was found by chemical analysis to be from 5.2% to 13.8%. No appreciable difference could be seen histochemically. Chemical analysis of the glycogen content of liver fixed in acetic acid-alcohol-formalin and then treated in ethylenediamine tetracetic acid showed a total drop of 20.7 to 33.7%. Glycogen could still be adequately shown histochemically. Sections taken from liver fixed in 1% periodic acid in 10% formalin show greater amounts of glycogen, than those taken from acetic-acid-alcohol-formalin fixed tissue. After fixation in 1% periodic acid in 10% formalin and following treatment with ethylenediamine tetraacetic acid, very little glycogen could be demonstrated histochemically. In the palate, no glycogen could be demonstrated after fixation in acetic acid-alcohol-formalin, whereas quite heavy amounts could be seen in the prickle cell layers of the epithelium following fixation in 1% periodic acid in 10% formalin. Neither acetic acid-alcohol-formalin nor 1% periodic acid in 10% formalin are satisfactory fixatives if the tissue is to be further decalcified in ethylenediamine tetraacetic acid, if there are only small amounts of glycogen present. No adequate method of retaining glycogen in small amounts in tissue requiring decalcification can be suggested.

scholarly journals THE EFFECT OF SAPONIFICATION UPON CERTAIN HISTOCHEMICAL REACTIONS OF THE EPITHELIAL MUCINS OF THE GASTROINTESTINAL TRACT

1971 ◽  
Vol 19 (11) ◽  
pp. 654-662 ◽  
Author(s):  
C. F. A. CULLING ◽  
P. E. REID ◽  
W. L. DUNN
Keyword(s):  
Gastrointestinal Tract ◽  
Guinea Pig ◽  
Periodic Acid ◽  
Ileocecal Valve ◽  
Paraffin Sections ◽  
Periodic Acid Schiff ◽  
Fixed Tissue ◽  
Formalin Fixed Tissue ◽  
Cryostat Sections ◽  
Formalin Fixed

An increase in periodic acid-Schiff reactivity after saponification has been demonstrated in the gastrointestinal tract mucins of man, rat, rabbit and guinea pig. Tins pH-dependent phenomenon is independent of fixation and processing since it occurred in cryostat sections of fresh and formalin-fixed tissue and in paraffin sections of tissues fixed in eight different fixatives. In man and rat this effect occurs at the level of the ileocecal valve and below; in guinea pig it is confined to the large intestine and rectum; while in rabbits it occurs in Brunner's glands, the ileocecal valve and below. In man the increase is found throughout the crypts whereas in rabbits the effect is seen mainly at the luminal end; in rats it is seen largely at the base of the crypts; and in guinea pigs it is variable. This effect, due to an increase in the number of 1:2 glycol groups, is accompanied in man, rat and guinea pig by an increase in basophilia due to the presence of sulfate and/or other highly acidic groups.

Sensitivity of Acid-Fast Staining forMycobacterium tuberculosisin Formalin-fixed Tissue

2002 ◽  
Vol 166 (7) ◽  
pp. 994-997 ◽  
Author(s):  
Hajime Fukunaga ◽  
Tomoyuki Murakami ◽  
Toshikazu Gondo ◽  
Kazuo Sugi ◽  
Tokuhiro Ishihara
Keyword(s):  
Fixed Tissue ◽  
Formalin Fixed Tissue ◽  
Formalin Fixed ◽  
Acid Fast Staining

Generation of anti-DC-SIGN monoclonal antibodies capable of blocking HIV-1 gp120 binding and reactive on formalin-fixed tissue

2011 ◽  
Vol 135 (1-2) ◽  
pp. 165-172 ◽  
Author(s):  
Bertrand Canard ◽  
Hortense Vachon ◽  
Thomas Fontaine ◽  
Jean-Jacques Pin ◽  
Stéphane Paul ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Fixed Tissue ◽  
Formalin Fixed Tissue ◽  
Gp120 Binding ◽  
Hiv 1 ◽  
Formalin Fixed

scholarly journals Optimisation of DNA and RNA extraction from archival formalin-fixed tissue

1999 ◽  
Vol 27 (16) ◽  
pp. i-iii ◽  
Author(s):  
N. J. Coombs ◽  
A. C. Gough ◽  
J. N. Primrose
Keyword(s):  
Rna Extraction ◽  
Fixed Tissue ◽  
Formalin Fixed Tissue ◽  
Dna And Rna ◽  
Formalin Fixed

scholarly journals A simple technique for preservation of fixation-sensitive antigens in paraffin-embedded tissues.

1994 ◽  
Vol 42 (8) ◽  
pp. 1127-1134 ◽  
Author(s):  
J H Beckstead
Keyword(s):  
Simple Technique ◽  
Frozen Section ◽  
Lymphoid Tissues ◽  
Frozen Sections ◽  
Morphological Examination ◽  
Fixed Tissue ◽  
Formalin Fixed Tissue ◽  
Important Approach ◽  
Reduced Toxicity ◽  
Formalin Fixed

Immunohistochemistry is a powerful tool for tissue diagnosis and research. Although the frozen section has remained the gold standard for this important approach to evaluation of antigens in tissues, there is widespread acknowledgment of many limitations. Routine paraffin-embedded sections ware widely used for morphological examination of tissues but are not optimal for antigen preservation. In this study, paraffin-embedded tissues fixed with a simple buffer containing zinc as the primary fixative were compared with tissues fixed with routine formalin, zinc-formalin, paraformaldehyde, ethanol, a variety of commercial (non-formalin-containing) fixatives that have been recommended for reduced toxicity and improved antigen survival, and frozen sections. Human lymphoid tissues and a group of antibodies to antigens (CD1, CD4, CD7, CD8, CD19) usually preserved only in frozen tissue were used as a model system. Fixation in a simple solution of zinc acetate and zinc chloride in a Tris-Ca acetate buffer resulted in antigen preservation comparable to that in frozen sections with antibodies to these cell surface markers. Morphological preservation was comparable to formalin-fixed sections. The work presents a new method that represents the closest approach yet to a technique that combines optimal antigenic survival with the convenience and morphological preservation of traditional formalin-fixed tissue embedded in paraffin.

scholarly journals Sequencing of Dna Isolated From Colorectal Sample Fixed in Liquid Formalin : Obstacles and Required Modifications

2020 ◽  
Vol 29 (2) ◽  
pp. 165-174
Author(s):  
Nahid Parvez ◽  
Mustak Ibn Ayub
Keyword(s):  
Dna Isolation ◽  
Low Cost ◽  
General Purpose ◽  
Proteinase K ◽  
Fixed Tissue ◽  
Target Region ◽  
Formalin Fixed Tissue ◽  
Successful Amplification ◽  
Cancer Tissues ◽  
Formalin Fixed

The necessary modifications in the protocol of general purpose DNA isolation kit to isolate and amplify a target region of genome from colorectal cancer tissues fixed in liquid formalin were made. It is shown that a one hour digestion with proteinase K yields enough DNA from formalin fixed colorectal tissue for subsequent PCR and sequencing. Moreover, using 100% ethanol instead of standard 50% during DNA binding step in the column improves the yield. As DNA fragmentation is unavoidable in formalin fixed tissue, PCR protocol was modified by increasing polymerase concentration to get successful amplification. Following these modifications, two regions of KRAS and BRAF genes were amplified and successfully sequenced from three different patients. These modifications provide a low cost option for Sanger sequencing of DNA isolated from formalin fixed tissue. Dhaka Univ. J. Biol. Sci. 29(2): 165-174, 2020 (July)

Improvement of the Quality of Frozen Sections from Formalin fixed Tissue

1990 ◽  
Vol 65 (1) ◽  
pp. 43-44
Author(s):  
Toshihiro Ishii ◽  
Kumiko Kasama ◽  
Mayumi Kondo
Keyword(s):  
Frozen Sections ◽  
Fixed Tissue ◽  
Formalin Fixed Tissue ◽  
Formalin Fixed
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