Bone repair scaffold coated with bone morphogenetic protein-2 for bone regeneration in murine calvarial defect model: Systematic review and quality evaluation

2019 ◽  
Vol 42 (7) ◽  
pp. 325-337 ◽  
Author(s):  
Jian-Hua Zeng ◽  
Peng Qiu ◽  
Long Xiong ◽  
Shi-Wei Liu ◽  
Ling-Hua Ding ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Bone Repair ◽  
Animal Research ◽  
Bone Morphogenetic Protein 2 ◽  
Calvarial Defect ◽  
Research Reporting ◽  
In Vivo Experiments ◽  
Morphogenetic Protein

To systematically assess the effects of hydroxyapatite bone repair scaffold coated with bone morphogenetic protein-2 on murine calvarial defect models and to determine the quality of studies according to the Animal Research Reporting in In Vivo Experiments guidelines. Internet search was performed in duplicate using PubMed, MEDLINE, Ovid and Embase databases (without restrictions on publication date). The Animal Research Reporting in In Vivo Experiments guidelines were used to evaluate the quality of selected studies. Following screening, 12 studies were eligible for the review. Studies with average quality coefficients predominated (66.67%), followed by poor (25%) and excellent (8.33%) quality coefficients. Minimum quality scores were assigned to the Animal Research Reporting in In Vivo Experiments guideline items: housing and husbandry (9), allocation (11), outcomes (12), interpretation (18) and generalizability (19). Sprague–Dawley rats were the most frequently used (50%) species, and most studies had a sample size of more than 30 (58.33%). A defect dimension of 5 mm was the most common (33.33%). The biological hydroxyapatite composite scaffold was common (50%), and the bioactive factors were bone morphogenetic protein-2 (50%) and recombinant human bone morphogenetic protein-2 (50%). Histomorphometric results showed that bone morphogenetic protein-2 enhanced the capacity to regenerate bone considerably. In addition, scaffolds with bone morphogenetic protein-2 resulted in a significant increase in the blood vessel in the new bone. The findings suggested that data on animal experiments of hydroxyapatite scaffold coated with bone morphogenetic protein-2 in murine calvarial defect models lack homogeneity. Animal experiment should follow the Animal Research Reporting in In Vivo Experiments guidelines to promote the high quality, integrity and reproducibility. This systematic review suggested that bone morphogenetic protein-2 enhanced the capacity to regenerate bone and the angiogenesis in the new bone.

scholarly journals The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research*

2020 ◽  
Vol 40 (9) ◽  
pp. 1769-1777 ◽  
Author(s):  
Nathalie Percie du Sert ◽  
Viki Hurst ◽  
Amrita Ahluwalia ◽  
Sabina Alam ◽  
Marc T. Avey ◽  
...  
Keyword(s):  
Animal Research ◽  
Quality Of Reporting ◽  
Minimum Requirement ◽  
Research Reporting ◽  
Journal Editors ◽  
In Vivo Experiments ◽  
Key Concepts ◽  
Delphi Exercise

Reproducible science requires transparent reporting. The ARRIVE guidelines (Animal Research: Reporting of In Vivo Experiments) were originally developed in 2010 to improve the reporting of animal research. They consist of a checklist of information to include in publications describing in vivo experiments to enable others to scrutinise the work adequately, evaluate its methodological rigour, and reproduce the methods and results. Despite considerable levels of endorsement by funders and journals over the years, adherence to the guidelines has been inconsistent, and the anticipated improvements in the quality of reporting in animal research publications have not been achieved. Here, we introduce ARRIVE 2.0. The guidelines have been updated and information reorganised to facilitate their use in practice. We used a Delphi exercise to prioritise and divide the items of the guidelines into 2 sets, the “ARRIVE Essential 10,” which constitutes the minimum requirement, and the “Recommended Set,” which describes the research context. This division facilitates improved reporting of animal research by supporting a stepwise approach to implementation. This helps journal editors and reviewers verify that the most important items are being reported in manuscripts. We have also developed the accompanying Explanation and Elaboration document, which serves (1) to explain the rationale behind each item in the guidelines, (2) to clarify key concepts, and (3) to provide illustrative examples. We aim, through these changes, to help ensure that researchers, reviewers, and journal editors are better equipped to improve the rigour and transparency of the scientific process and thus reproducibility.

scholarly journals A Comparative Genomic and Transcriptional Survey Providing Novel Insights into Bone Morphogenetic Protein 2 (bmp2) in Fishes

2019 ◽  
Vol 20 (24) ◽  
pp. 6137 ◽  
Author(s):  
Guang Yang ◽  
Zhendong Qin ◽  
Hongyan Kou ◽  
Rishen Liang ◽  
Lijuan Zhao ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Bone Repair ◽  
Fish Bone ◽  
Transcriptomic Analysis ◽  
Bone Morphogenetic Protein 2 ◽  
Comparative Genomic ◽  
Sequence Alignments ◽  
Cell Proliferation And Differentiation ◽  
Morphogenetic Protein

Intermuscular bones (IBs) are only found in the muscles of fish. Bone morphogenetic protein 2 (bmp2) is considered to be the most active single osteogenesis factor. It promotes cell proliferation and differentiation during bone repair, as well as inducing the formation of bones and cartilages in vivo. However, detailed investigations of this family in fish are incredibly limited. Here, we have used a variety of published and unpublished bmp2 sequences for teleosts and cartilage fish in order to explore and expand our understanding of bmp2 genes in fish. Our results confirmed that teleost genomes contain two or more bmp2 genes, and the diversity of bmp2 genes in vertebrates appears to be as a result of a combination of whole genome duplication (WGD) and gene loss. Differences were also observed in tissue distribution and relative transcription abundance of the bmp2s through a transcriptomic analysis. Our data also indicated that bmp2b may play an important role in the formation of IBs in teleosts. In addition, protein sequence alignments and 3D structural predictions of bmp2a and bmp2b supported their similar roles in fishes. To summarize, our existing work provided novel insights into the bmp2 family genes in fishes through a mixture of comparative genomic and transcriptomic analysis.

scholarly journals The ARRIVE guidelines 2.0: updated guidelines for reporting animal research

2020 ◽  
Vol 4 (1) ◽  
pp. e100115 ◽  
Author(s):  
Nathalie Percie du Sert ◽  
Viki Hurst ◽  
Amrita Ahluwalia ◽  
Sabina Alam ◽  
Marc T Avey ◽  
...  
Keyword(s):  
Animal Research ◽  
Quality Of Reporting ◽  
Minimum Requirement ◽  
Research Reporting ◽  
Journal Editors ◽  
In Vivo Experiments ◽  
Key Concepts ◽  
Delphi Exercise

Reproducible science requires transparent reporting. The ARRIVE guidelines (Animal Research: Reporting of In Vivo Experiments) were originally developed in 2010 to improve the reporting of animal research. They consist of a checklist of information to include in publications describing in vivo experiments to enable others to scrutinise the work adequately, evaluate its methodological rigour and reproduce the methods and results. Despite considerable levels of endorsement by funders and journals over the years, adherence to the guidelines has been inconsistent, and the anticipated improvements in the quality of reporting in animal research publications have not been achieved. Here, we introduce ARRIVE 2.0. The guidelines have been updated and information reorganised to facilitate their use in practice. We used a Delphi exercise to prioritise and divide the items of the guidelines into two sets, the ‘ARRIVE Essential 10’, which constitutes the minimum requirement, and the ‘Recommended Set’, which describes the research context. This division facilitates improved reporting of animal research by supporting a stepwise approach to implementation. This helps journal editors and reviewers verify that the most important items are being reported in manuscripts. We have also developed the accompanying Explanation and Elaboration document, which serves (1) to explain the rationale behind each item in the guidelines, (2) to clarify key concepts and (3) to provide illustrative examples. We aim, through these changes, to help ensure that researchers, reviewers and journal editors are better equipped to improve the rigour and transparency of the scientific process and thus reproducibility.

scholarly journals A new biocompatible delivery scaffold containing heparin and bone morphogenetic protein 2

2016 ◽  
Vol 66 (3) ◽  
pp. 373-385 ◽  
Author(s):  
Suphannee Thanyaphoo ◽  
Jasadee Kaewsrichan
Keyword(s):  
Tissue Engineering ◽  
Bone Tissue Engineering ◽  
Bone Morphogenetic Protein ◽  
Bone Tissue ◽  
Bone Morphogenetic Protein 2 ◽  
In Vivo Experiments ◽  
Morphogenetic Protein ◽  
Human Bone Morphogenetic Protein

Abstract Silicon-substituted calcium phosphate (Si-CaP) was developed in our laboratory as a biomaterial for delivery in bone tissue engineering. It was fabricated as a 3D-construct of scaffolds using chitosan-trisodium polyphosphate (TPP) cross-linked networks. In this study, heparin was covalently bonded to the residual -NH2 groups of chitosan on the scaffold applying carbodiimide chemistry. Bonded heparin was not leached away from scaffold surfaces upon vigorous washing or extended storage. Recombinant human bone morphogenetic protein 2 (rhBMP-2) was bound to conjugated scaffolds by ionic interactions between the negatively charged SO42- clusters of heparin and positively charged amino acids of rhBMP-2. The resulting scaffolds were inspected for bone regenerative capacity by subcutaneous implanting in rats. Histological observation and mineralization assay were performed after 4 weeks of implantation. Results from both in vitro and in vivo experiments suggest the potential of the developed scaffolds for bone tissue engineering applications in the future.

Endorsement of Animal Research: Incorporation of In Vivo Experiments (ARRIVE) Guidelines/Gold Standard Publication Checklist (GSPC) by Chinese journals: A survey of journals' instructions for authors and editors

2019 ◽  
Vol 54 (5) ◽  
pp. 479-486
Author(s):  
Ting Zhang ◽  
Jingjing Yang ◽  
Xi Bai ◽  
Hongyan Liu ◽  
Fang Cheng ◽  
...  
Keyword(s):  
Gold Standard ◽  
Response Rate ◽  
Recognition Rate ◽  
Animal Research ◽  
Manuscript Preparation ◽  
Quality Of Reporting ◽  
Research Reporting ◽  
In Vivo Experiments

The objective was to determine the rate at which Chinese journals include Animal Research: Reporting of In Vivo Experiments (ARRIVE) Guidelines/Gold Standard Publication Checklist (GSPC) in their instructions for authors, and the awareness and recognition of editors. The survey was performed on Chinese journals. The most recent versions each journal's instructions for authors were downloaded, and the information related to the ARRIVE/GSPC was collected. A self-developed questionnaire was used to conduct the survey among the editors. Questionnaires were sent to 238 qualified journals and 198 of them returned them, achieving an 83.2% response rate. The results showed that none of the journals included the ARRIVE/GSPC in their instructions for authors, and the awareness rate was only 13.1% (26/198). The participants who were unaware of the ARRIVE/GSPC were less likely than those who were aware of them to believe it was necessary to include the ARRIVE/GSPC in the instructions for authors (23.3% vs. 61.5%), and less likely to request authors in their manuscript preparation (28.5% vs. 88.5%), editors in the editing and processing (28.5% vs. 84.6%) and reviewers in peer review stage (28.5% vs. 92.3%) to follow the ARRIVE/GSPC. Currently no Chinese journals include the ARRIVE/GSPC in their instructions for authors. The recognition rate of the ARRIVE/GSPC was low among the editors. So, we suggest that Chinese journals should promote inclusion of the ARRIVE/GSPC in journals' instructions for authors. It is also important to educate researchers and editors alike to increase their understanding of the ARRIVE/GSPC, so that the quality of reporting of animal study can be improved.

scholarly journals In Vivo Host Response and Degradation of Copolymer Scaffolds Functionalized with Nanodiamonds and Bone Morphogenetic Protein 2

2016 ◽  
Vol 5 (6) ◽  
pp. 730-742 ◽  
Author(s):  
Salwa Suliman ◽  
Yang Sun ◽  
Torbjorn O. Pedersen ◽  
Ying Xue ◽  
Joachim Nickel ◽  
...  
Keyword(s):  
Bone Morphogenetic Protein ◽  
Host Response ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein
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