scholarly journals Feline immunodeficiency virus vectors persistently transduce nondividing airway epithelia and correct the cystic fibrosis defect

1999 ◽  
Vol 104 (11) ◽  
pp. R55-R62 ◽  
Author(s):  
Guoshun Wang ◽  
Vladimir Slepushkin ◽  
Joseph Zabner ◽  
Shaf Keshavjee ◽  
Julie C. Johnston ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Feline Immunodeficiency Virus ◽  
Airway Epithelia ◽  
Virus Vectors ◽  
Immunodeficiency Virus

scholarly journals Persistent Gene Expression in Mouse Nasal Epithelia following Feline Immunodeficiency Virus-Based Vector Gene Transfer

2005 ◽  
Vol 79 (20) ◽  
pp. 12818-12827 ◽  
Author(s):  
Patrick L. Sinn ◽  
Erin R. Burnight ◽  
Melissa A. Hickey ◽  
Gary W. Blissard ◽  
Paul B. McCray
Keyword(s):  
Gene Expression ◽  
Gene Transfer ◽  
Epithelial Cells ◽  
Reporter Gene ◽  
Feline Immunodeficiency Virus ◽  
Envelope Protein ◽  
Envelope Glycoproteins ◽  
Airway Epithelia ◽  
Human Airway ◽  
Immunodeficiency Virus

ABSTRACT Gene transfer development for treatment or prevention of cystic fibrosis lung disease has been limited by the inability of vectors to efficiently and persistently transduce airway epithelia. Influenza A is an enveloped virus with natural lung tropism; however, pseudotyping feline immunodeficiency virus (FIV)-based lentiviral vector with the hemagglutinin envelope protein proved unsuccessful. Conversely, pseudotyping FIV with the envelope protein from influenza D (Thogoto virus GP75) resulted in titers of 106 transducing units (TU)/ml and conferred apical entry into well-differentiated human airway epithelial cells. Baculovirus GP64 envelope glycoproteins share sequence identity with influenza D GP75 envelope glycoproteins. Pseudotyping FIV with GP64 from three species of baculovirus resulted in titers of 107 to 109 TU/ml. Of note, GP64 from Autographa californica multicapsid nucleopolyhedrovirus resulted in high-titer FIV preparations (∼109 TU/ml) and conferred apical entry into polarized primary cultures of human airway epithelia. Using a luciferase reporter gene and bioluminescence imaging, we observed persistent gene expression from in vivo gene transfer in the mouse nose with A. californica GP64-pseudotyped FIV (AcGP64-FIV). Longitudinal bioluminescence analysis documented persistent expression in nasal epithelia for ∼1 year without significant decline. According to histological analysis using a LacZ reporter gene, olfactory and respiratory epithelial cells were transduced. In addition, methylcellulose-formulated AcGP64-FIV transduced mouse nasal epithelia with much greater efficiency than similarly formulated vesicular stomatitis virus glycoprotein-pseudotyped FIV. These data suggest that AcGP64-FIV efficiently transduces and persistently expresses a transgene in nasal epithelia in the absence of agents that disrupt the cellular tight junction integrity.

Feline Immunodeficiency Virus Vectors for Efficient Transduction of Primary Human Synoviocytes: Application to an Original Model of Rheumatoid Arthritis

2004 ◽  
Vol 15 (6) ◽  
pp. 588-596 ◽  
Author(s):  
Yea-Lih Lin ◽  
Danièle Noël ◽  
Clément Mettling ◽  
Brigitte Réant ◽  
Jacques Clot ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Feline Immunodeficiency Virus ◽  
Original Model ◽  
Virus Vectors ◽  
Immunodeficiency Virus

Gene Transfer to the Nonhuman Primate Retina with Recombinant Feline Immunodeficiency Virus Vectors

2002 ◽  
Vol 13 (6) ◽  
pp. 689-696 ◽  
Author(s):  
Andrew J. Lotery ◽  
Todd A. Derksen ◽  
Stephen R. Russell ◽  
Robert F. Mullins ◽  
Sybille Sauter ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Nonhuman Primate ◽  
Feline Immunodeficiency Virus ◽  
Virus Vectors ◽  
Immunodeficiency Virus

Feline immunodeficiency virus vectors in gene therapy

2004 ◽  
Vol 9 (1) ◽  
Author(s):  
Flávia H. Da Silva ◽  
Camila M. Ilgenfritz ◽  
Nance B. Nardi
Keyword(s):  
Gene Therapy ◽  
Feline Immunodeficiency Virus ◽  
Virus Vectors ◽  
Immunodeficiency Virus

scholarly journals Expression from Second-Generation Feline Immunodeficiency Virus Vectors Is Impaired in Human Hematopoietic Cells

2002 ◽  
Vol 6 (5) ◽  
pp. 645-652
Author(s):  
Mary A. Price ◽  
Scott S. Case ◽  
Denise A. Carbonaro ◽  
Xiao-Jin Yu ◽  
Denise Petersen ◽  
...  
Keyword(s):  
Feline Immunodeficiency Virus ◽  
Second Generation ◽  
Hematopoietic Cells ◽  
Virus Vectors ◽  
Immunodeficiency Virus

scholarly journals 697. Molecular Forms of Feline Immunodeficiency Virus Following Gene Transfer to Airway Epithelia

2006 ◽  
Vol 13 ◽  
pp. S270
Author(s):  
Marisa Banasik ◽  
Christopher Moressi ◽  
Melissa Hickey ◽  
Todd Scheetz ◽  
Paul McCray
Keyword(s):  
Gene Transfer ◽  
Feline Immunodeficiency Virus ◽  
Molecular Forms ◽  
Airway Epithelia ◽  
Immunodeficiency Virus
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