scholarly journals Endothelial cell culture: beginnings of modern vascular biology

2004 ◽  
Vol 114 (8) ◽  
pp. 1037-1040 ◽  
Author(s):  
Ralph L. Nachman ◽  
Eric A. Jaffe
Keyword(s):  
Cell Culture ◽  
Endothelial Cell ◽  
Vascular Biology ◽  
Endothelial Cell Culture

Cerebrovascular Endothelial Cell Culture: Metabolism and Synthesis of 5-Hydroxytryptamine

1984 ◽  
Vol 43 (2) ◽  
pp. 316-319 ◽  
Author(s):  
C. Maruki ◽  
M. Spatz ◽  
Y. Ueki ◽  
I. Nagatsu ◽  
J. Bembry
Keyword(s):  
Cell Culture ◽  
Endothelial Cell ◽  
Endothelial Cell Culture

MO710GALECTIN-3, IS IT A NEW PLAYER IN PERITONEAL INFLAMMATION AMONG PATIENTS UNDERGOING PERITONEAL DIALYSIS?

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
Yael Einbinder ◽  
Keren Cohen-Hagai ◽  
Sydney Benchetrit ◽  
Tali Zitman-Gal
Keyword(s):  
Cell Culture ◽  
Peritoneal Dialysis ◽  
Endothelial Cell ◽  
Protein Expression ◽  
Inflammatory Process ◽  
Peritoneal Membrane ◽  
Peritoneal Equilibration Test ◽  
Endothelial Cell Culture ◽  
Dialysis Vintage ◽  
Mrna And Protein Expression

Abstract Background and Aims Peritoneal dialysis (PD) is a common used method for renal replacement therapy. Prolonged PD treatment causes structural and functional changes in the peritoneal membrane which are attributed to local inflammatory process in the peritoneal cavity. Galectin-3 (Gal-3) is a galactoside-binding lectin with pro-inflammatory and pro-fibrotic effects. The aim of this study was to assess correlation between Gal-3 serum and dialysate effluent levels with peritoneal membrane transport characteristics. Method Gal-3 levels in serum and dialysate effluent were measured simultaneously in prevalent PD patients in morning visit or during peritoneal equilibration test (PET). Gal-3 levels were correlated with clinical and laboratory parameters. Interlukin (IL) -6 levels were measured in dialysate effluent. Gal-3 mRNA and protein expression were evaluated after exposure of primary endothelial cell culture to several dialysate solutions. Results 37 PD patients were included in the study; mean age was 65.7±13.1 years, mean dialysis vintage was 17.5±13 months. Gal-3 levels in dialysate effluent correlated with peritoneal equilibration test (PET) results (0.663, p=0.005) and effluent IL-6 levels (0.674, p=0.002) but not with serum Gal-3 levels or dialysis vintage. Patients with high PET results had higher effluent Gal-3 levels as compared average low PET results. In multivariate regression analysis effluent IL-6 level was the most dominant predictor of effluent Gal-3 levels. Gal-3 mRNA and protein expression in primary endothelial cell culture were not affected by stimulation with dialysate solutions. Conclusion Our study demonstrated presence of Gal-3 within the dialysate effluent in PD patients. Gal-3 levels correlated with peritoneal membrane transport characteristics and effluent IL-6 levels suggesting a role in the inflammatory process within the peritoneal cavity.

scholarly journals Growth and Function of the Endothelial Cell Culture in the Pressure Chamber

1984 ◽  
Vol 12 (4) ◽  
pp. 771-773
Author(s):  
Osamu TOKUNAGA ◽  
Ryuji NAKANO ◽  
Toshihiko KINOSHITA ◽  
Minoru MORIMATSU ◽  
Teruyuki NAKASHIMA
Keyword(s):  
Cell Culture ◽  
Endothelial Cell ◽  
Pressure Chamber ◽  
Endothelial Cell Culture ◽  
And Function

From square to circular polymeric microchannels by spin coating technology: a low cost platform for endothelial cell culture

2016 ◽  
Vol 8 (2) ◽  
pp. 025005 ◽  
Author(s):  
Raffaele Vecchione ◽  
Gabriele Pitingolo ◽  
Daniela Guarnieri ◽  
Andrea P Falanga ◽  
Paolo A Netti
Keyword(s):  
Cell Culture ◽  
Endothelial Cell ◽  
Spin Coating ◽  
Low Cost ◽  
Coating Technology ◽  
Endothelial Cell Culture

scholarly journals Making cell culture more physiological: a call for a more comprehensive assessment of racial disparities in endothelial cell culture studies

2020 ◽  
Vol 318 (2) ◽  
pp. C238-C241 ◽  
Author(s):  
Austin T. Robinson ◽  
Marc D. Cook ◽  
Abbi D. Lane-Cordova
Keyword(s):  
Cell Culture ◽  
Endothelial Cell ◽  
Racial Disparities ◽  
Racial Differences ◽  
White Women ◽  
Microvascular Complications ◽  
The United States ◽  
Culture Studies ◽  
Endothelial Cell Function ◽  
Endothelial Cell Culture

In the United States, cardiovascular diseases (CVDs) are the leading cause of death and disproportionately affect ethnic and racial minority populations. Black individuals are more likely to develop advanced CVD and microvascular complications resulting in end-organ damage. Endothelial cell dysfunction leads to microvascular and macrovascular dysfunction and is predictive of the development of CVD. Black versus white racial disparities in in vivo and in vitro studies of endothelial cell function are well documented. However, race-related disparities in maternal environment and lifestyle may be a major unconsidered factor in racial differences in endothelial cell culture studies. Further, rates of hypertensive disorders of pregnancy are higher in black versus white women. These pregnancy complications may result in placental dysfunction, including excess production of inflammatory and antiangiogenic molecules that impair endothelial function. Therefore, studies that include other ethnic and racial minorities are needed, in addition to a more thorough characterization of endothelial cell donors and targeted cell culture studies (e.g., genotyping) to generate information that can be translated into effective preventive or treatment strategies for ethnic/racial disparities in CVD.

Effect of low-level laser and antivenom therapies of Philodryas olfersii (Lichtenstein, 1823) venom on endothelial cell culture

Toxicon ◽  
2020 ◽  
Vol 177 ◽  
pp. S48
Author(s):  
Jose Cogo ◽  
Marcio Vicente Ferreira ◽  
Mozart Leopoldino ◽  
Jessia O.S. Fernandes ◽  
Luciana M.G. Silva ◽  
...  
Keyword(s):  
Cell Culture ◽  
Endothelial Cell ◽  
Endothelial Cell Culture ◽  
Low Level ◽  
Low Level Laser ◽  
Level Laser

Temporal Profile of Matrix Metalloproteinases and Their Inhibitors in a Human Endothelial Cell Culture Model of Cerebral Ischemia

2013 ◽  
Vol 35 (6) ◽  
pp. 514-520 ◽  
Author(s):  
Björn Reuter ◽  
Claus Rodemer ◽  
Saskia Grudzenski ◽  
Pierre-Olivier Couraud ◽  
Babette Weksler ◽  
...  
Keyword(s):  
Cell Culture ◽  
Endothelial Cell ◽  
Cerebral Ischemia ◽  
Matrix Metalloproteinases ◽  
Human Endothelial Cell ◽  
Cell Culture Model ◽  
Endothelial Cell Culture ◽  
Temporal Profile ◽  
Culture Model
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