scholarly journals Intestinal heat shock protein 110 regulates expression of CD1d on intestinal epithelial cells

2003 ◽  
Vol 112 (5) ◽  
pp. 745-754 ◽  
Author(s):  
Sean P. Colgan ◽  
Richard S. Pitman ◽  
Takashi Nagaishi ◽  
Atsushi Mizoguchi ◽  
Emiko Mizoguchi ◽  
...  
Keyword(s):  
Heat Shock ◽  
Epithelial Cells ◽  
Heat Shock Protein ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial

Overexpression of a 60-kDa heat shock protein enhances cytoprotective function of small intestinal epithelial cells

Life Sciences ◽  
2010 ◽  
Vol 86 (13-14) ◽  
pp. 499-504 ◽  
Author(s):  
Makiko Takada ◽  
Michiro Otaka ◽  
Taiji Takahashi ◽  
Yuko Izumi ◽  
Kumiko Tamaki ◽  
...  
Keyword(s):  
Heat Shock ◽  
Epithelial Cells ◽  
Heat Shock Protein ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial ◽  
Small Intestinal

Intestinal lumen-derived heat shock protein 110 regulates expression of the MHC-class I-like molecule CD1d on intestinal epithelial cells

2003 ◽  
Vol 124 (4) ◽  
pp. A82-A83 ◽  
Author(s):  
Sean P. Colgan ◽  
Takashi Nagaishi ◽  
Atsushi Mizoguchi ◽  
Emiko Mizoguchi ◽  
Lloyd Mayer ◽  
...  
Keyword(s):  
Heat Shock ◽  
Epithelial Cells ◽  
Heat Shock Protein ◽  
Mhc Class I ◽  
Intestinal Epithelial Cells ◽  
Class I ◽  
Intestinal Lumen ◽  
Intestinal Epithelial

Heat induction of heat shock protein 25 requires cellular glutamine in intestinal epithelial cells

2006 ◽  
Vol 291 (2) ◽  
pp. C290-C299 ◽  
Author(s):  
Kittiporn Phanvijhitsiri ◽  
Mark W. Musch ◽  
Mark J. Ropeleski ◽  
Eugene B. Chang
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Heat Shock ◽  
Epithelial Cells ◽  
Heat Shock Protein ◽  
Cellular Response ◽  
Intestinal Epithelial Cells ◽  
Dependent Manner ◽  
Intestinal Epithelial ◽  
Heat Induction

Glutamine is considered a nonessential amino acid; however, it becomes conditionally essential during critical illness when consumption exceeds production. Glutamine may modulate the heat shock/stress response, an important adaptive cellular response for survival. Glutamine increases heat induction of heat shock protein (Hsp) 25 in both intestinal epithelial cells (IEC-18) and mesenchymal NIH/3T3 cells, an effect that is neither glucose nor serum dependent. Neither arginine, histidine, proline, leucine, asparagine, nor tyrosine acts as physiological substitutes for glutamine for heat induction of Hsp25. The lack of effect of these amino acids was not caused by deficient transport, although some amino acids, including glutamate (a major direct metabolite of glutamine), were transported poorly by IEC-18 cells. Glutamate uptake could be augmented in a concentration- and time-dependent manner by increasing either media concentration and/or duration of exposure. Under these conditions, glutamate promoted heat induction of Hsp25, albeit not as efficiently as glutamine. Further evidence for the role of glutamine conversion to glutamate was obtained with the glutaminase inhibitor 6-diazo-5-oxo-l-norleucine (DON), which inhibited the effect of glutamine on heat-induced Hsp25. DON inhibited phosphate-dependent glutaminase by 75% after 3 h, decreasing cell glutamate. Increased glutamine/glutamate conversion to glutathione was not involved, since the glutathione synthesis inhibitor, buthionine sulfoximine, did not block glutamine’s effect on heat induction of Hsp25. A large drop in ATP levels did not appear to account for the diminished Hsp25 induction during glutamine deficiency. In summary, glutamine is an important amino acid, and its requirement for heat-induced Hsp25 supports a role for glutamine supplementation to optimize cellular responses to pathophysiological stress.

scholarly journals Threonine treatment increases heat shock protein 25 and 70 expression and decreases apoptosis in heat stressed intestinal epithelial cells

2012 ◽  
Vol 26 (S1) ◽  
Author(s):  
Christine Hamiel Baird ◽  
Alyssa Rene Kallweit ◽  
Ryan Beck ◽  
Stefanie Niederlechner ◽  
Paul Edmund Wischmeyer
Keyword(s):  
Heat Shock ◽  
Epithelial Cells ◽  
Heat Shock Protein ◽  
Intestinal Epithelial Cells ◽  
Intestinal Epithelial
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