scholarly journals Cancer-associated mesothelial cells promote ovarian cancer chemoresistance through paracrine osteopontin signaling

2021 ◽  
Vol 131 (16) ◽  
Author(s):  
Jin Qian ◽  
Bauer L. LeSavage ◽  
Kelsea M. Hubka ◽  
Chenkai Ma ◽  
Suchitra Natarajan ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Mesothelial Cells ◽  
Cancer Chemoresistance

scholarly journals LncRNA SPOCD1-AS from ovarian cancer extracellular vesicles remodels mesothelial cells to promote peritoneal metastasis via interacting with G3BP1

2021 ◽  
Vol 40 (1) ◽  
Author(s):  
Conghui Wang ◽  
Jiaying Wang ◽  
Xiameng Shen ◽  
Mingyue Li ◽  
Yongfang Yue ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Extracellular Vesicles ◽  
Peritoneal Metastasis ◽  
Cancer Metastasis ◽  
Cancer Therapeutics ◽  
Mesothelial Cells ◽  
Ovarian Cancer Cells ◽  
Adhesion Assay ◽  
Mesenchymal Transition

Abstract Background Metastasis is the key cause of death in ovarian cancer patients. To figure out the biological nature of cancer metastasis is essential for developing effective targeted therapy. Here we investigate how long non-coding RNA (lncRNA) SPOCD1-AS from ovarian cancer extracellular vesicles (EVs) remodel mesothelial cells through a mesothelial-to-mesenchymal transition (MMT) manner and facilitate peritoneal metastasis. Methods EVs purified from ovarian cancer cells and ascites of patients were applied to mesothelial cells. The MMT process of mesothelial cells was assessed by morphology observation, western blot analysis, migration assay and adhesion assay. Altered lncRNAs of EV-treated mesothelial cells were screened by RNA sequencing and identified by qRT-PCR. SPOCD1-AS was overexpressed or silenced by overexpression lentivirus or shRNA, respectively. RNA pull-down and RNA immunoprecipitation assays were conducted to reveal the mechanism by which SPOCD1-AS remodeled mesothelial cells. Interfering peptides were synthesized and applied. Ovarian cancer orthotopic implantation mouse model was established in vivo. Results We found that ovarian cancer-secreted EVs could be taken into recipient mesothelial cells, induce the MMT phenotype and enhance cancer cell adhesion to mesothelial cells. Furthermore, SPOCD1-AS embedded in ovarian cancer-secreted EVs was transmitted to mesothelial cells to induce the MMT process and facilitate peritoneal colonization in vitro and in vivo. SPOCD1-AS induced the MMT process of mesothelial cells via interacting with G3BP1 protein. Additionally, G3BP1 interfering peptide based on the F380/F382 residues was able to block SPOCD1-AS/G3BP1 interaction, inhibit the MMT phenotype of mesothelial cells, and diminish peritoneal metastasis in vivo. Conclusions Our findings elucidate the mechanism associated with EVs and their cargos in ovarian cancer peritoneal metastasis and may provide a potential approach for metastatic ovarian cancer therapeutics.

scholarly journals Senescent Peritoneal Mesothelial Cells Promote Ovarian Cancer Cell Adhesion

2009 ◽  
Vol 174 (4) ◽  
pp. 1230-1240 ◽  
Author(s):  
Krzysztof Ksiazek ◽  
Justyna Mikula-Pietrasik ◽  
Katarzyna Korybalska ◽  
Grzegorz Dworacki ◽  
Achim Jörres ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Adhesion ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Mesothelial Cells ◽  
Peritoneal Mesothelial Cells ◽  
Cancer Cell Adhesion

scholarly journals The exosome-mediated autocrine and paracrine actions of plasma gelsolin in ovarian cancer chemoresistance

Oncogene ◽  
2019 ◽  
Vol 39 (7) ◽  
pp. 1600-1616 ◽  
Author(s):  
Meshach Asare-Werehene ◽  
Kiran Nakka ◽  
Arkadiy Reunov ◽  
Chen-Tzu Chiu ◽  
Wei-Ting Lee ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cell Fate ◽  
Gynecological Cancer ◽  
Late Presentation ◽  
High Recurrence Rate ◽  
Free Survival ◽  
Plasma Gelsolin ◽  
Elevated Expression ◽  
Paracrine Actions ◽  
Cancer Chemoresistance

Abstract Ovarian cancer (OVCA) is the most lethal gynecological cancer, due predominantly to late presentation, high recurrence rate and common chemoresistance development. The expression of the actin-associated protein cytosolic gelsolin (GSN) regulates the gynecological cancer cell fate resulting in dysregulation in chemosensitivity. In this study, we report that elevated expression of plasma gelsolin (pGSN), a secreted isoform of GSN and expressed from the same GSN gene, correlates with poorer overall survival and relapse-free survival in patients with OVCA. In addition, it is highly expressed and secreted in chemoresistant OVCA cells than its chemosensitive counterparts. pGSN, secreted and transported via exosomes (Ex-pGSN), upregulates HIF1α–mediated pGSN expression in chemoresistant OVCA cells in an autocrine manner as well as confers cisplatin resistance in otherwise chemosensitive OVCA cells. These findings support our hypothesis that exosomal pGSN promotes OVCA cell survival through both autocrine and paracrine mechanisms that transform chemosensitive cells to resistant counterparts. Specifically, pGSN transported via exosomes is a determinant of chemoresistance in OVCA.

scholarly journals A novel mechanism of neovascularization in peritoneal dissemination via cancer-associated mesothelial cells affected by TGF-β derived from ovarian cancer

2017 ◽  
Author(s):  
Kayo Fujikake ◽  
Hiroaki Kajiyama ◽  
Masato Yoshihara ◽  
Kimihiro Nishino ◽  
Nobuhisa Yoshikawa ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Peritoneal Dissemination ◽  
Mesothelial Cells

Mitochondria-related oxidative stress contributes to ovarian cancer-promoting activity of mesothelial cells subjected to malignant ascites

2018 ◽  
Vol 98 ◽  
pp. 82-88 ◽  
Author(s):  
Martyna Pakuła ◽  
Justyna Mikuła-Pietrasik ◽  
Łukasz Stryczyński ◽  
Paweł Uruski ◽  
Sebastian Szubert ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Ovarian Cancer ◽  
Malignant Ascites ◽  
Mesothelial Cells

scholarly journals Use of L-plastin promoter to develop an adenoviral system that confers transgene expression in ovarian cancer cells but not in normal mesothelial cells

1999 ◽  
Vol 6 (2) ◽  
pp. 99-106 ◽  
Author(s):  
Injae Chung ◽  
Peter E Schwartz ◽  
Ronald G Crystal ◽  
Giuseppe Pizzorno ◽  
John Leavitt ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Transgene Expression ◽  
Mesothelial Cells ◽  
Ovarian Cancer Cells

scholarly journals Inhibition of HDAC1 and DNMT1 Modulate RGS10 Expression and Decrease Ovarian Cancer Chemoresistance

PLoS ONE ◽  
2014 ◽  
Vol 9 (1) ◽  
pp. e87455 ◽  
Author(s):  
Ercan Cacan ◽  
Mourad W. Ali ◽  
Nathaniel H. Boyd ◽  
Shelley B. Hooks ◽  
Susanna F. Greer
Keyword(s):  
Ovarian Cancer ◽  
Cancer Chemoresistance
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