scholarly journals Disrupted ER membrane protein complex–mediated topogenesis drives congenital neural crest defects

2020 ◽  
Vol 130 (2) ◽  
pp. 813-826 ◽  
Author(s):  
Jonathan Marquez ◽  
June Criscione ◽  
Rebekah M. Charney ◽  
Maneeshi S. Prasad ◽  
Woong Y. Hwang ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Neural Crest ◽  
Protein Complex ◽  
Membrane Protein Complex ◽  
Er Membrane

scholarly journals The ER membrane protein complex promotes biogenesis of sterol-related enzymes maintaining cholesterol homeostasis

2018 ◽  
Vol 132 (2) ◽  
pp. jcs223453 ◽  
Author(s):  
Norbert Volkmar ◽  
Maria-Laetitia Thezenas ◽  
Sharon M. Louie ◽  
Szymon Juszkiewicz ◽  
Daniel K. Nomura ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Complex ◽  
Cholesterol Homeostasis ◽  
Membrane Protein Complex ◽  
Er Membrane

scholarly journals The ER Membrane Protein Complex Promotes Biogenesis of Dengue and Zika Virus Non-structural Multi-pass Transmembrane Proteins to Support Infection

Cell Reports ◽  
2019 ◽  
Vol 27 (6) ◽  
pp. 1666-1674.e4 ◽  
Author(s):  
David L. Lin ◽  
Takamasa Inoue ◽  
Yu-Jie Chen ◽  
Aaron Chang ◽  
Billy Tsai ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Zika Virus ◽  
Protein Complex ◽  
Transmembrane Proteins ◽  
Membrane Protein Complex ◽  
Er Membrane

scholarly journals The ubiquitous and ancient ER membrane protein complex (EMC): tether or not?

F1000Research ◽  
2015 ◽  
Vol 4 ◽  
pp. 624 ◽  
Author(s):  
Jeremy G. Wideman
Keyword(s):  
Membrane Protein ◽  
Protein Complex ◽  
Common Ancestor ◽  
Eukaryotic Cells ◽  
Eukaryotic Lineage ◽  
Membrane Protein Complex ◽  
Outer Membranes ◽  
Homology Searching ◽  
Eukaryote Evolution ◽  
Er Membrane

The recently discovered endoplasmic reticulum (ER) membrane protein complex (EMC) has been implicated in ER-associated degradation (ERAD), lipid transport and tethering between the ER and mitochondrial outer membranes, and assembly of multipass ER-membrane proteins. The EMC has been studied in both animals and fungi but its presence outside the Opisthokont clade (animals + fungi + related protists) has not been demonstrated. Here, using homology-searching algorithms, I show that the EMC is truly an ancient and conserved protein complex, present in every major eukaryotic lineage. Very few organisms have completely lost the EMC, and most, even over 2 billion years of eukaryote evolution, have retained a majority of the complex members. I identify Sop4 and YDR056C in Saccharomyces cerevisiae as Emc7 and Emc10, respectively, subunits previously thought to be specific to animals. This study demonstrates that the EMC was present in the last eukaryote common ancestor (LECA) and is an extremely important component of eukaryotic cells even though its primary function remains elusive.

scholarly journals The ubiquitous and ancient ER membrane protein complex (EMC): tether or not?

F1000Research ◽  
2015 ◽  
Vol 4 ◽  
pp. 624 ◽  
Author(s):  
Jeremy G. Wideman
Keyword(s):  
Membrane Protein ◽  
Protein Complex ◽  
Common Ancestor ◽  
Eukaryotic Cells ◽  
Eukaryotic Lineage ◽  
Membrane Protein Complex ◽  
Outer Membranes ◽  
Homology Searching ◽  
Eukaryote Evolution ◽  
Er Membrane

The recently discovered endoplasmic reticulum (ER) membrane protein complex (EMC) has been implicated in ER-associated degradation (ERAD), lipid transport and tethering between the ER and mitochondrial outer membranes, and assembly of multipass ER-membrane proteins. The EMC has been studied in both animals and fungi but its presence outside the Opisthokont clade (animals + fungi + related protists) has not been demonstrated. Here, using homology-searching algorithms, I show that the EMC is truly an ancient and conserved protein complex, present in every major eukaryotic lineage. Very few organisms have completely lost the EMC, and most, even over 2 billion years of eukaryote evolution, have retained a majority of the complex members. I identify Sop4 and YDR056C in Saccharomyces cerevisiae as Emc7 and Emc10, respectively, subunits previously thought to be specific to animals. This study demonstrates that the EMC was present in the last eukaryote common ancestor (LECA) and is an extremely important component of eukaryotic cells even though its primary function remains elusive.

scholarly journals Loss of the ER membrane protein complex subunit Emc3 leads to retinal bipolar cell degeneration in aged mice

PLoS ONE ◽  
2020 ◽  
Vol 15 (9) ◽  
pp. e0238435
Author(s):  
Xiong Zhu ◽  
Xin Qi ◽  
Yeming Yang ◽  
Wanli Tian ◽  
Wenjing Liu ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Complex ◽  
Bipolar Cell ◽  
Cell Degeneration ◽  
Membrane Protein Complex ◽  
Aged Mice ◽  
Er Membrane

scholarly journals Structural basis for membrane insertion by the human ER membrane protein complex

Science ◽  
2020 ◽  
pp. eabb5008 ◽  
Author(s):  
Tino Pleiner ◽  
Giovani Pinton Tomaleri ◽  
Kurt Januszyk ◽  
Alison J. Inglis ◽  
Masami Hazu ◽  
...  
Keyword(s):  
Membrane Protein ◽  
Protein Complex ◽  
Structural Basis ◽  
Membrane Insertion ◽  
Transmembrane Helices ◽  
Membrane Protein Complex ◽  
Cryo Electron Microscopy ◽  
Membrane Thinning ◽  
Positively Charged ◽  
Er Membrane

A defining step in the biogenesis of a membrane protein is the insertion of its hydrophobic transmembrane helices into the lipid bilayer. The nine-subunit ER membrane protein complex (EMC) is a conserved co- and post-translational insertase at the endoplasmic reticulum. We determined the structure of the human EMC in a lipid nanodisc to an overall resolution of 3.4 Å by cryo-electron microscopy, permitting building of a nearly complete atomic model. We used structure-guided mutagenesis to demonstrate that substrate insertion requires a methionine-rich cytosolic loop and occurs via an enclosed hydrophilic vestibule within the membrane formed by the subunits EMC3 and EMC6. We propose that the EMC uses local membrane thinning and a positively charged patch to decrease the energetic barrier for insertion into the bilayer.

scholarly journals ER membrane protein complex subunit 6 (EMC6) is a novel tumor suppressor in gastric cancer

BMB Reports ◽  
2017 ◽  
Vol 50 (8) ◽  
pp. 411-416 ◽  
Author(s):  
Xiaokun Wang ◽  
Yan Xia ◽  
Chentong Xu ◽  
Xin Lin ◽  
Peng Xue ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Tumor Suppressor ◽  
Membrane Protein ◽  
Protein Complex ◽  
Membrane Protein Complex ◽  
Er Membrane
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