scholarly journals Injurious ventilatory strategies increase cytokines and c-fos m-RNA expression in an isolated rat lung model.

1997 ◽  
Vol 99 (5) ◽  
pp. 944-952 ◽  
Author(s):  
L Tremblay ◽  
F Valenza ◽  
S P Ribeiro ◽  
J Li ◽  
A S Slutsky
Keyword(s):  
Lung Model ◽  
Rna Expression ◽  
Rat Lung ◽  
Isolated Rat Lung ◽  
Isolated Rat

Reduction of oedema formation after preconditioning with dopamine in an isolated rat lung model is mediated by adrenergic receptors

2011 ◽  
Vol 16 (3) ◽  
pp. 97-107 ◽  
Author(s):  
Christine Hanusch ◽  
Charlotte Hauser ◽  
Antje Geisler ◽  
Kai Nowak ◽  
Andreas Dacho ◽  
...  
Keyword(s):  
Adrenergic Receptors ◽  
Lung Model ◽  
Rat Lung ◽  
Oedema Formation ◽  
Isolated Rat Lung ◽  
Isolated Rat

Muscarinic agonists and antagonists cause vasodilation in isolated rat lung

1995 ◽  
Vol 78 (4) ◽  
pp. 1404-1411 ◽  
Author(s):  
P. S. Wilson ◽  
P. L. Khimenko ◽  
J. W. Barnard ◽  
T. M. Moore ◽  
A. E. Taylor
Keyword(s):  
Airway Pressure ◽  
Lung Model ◽  
Peak Airway Pressure ◽  
Receptor Subtypes ◽  
Rat Lung ◽  
Muscarinic Agonists ◽  
Selective Antagonist ◽  
M1 Receptors ◽  
Isolated Rat Lung ◽  
Isolated Rat

The present study investigated the ability of atropine and different muscarinic receptor subtypes to affect acetylcholine (ACh)-induced bronchoconstriction and vasodilation in the isolated rat lung model. ACh (10(-7) M) given after U-46619 decreased total (RT), precapillary, and postcapillary vascular resistances and increased peak airway pressure. Atropine (20 microM) decreased RT and precapillary and postcapillary vascular resistances and blocked ACh-induced increases in peak airway pressure. The M1-selective agonist McN-A-343 (1.3 x 10(-5) M) decreased RT from 40.27 +/- 2.98 to 29.20 +/- 2.81 cmH2O.l–1.min-100 g lung wt (P = 0.01), and ACh caused no further dilation. The M1-selective antagonist pirenzepine (1.6 x 10(-6) M) blocked ACh-induced vasodilation. The M2-selective antagonist gallamine (7.5 x 10(-7) M) decreased RT from 45.50 +/- 3.19 to 34.86 +/- 1.25 cmH2O.l–1.min.100 g lung wt (P < 0.05), and after gallamine, ACh further decreased RT to 28.59 +/- 1.75 cmH2O.l–1.min.100 g lung wt (P < 0.01). Neither the selective muscarinic agonists nor antagonists affected peak airway pressures. We conclude that ACh-induced vasodilation in isolated rat lungs preconstricted with U-46619 is mediated by M1 receptors. Atropine-induced vasodilation in this model is mediated through the inhibition of the M2 receptor. We postulate that this represents either a blockade of postganglionic receptors, permitting release of vasodilator substances from local nerve terminals, or a direct vasodilatory effect on the vascular smooth muscle.

PGE1, dexamethasone, U-74389G, or Bt2-cAMP as an additive to promote protection by UW solution in I/R injury

1997 ◽  
Vol 83 (2) ◽  
pp. 583-590 ◽  
Author(s):  
Chi-Huei Chiang ◽  
Kang Hsu ◽  
Horng-Chin Yan ◽  
Horng-Jyh Harn ◽  
Deh-Ming Chang
Keyword(s):  
Protein Concentration ◽  
Lavage Fluid ◽  
Lung Model ◽  
Rat Lung ◽  
Preservation Solution ◽  
Uw Solution ◽  
Protective Agents ◽  
Tnf Α ◽  
Isolated Rat Lung ◽  
Isolated Rat

Chiang, Chi-Huei, Kang Hsu, Horng-Chin Yan, Horng-Jyh Harn, and Deh-Ming Chang.PGE1, dexamethasone, U-74389G, or Bt2-cAMP as an additive to promote protection by UW solution in I/R injury. J. Appl. Physiol. 83(2): 583–590, 1997.—A method to reduce ischemia-reperfusion (I/R) injury can be an important criterion to improve the preservation solution. Although University of Wisconsin solution (UW) works as a lung preservation solution, its attenuation effect on I/R injury has not been investigated. We attempted to determine whether, by adding various protective agents, modified UW solutions will enhance the I/R attenuation by UW. We examined the I/R injury in an isolated rat lung model. Various solutions, e.g., physiological salt solution (PSS), UW, and modified UW solutions containing various protective agents such as prostaglandin E1, dexamethasone, U-74389G, or dibutyryl adenosine 3′,5′-cyclic monophosphate were perfused individually to evaluate the I/R injury. Isolated rat lung experiments, with ischemia for 45 min, then reperfusion for 60 min, were conducted in a closed circulating system. Hemodynamic changes, lung weight gain (LWG), capillary filtration coefficient ( Kfc), protein content of lavage fluid, concentration of cytokines, and lung histopathology were analyzed. Results showed that the acute I/R lung injury with immediate permeability pulmonary edema was associated with an increase in tumor necrosis factor-α (TNF-α) production. A significant correlation existed between TNF-α and Kfc( r = 0.8, P < 0.0001) and TNF-α and LWG ( r = 0.9, P < 0.0001), indicating that TNF-α is an important cytokine modulating early I/R injury. Significantly lower levels of Kfc, LWG, TNF-α, and protein concentration of lung lavage ( P < 0.05) were found in the UW-perfused group than in the control group perfused with PSS. Modified UW promoted the protective effect of UW to further decrease Kfc, LWG, and TNF-α ( P < 0.05). Histopathological observations also substantiated this evidence. In the UW+U-74389G group, bronchial alveolar lavage fluid contained lowest protein concentration. We conclude that the UW solution attenuates I/R injury of rat lung and that the modified UW solutions further enhance the effect of UW in reducing I/R injury. Among modified solutions, UW+U-74389G is the best. Further investigation of the improved effects of the modified UW solutions would be beneficial in lung transplantation.

Cytosolic Phospholipase A2 Inhibition Attenuates Ischemia-Reperfusion Injury in an Isolated Rat Lung Model

2006 ◽  
Vol 81 (12) ◽  
pp. 1700-1707 ◽  
Author(s):  
Yury A. Bellido-Reyes ◽  
Hideki Akamatsu ◽  
Katsuo Kojima ◽  
Hirokuni Arai ◽  
Hiroyuki Tanaka ◽  
...  
Keyword(s):  
Phospholipase A2 ◽  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Cytosolic Phospholipase A2 ◽  
Lung Model ◽  
Rat Lung ◽  
Cytosolic Phospholipase ◽  
Isolated Rat Lung ◽  
Isolated Rat

scholarly journals Aprotinin attenuated ischemia–reperfusion injury in an isolated rat lung model after 18-hours preservation

2005 ◽  
Vol 28 (4) ◽  
pp. 581-587 ◽  
Author(s):  
Takehiko Shimoyama ◽  
Noriyuki Tabuchi ◽  
Katsuo Kojima ◽  
Hideki Akamatsu ◽  
Hirokuni Arai ◽  
...  
Keyword(s):  
Reperfusion Injury ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Lung Model ◽  
Rat Lung ◽  
Isolated Rat Lung ◽  
Isolated Rat

Establishment and validation of an isolated rat lung model for pulmonary metabolism studies

1999 ◽  
Vol 19 (2) ◽  
pp. 83-91 ◽  
Author(s):  
David G. Baker ◽  
Bruce R. Toth ◽  
Mary E. P. Goad ◽  
Steven A. Barker ◽  
Jay C. Means
Keyword(s):  
Lung Model ◽  
Rat Lung ◽  
Isolated Rat Lung ◽  
Isolated Rat

Pulmonary preservation effect of nitroglycerine in isolated rat lung reperfusion model

2004 ◽  
Vol 36 (7) ◽  
pp. 1933-1935 ◽  
Author(s):  
S. Jheon ◽  
Y.M. Lee ◽  
S.W. Sung ◽  
J.H. Choh ◽  
K.Y. Kwon ◽  
...  
Keyword(s):  
Rat Lung ◽  
Isolated Rat Lung ◽  
Isolated Rat

High airway-to-blood transport of an opioid tetrapeptide in the isolated rat lung after aerosol delivery

Peptides ◽  
2002 ◽  
Vol 23 (3) ◽  
pp. 469-478 ◽  
Author(s):  
Ann Tronde ◽  
Eva Krondahl ◽  
Hans von Euler-Chelpin ◽  
Per Brunmark ◽  
Ursula Hultkvist Bengtsson ◽  
...  
Keyword(s):  
Rat Lung ◽  
Aerosol Delivery ◽  
Blood Transport ◽  
Isolated Rat Lung ◽  
Isolated Rat
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