scholarly journals Shear stress induction of the tissue factor gene.

1997 ◽  
Vol 99 (4) ◽  
pp. 737-744 ◽  
Author(s):  
M C Lin ◽  
F Almus-Jacobs ◽  
H H Chen ◽  
G C Parry ◽  
N Mackman ◽  
...  
Keyword(s):  
Shear Stress ◽  
Tissue Factor ◽  
Stress Induction ◽  
Factor Gene

scholarly journals Fluid Shear Stress Induction of the Tissue Factor Promoter In Vitro and In Vivo Is Mediated by Egr-1

1999 ◽  
Vol 19 (2) ◽  
pp. 281-289 ◽  
Author(s):  
Parul Houston ◽  
Marion C. Dickson ◽  
Valerie Ludbrook ◽  
Brian White ◽  
Jean-Luc Schwachtgen ◽  
...  
Keyword(s):  
Shear Stress ◽  
Tissue Factor ◽  
Fluid Shear Stress ◽  
Fluid Shear ◽  
Stress Induction

scholarly journals Transcriptional Regulation of the Tissue Factor Gene in Human Epithelial Cells Is Mediated by Sp1 and EGR-1

1996 ◽  
Vol 271 (5) ◽  
pp. 2731-2739 ◽  
Author(s):  
Mei-Zhen Cui ◽  
Graham C.N. Parry ◽  
Paul Oeth ◽  
Heike Larson ◽  
Melissa Smith ◽  
...  
Keyword(s):  
Transcriptional Regulation ◽  
Epithelial Cells ◽  
Tissue Factor ◽  
Factor Gene ◽  
Human Epithelial Cells

Regulation of the tissue factor gene

1995 ◽  
Vol 9 (10) ◽  
pp. 883-889 ◽  
Author(s):  
Nigel Mackman
Keyword(s):  
Tissue Factor ◽  
Factor Gene

Role of Cbfa1/Runx2 in the fluid shear stress induction of COX-2 in osteoblasts

2006 ◽  
Vol 341 (4) ◽  
pp. 1225-1230 ◽  
Author(s):  
Meenal Mehrotra ◽  
Masatomo Saegusa ◽  
Olga Voznesensky ◽  
Carol Pilbeam
Keyword(s):  
Shear Stress ◽  
Fluid Shear Stress ◽  
Fluid Shear ◽  
Stress Induction ◽  
Cox 2

TISSUE FACTOR GENE TAG-SNPS AND HAPLOTYPES AND THE RISK OF MYOCARDIAL INFARCTION

2007 ◽  
Vol 5 ◽  
pp. P-M-003-P-M-003
Author(s):  
G. Quaquaruccio ◽  
M. Latella ◽  
M. Graziano ◽  
A. Di Castelnuovo ◽  
R. Lorenzet ◽  
...  
Keyword(s):  
Myocardial Infarction ◽  
Tissue Factor ◽  
Factor Gene ◽  
Tag Snps

scholarly journals The functional expression of tissue factor by fibroblasts and endothelial cells under flow conditions

Blood ◽  
1993 ◽  
Vol 81 (12) ◽  
pp. 3265-3270 ◽  
Author(s):  
EF Grabowski ◽  
DB Zuckerman ◽  
Y Nemerson
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Tissue Factor ◽  
Factor Viia ◽  
Factor Xa ◽  
Cell Types ◽  
Shear Stresses ◽  
Factor X ◽  
Flow Conditions ◽  
Production Values

Abstract The expression of tissue factor (TF) by a variety of vascular cell types under physiologic flow conditions is critical to factor X activation and in vivo clotting. Therefore, in a parallel-plate flow chamber (volume 40 microL) we mounted monolayers of human embryonic fibroblasts (FBs) or interleukin-1 alpha (IL-1 alpha) (5 U/mL x 4 hours)-stimulated human umbilical vein endothelial cells (ECs). Inflow buffer contained 10 nmol/L factor VIIa, 100 nmol/L factor X, and 2.0 mmol/L CaCl. With FBs, production of factor Xa (product of outflow concentration of factor Xa-and flow rate) increased 200-fold over the range of shear stress from 0 to 2.7 dynes/cm2. Production values (mean +/- SE (N)) were 7.93 +/- 0.024 (6), 312 +/- 7.3 (6), 688 +/- 33.1 (8), 1,033 +/- 119 (6), and 1,601 +/- 183 (7) fmol/cm2.minute at shear stresses of 0, 0.27, 0.68, 1.35, and 2.7 dynes/cm2, respectively. Further experiments at 0.68 dynes/cm2 indicated that factor Xa production increased with factor X concentration over the range from 3 to 100 nmol/L, but changed little from 300 to 1,000 nmol/L. With ECs, production was 0.13 +/- 0.86 (6), 8.17 +/- 1.65 (13), and 1.66 +/- 1.66 (5) fmol/cm2.minute at 0, 0.68, and 2.7 dynes/cm2, respectively. However, in the presence of an antibody directed against tissue factor pathway inhibitor (TFPI) production with ECs was augmented to 16.46 +/- 0.80 (8), 149.8 +/- 18.6 (8), and 48.9 +/- 10.3 (10), respectively, at these same shear stresses. Control experiments with factor VIIa, factor X, or both absent confirm for both cell types the specificity of the reaction for the TF pathway. Similarly, specificity for TF itself is shown by the virtual absence of factor Xa generation in the presence of the monoclonal antibody HTF1–7B8 directed against human TF. We conclude that ECs, even when activated, are normally unable to generate significant quantities of factor Xa in the presence of factors X and VIIa. However, significant quantities of factor Xa are possible in the presence of an inhibitor of TFPI. On the other hand, production of factor Xa by fibroblasts is markedly augmented by shear stress, yet independent of the availability of substrate factor X above an inflow concentration of 100 nmol/L. The latter suggests a direct effect of flow on the fibroblast monolayers, not substrate limitation by convective diffusion.

scholarly journals Tissue Factor Gene Expression Analysis in Circulating Monocytes*

2006 ◽  
Vol 33 (2) ◽  
pp. 183-188
Author(s):  
Jens Müller ◽  
Jutta Maria Rox ◽  
Bernd Pötzsch
Keyword(s):  
Gene Expression ◽  
Tissue Factor ◽  
Expression Analysis ◽  
Gene Expression Analysis ◽  
Factor Gene
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