scholarly journals Somatic mutation and CDR3 lengths of immunoglobulin kappa light chains expressed in patients with rheumatoid arthritis and in normal individuals.

1995 ◽  
Vol 96 (2) ◽  
pp. 831-841 ◽  
Author(s):  
S L Bridges ◽  
S K Lee ◽  
M L Johnson ◽  
J C Lavelle ◽  
P G Fowler ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Somatic Mutation ◽  
Light Chains ◽  
Immunoglobulin Kappa ◽  
Normal Individuals ◽  
Kappa Light Chains

scholarly journals Characterization of a unique conformational epitope on free immunoglobulin kappa light chains that is recognized by an antibody with therapeutic potential

2011 ◽  
Vol 48 (9-10) ◽  
pp. 1245-1252 ◽  
Author(s):  
Andrew T. Hutchinson ◽  
Ralitza Alexova ◽  
Vanessa Bockhorni ◽  
Paul A. Ramsland ◽  
Darren R. Jones ◽  
...  
Keyword(s):  
Therapeutic Potential ◽  
Conformational Epitope ◽  
Light Chains ◽  
Immunoglobulin Kappa ◽  
Kappa Light Chains

scholarly journals Somatic mutation creates diversity in the major group of mouse immunoglobulin kappa light chains.

1984 ◽  
Vol 159 (2) ◽  
pp. 417-435 ◽  
Author(s):  
G Heinrich ◽  
A Traunecker ◽  
S Tonegawa
Keyword(s):  
Somatic Mutations ◽  
Germ Line ◽  
Amino Acid Sequences ◽  
Light Chains ◽  
Chromosomal Dna ◽  
Immunoglobulin Kappa ◽  
Hypervariable Regions ◽  
Mouse Immunoglobulin ◽  
Kappa Light Chains ◽  
Cloned Cdna

Using a cloned cDNA of a mouse immunoglobulin kappa light chain synthesized in a myeloma MOPC321 (V kappa-21 subgroup C) as a probe we could detect 13 germ line V kappa gene segments. 11 of these were isolated. Using a set of overlapping cloned segments, we showed that nine of these germ line V kappa genes are arranged in two linkage clusters and that they all have the same transcriptional orientation (11, 12, 22). These two clusters occupy 90 and 30 kb of chromosomal DNA and contain six and three V kappa's, respectively. We determined the complete nucleotide sequences of five germ line V kappa's and showed that three of them encode the prototype sequence of V kappa-21 subgroups B, C, and E. None of these five germ line V kappa's encodes the variant amino acid sequences of known V kappa-21 subgroups. We thus conclude that, as in the lambda 1 light chains, the variant V regions are encoded by gene segments derived by a few somatic mutations from the corresponding germ line DNA. Such somatic mutations are not restricted to sequences encoding the hypervariable regions: they also occur in sequences encoding framework regions.

scholarly journals Sequence of the full-length immunoglobulin κ-chain of mouse myeloma MPC 11

1978 ◽  
Vol 171 (2) ◽  
pp. 337-347 ◽  
Author(s):  
G P Smith
Keyword(s):  
Variable Region ◽  
Full Length ◽  
Light Chains ◽  
Its Sequence ◽  
Heavy Chains ◽  
Immunoglobulin Kappa ◽  
N Terminus ◽  
Kappa Chain ◽  
Kappa Light Chains ◽  
Mouse Myeloma

MPC 11 mouse myeloma cells synthesize two immunoglobulin kappa light chains, coded by two separate genes. One of these Kappa-chains has no variable region and is degraded intracellularly. The other is a full-length kappa-chain contaning both variable and constant regions: this chain is secreted, both by itself and combined with heavy chains in molecules of immunoglobulin G. This paper reports the amino acid sequence of the myeloma MPC 11 full-length kappa-chain. The chain is unusual in having 12 extra residues at its N-terminus when its sequence is aligned with those of other mouse kappa-chains; no other anomalies were found in its sequence.

scholarly journals Sequence of a cDNA encoding Basilea kappa light chains (K2 isotype) suggests a possible relationship of protein structure to limited expression.

1984 ◽  
Vol 159 (2) ◽  
pp. 635-640 ◽  
Author(s):  
K E Bernstein ◽  
E Lamoyi ◽  
N McCartney-Francis ◽  
R G Mage
Keyword(s):  
Sulfhydryl Group ◽  
Variable Region ◽  
Complete Sequence ◽  
Light Chains ◽  
Small Subset ◽  
Constant Region ◽  
Region Gene ◽  
Variable Regions ◽  
Immunoglobulin Kappa ◽  
Kappa Light Chains

We present the complete sequence of a cDNA encoding rabbit immunoglobulin kappa light chains of the Basilea isotype (K2). Although all rabbits seem to possess a K2 constant region gene, expression of this gene in most rabbits is minimal if present at all. Even in Basilea rabbits the majority of expressed immunoglobulins are of lambda type. We find that the sequence of our Basilea cDNA constant region and the sequence of a "silent" K2 gene from b4 rabbits (bas-N4) are almost identical. The bas (K2) isotype lacks cysteine at position 171 in the constant region that is present in all K1 constant regions and usually forms an interdomain disulfide bond, with a cysteine at position 80 of the variable region. We postulate that one factor contributing to the low expression of the bas (K2) isotype could be a paucity of V kappa regions lacking cysteine at position 80. If a typical rabbit V kappa encoding Cys at position 80 is rearranged and expressed with th K2 isotype. B cells with mRNAs encoding light chains with free sulfhydryl groups would result. These cells may fail to form functional immunoglobulin receptors. Only a small subset of rabbit variable regions that lack the cysteine at position 80 would rearrange and encode K2 light chains lacking a free sulfhydryl group.

Sign in / Sign up

Export Citation Format

Share Document