scholarly journals Increased angiotensin-I converting enzyme gene expression in the failing human heart. Quantification by competitive RNA polymerase chain reaction.

1994 ◽  
Vol 94 (1) ◽  
pp. 301-310 ◽  
Author(s):  
R Studer ◽  
H Reinecke ◽  
B Müller ◽  
J Holtz ◽  
H Just ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Rna Polymerase ◽  
Human Heart ◽  
Converting Enzyme ◽  
Angiotensin I ◽  
Chain Reaction ◽  
Enzyme Gene ◽  
Angiotensin I Converting Enzyme ◽  
Polymerase Chain

Ace Gene Polymorphism Insertion Deletion Association with Prostate Cancer

2018 ◽  
Vol 9 (2) ◽  
Author(s):  
Hasanain Ali Shubbar ◽  
Mahfooda Abbas Umran
Keyword(s):  
Prostate Cancer ◽  
Gene Polymorphism ◽  
P Value ◽  
Converting Enzyme ◽  
Angiotensin I ◽  
Chain Reaction ◽  
Ace Gene ◽  
Ace Gene Polymorphism ◽  
Angiotensin I Converting Enzyme ◽  
Polymerase Chain

The angiotensin-converting enzyme (ACE) gene carries two alleles: insertion (I) and deletion (D) polymorphism inside its intron 16 . The study investigation the association between genetic polymorphisms and prostate patients. Materials and Methods: 75 prostate cancer patients, 75 prostate benign and 81 healthy were included. The ACE I/D genotypes were determined by PCR (polymerase chain reaction) Results showed for ACE gene polymorphism at that DD allele relation with prostate cancer p-value 0.0001** and prostate benign relation with ID allele p-value 0.0097**. This study aimed to detecting genetic early marker in angiotensin I converting enzyme (ACE) gene Iraqi patients with prostate carcinoma.

Relationships between angiotensin I converting enzyme gene polymorphism, plasma levels, and diabetic retinal and renal complications

Diabetes ◽  
1994 ◽  
Vol 43 (3) ◽  
pp. 384-388 ◽  
Author(s):  
M. Marre ◽  
P. Bernadet ◽  
Y. Gallois ◽  
F. Savagner ◽  
T. T. Guyene ◽  
...  
Keyword(s):  
Gene Polymorphism ◽  
Plasma Levels ◽  
Converting Enzyme ◽  
Angiotensin I ◽  
Enzyme Gene ◽  
Angiotensin I Converting Enzyme ◽  
Renal Complications

Differential display competitive polymerase chain reaction: An optimal tool for assaying gene expression

1999 ◽  
Vol 20 (2) ◽  
pp. 230 ◽  
Author(s):  
Marianne Jorgensen ◽  
Maja Bévort ◽  
Thuri S. Kledal ◽  
Brian V. Hansen ◽  
Marlene Dalgaard ◽  
...  
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Differential Display ◽  
Chain Reaction ◽  
Competitive Polymerase Chain Reaction ◽  
Polymerase Chain

scholarly journals Anti-Inflammatory Effects of Abeliophyllum distichum Nakai (Cultivar Okhwang 1) Callus through Inhibition of PI3K/Akt, NF-κB, and MAPK Signaling Pathways in Lipopolysaccharide-Induced Macrophages

Processes ◽  
2021 ◽  
Vol 9 (6) ◽  
pp. 1071
Author(s):  
Tae-Won Jang ◽  
Jae-Ho Park
Keyword(s):  
Gene Expression ◽  
Polymerase Chain Reaction ◽  
Signaling Pathways ◽  
Chain Reaction ◽  
Inos Gene ◽  
Polymerase Chain ◽  
Cox 2 ◽  
Anti Inflammatory ◽  
Abeliophyllum Distichum ◽  
Inos Gene Expression

One of the Korean endemic plants, Abeliophyllum distichum Nakai (Oleaceae), contains acteoside, which is a glycoside exhibiting neuroprotective, anti-inflammation effects and antibacterial capacities. We conducted an investigation on the effects of the callus of A. distichum (cultivar Okhwang 1, CAO) on pro-inflammatory mediators released following nuclear factor-кB (NF-кB), phosphatidylinositol 3-kinase/Akt (PI3K-Akt) and mitogen-activated protein kinase (MAPK) signal activation in lipopolysaccharide (LPS)-induced RAW 264.7 cells. Immunoblotting was employed to find out the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide (iNOS), and activation of MAPK molecules, NF-κB and Akt. Cytokines, COX-2, and iNOS gene expression were assessed using polymerase chain reaction techniques. Cytokines, COX-2, and iNOS gene expression were assessed using polymerase chain reaction techniques. High-performance liquid chromatography revealed that CAO was rich in acteoside and isoacteoside. As a result, CAO inhibited the generation of NO, cytokines, COX-2, and iNOS expression. Further, translocation to the nuclear of NF-κB p65 and degradation of the inhibitor of NF-кB (IкB) were alleviated by suppressing phosphorylation. Additionally, CAO significantly impacted MAPK pathway activation by potentially reducing phosphorylation of MAPKs. These results indicate that the anti-inflammatory effect of CAO is mediated via the inhibition of MAPK, PI3K/Akt, and NF-κB signaling pathways, probably via glycosides, phenolics, and flavonoids bioactivity derived from plants. CAO can serve as a potential anti-inflammatory agent, which alleviates inflammation factors and act through specific cell signaling pathways.

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