scholarly journals Na,K-ATPase in diabetic rat small intestine. Changes at protein and mRNA levels and role of glucagon.

1994 ◽  
Vol 93 (6) ◽  
pp. 2725-2731 ◽  
Author(s):  
K Barada ◽  
C Okolo ◽  
M Field ◽  
N Cortas
Keyword(s):  
Small Intestine ◽  
Diabetic Rat ◽  
Mrna Levels ◽  
Rat Small Intestine

Transcriptional regulation of intestinal hydrolase biosynthesis during postnatal development in rats

1994 ◽  
Vol 267 (4) ◽  
pp. G584-G594 ◽  
Author(s):  
S. D. Krasinski ◽  
G. Estrada ◽  
K. Y. Yeh ◽  
M. Yeh ◽  
P. G. Traber ◽  
...  
Keyword(s):  
Small Intestine ◽  
Transcriptional Control ◽  
Mrna Levels ◽  
Rat Small Intestine ◽  
Rnase Protection ◽  
Reciprocal Regulation ◽  
Adult Rat ◽  
Microvillus Membrane ◽  
Specific Activities ◽  
Respective Protein

Lactase-phlorizin hydrolase (LPH) and sucrase-isomaltase (SI) are intestine-specific microvillus membrane hydrolases whose specific activities demonstrate reciprocal regulation during development but whose mechanisms of regulation have not been fully defined. To investigate transcriptional control of these two proteins, the rat LPH and SI genes were cloned, and antisense probes for preprocessed mRNAs (pre-mRNAs) were developed from intron sequence. LPH mRNA, as measured by quantitative ribonuclease (RNase) protection assays, was abundant before weaning and decreased two- to fourfold during weaning, whereas SI mRNA was first detected 14 days after birth and increased rapidly to abundant levels by age 28 days. LPH and SI pre-mRNA levels paralleled those of their respective mRNAs. LPH transcriptional rate declined during weaning, whereas that of SI increased during this time as determined by RNase protection assays of pre-mRNAs and nuclear run-on assays. In the adult rat, LPH mRNA was restricted to the jejunum and proximal ileum, whereas SI mRNA was detected throughout the small intestine, a pattern regulated by transcriptional rate as confirmed by nuclear run-on assays. Lactase and sucrase specific activities correlated well with their respective protein and mRNA concentrations in all experiments. We conclude that gene transcription plays a major role in the developmental and horizontal regulation of LPH and SI biosynthesis and that these two genes are regulated differently in rat small intestine.

Mechanisms of endothelin-1 induced mucosal dysfunction in the rat small intestine: Role of leukocytes

1998 ◽  
Vol 114 ◽  
pp. A384
Author(s):  
B. Karakoyun ◽  
T. Coskun ◽  
A. Bozkurt ◽  
B.Ç. Yegen ◽  
M. Yüksel ◽  
...  
Keyword(s):  
Small Intestine ◽  
Rat Small Intestine ◽  
Endothelin 1

ALTERED NEURAL CONTROL OF EPITHELIAL FUNCTION IN DIABETIC RAT SMALL INTESTINE

1998 ◽  
Vol 26 (5) ◽  
pp. 557
Author(s):  
E. Mendez ◽  
J. Nealon ◽  
V. Piñeiro-Carrero ◽  
S. Solomon ◽  
T. Shea-Donohue
Keyword(s):  
Small Intestine ◽  
Neural Control ◽  
Diabetic Rat ◽  
Rat Small Intestine

scholarly journals Orange Juice and Its Component, Hesperidin, Decrease the Expression of Multidrug Resistance-Associated Protein 2 in Rat Small Intestine and Liver

2011 ◽  
Vol 2011 ◽  
pp. 1-8 ◽  
Author(s):  
Minoru Watanabe ◽  
Naoki Matsumoto ◽  
Yuko Takeba ◽  
Toshio Kumai ◽  
Masami Tanaka ◽  
...  
Keyword(s):  
Small Intestine ◽  
Multidrug Resistance ◽  
Orange Juice ◽  
Tap Water ◽  
Pharmacokinetic Parameters ◽  
Mrna Levels ◽  
Rat Small Intestine ◽  
Sprague Dawley ◽  
Water Ingestion ◽  
Sprague Dawley Rats

We investigated the effects of orange juice (OJ) or hesperidin, a component of OJ, on the pharmacokinetics of pravastatin (PRV) and the expression of both protein and mRNA of multidrug resistance-associated protein 2 (Mrp2) in the rat small intestine and liver. Eight-week-old male Sprague-Dawley rats were used in this study. OJ or a 0.079% hesperidin suspension was administered orally for 2 days. Tap water was given as a control. A single dose of PRV at 100 mg/kg p.o. was administered after 2 days of OJ, hesperidin, or tap water ingestion. The AUC, , andt1/2values of PRV were significantly increased in OJ group. Mrp2 protein and mRNA levels in the small intestine and liver, respectively, were significantly decreased after the ingestion of OJ. The same results were obtained with hesperidin. These results suggest that the changes in PRV pharmacokinetic parameters and the decrease in Mrp2 expression caused by OJ are due to hesperidin in the juice.

Evidence of stereoselective and segmental-dependant transport of chiral antidiabetic drug nateglinide along the rat small intestine: possible role of efflux transporters

2012 ◽  
Vol 22 (5) ◽  
pp. 2403-2410 ◽  
Author(s):  
Srinivas Maddi ◽  
Shravan Kumar Yamsani ◽  
Adukondalu Devandla ◽  
Gerhard Scriba ◽  
Madhusudan Rao Yamsani
Keyword(s):  
Small Intestine ◽  
Efflux Transporters ◽  
Rat Small Intestine ◽  
Antidiabetic Drug

The Role of Tumor Necrosis Factor-α and Interleukin-1β in Ischemia-Reperfusion Injury of the Rat Small Intestine

2001 ◽  
Vol 99 (1) ◽  
pp. 134-141 ◽  
Author(s):  
Seiichiro Yamamoto ◽  
Minoru Tanabe ◽  
Go Wakabayashi ◽  
Motohide Shimazu ◽  
Koshi Matsumoto ◽  
...  
Keyword(s):  
Small Intestine ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Ischemia Reperfusion Injury ◽  
Ischemia Reperfusion ◽  
Rat Small Intestine ◽  
Factor Α ◽  
Necrosis Factor

Apolipoprotein A-IV synthesis in rat intestine: regulation by dietary triglyceride

1987 ◽  
Vol 252 (5) ◽  
pp. G662-G666 ◽  
Author(s):  
T. F. Apfelbaum ◽  
N. O. Davidson ◽  
R. M. Glickman
Keyword(s):  
Protein Synthesis ◽  
Small Intestine ◽  
Total Protein ◽  
Mrna Levels ◽  
Rat Small Intestine ◽  
Rat Intestine ◽  
Apolipoprotein A ◽  
Rat Enterocytes

Apolipoprotein A-IV (apoA-IV) synthesis rates were measured in vivo in rat enterocytes by immunoprecipitation after administration of [3H]leucine into in situ loops of jejunum and ileum. Basal apoA-IV synthesis rates (percent total protein synthesis) were significantly higher in jejunal enterocytes (2.05 +/- 0.54%) compared with ileal enterocytes (0.48 +/- 0.32%) from the same fasted animals. After an acute triglyceride bolus, significant and sustained elevations of apoA-IV synthesis rates were seen in both jejunal and ileal enterocytes with maximal effects noted at 4-6 h. Animals fed diets containing 30% wt/wt triglyceride as saturated (SF) or polyunsaturated (UF) fats for 6 wk had similarly increased rates of apoA-IV synthesis in jejunal enterocytes with both SF (3.73 +/- 0.83%) and UF (3.33 +/- 0.64%) but no change in ileal enterocytes. By contrast, animals consuming a fat-free diet for 3 wk had jejunal apoA-IV synthesis rates indistinguishable from basal values (2.40 +/- 0.45%). Translatable intestinal mRNA levels for pre-apoA-IV after triglyceride increased in parallel to synthesis rates with a 50% increase in jejunum and a 350% increase in ileum observed at 4-6 h. These results suggest that apoA-IV synthesis by rat small intestine increases in response to acute and chronic dietary triglyceride, is maintained in the absence of dietary triglyceride, and may be under pretranslational control.

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