scholarly journals Identification of two single base substitutions in the UGT1 gene locus which abolish bilirubin uridine diphosphate glucuronosyltransferase activity in vitro.

1994 ◽  
Vol 93 (2) ◽  
pp. 564-570 ◽  
Author(s):  
L T Erps ◽  
J K Ritter ◽  
J H Hersh ◽  
D Blossom ◽  
N C Martin ◽  
...  
Keyword(s):  
Gene Locus ◽  
Uridine Diphosphate ◽  
Single Base ◽  
Uridine Diphosphate Glucuronosyltransferase ◽  
Base Substitutions ◽  
Ugt1 Gene

In vitro assembly of 30S and 70S bacterial ribosomes from 16S RNA containing single base substitutions, insertions, and deletions around the decoding site (C1400)

Biochemistry ◽  
1989 ◽  
Vol 28 (3) ◽  
pp. 1002-1011 ◽  
Author(s):  
Robert Denman ◽  
Carl Weitzmann ◽  
Philip R. Cunningham ◽  
Didier Negre ◽  
Kelvin Nurse ◽  
...  
Keyword(s):  
Single Base ◽  
Insertions And Deletions ◽  
Base Substitutions ◽  
16S Rna ◽  
In Vitro Assembly

Evaluation of the in vitro/in vivo potential of uva ursi to inhibit uridine diphosphate-glucuronosyltransferase isoforms

2017 ◽  
Vol 32 (1) ◽  
pp. S66
Author(s):  
Jung Bae Park ◽  
Seung Jun Lee ◽  
Ji Seon Kim ◽  
Doyun Kim ◽  
Jee Sun Min ◽  
...  
Keyword(s):  
Uridine Diphosphate ◽  
Uridine Diphosphate Glucuronosyltransferase

scholarly journals New inhibitors of glucosylceramide synthase and their effect on cell fate

2014 ◽  
Vol 7 (2) ◽  
pp. 99-104 ◽  
Author(s):  
Katarína Turáková ◽  
Boris Lakatoš ◽  
Andrej Ďuriš ◽  
Daniela Moravčíková ◽  
Dušan Berkeš
Keyword(s):  
Cell Viability ◽  
Cell Fate ◽  
Calcium Transport ◽  
Large Scale ◽  
Process Development ◽  
Pharmaceutical Research ◽  
Pathological Process ◽  
Uridine Diphosphate ◽  
Induction Of Apoptosis

Abstract Glucosylceramide (GlcCer) is an essential glycosylated lipid found in organisms ranging from fungi to mammals. It is composed of a hydrophilic β-linked glucose and a hydrophobic ceramide, with a predominant content of sphingosine in mammals (d18:1). GlcCer is the precursor of a large scale of different glycosphingolipids. This cerebrozide is synthesized from uridine diphosphate-glucose and ceramide by a GlcCer synthase (UDP-glucose:ceramide glucosyltransferase; UGCG, EC 2.4.1.80). GlcCer-based sphingolipids have been identified as important mediators of a variety of cellular functions and their disequilibrium leads to pathological process development and may induce several diseases progression. Therefore, design of UGCG inhibitor represents an important topic for pharmaceutical research. In this paper, we aimed to study effects of newly synthesized derivatives of (±)-threo-1-phenyl-2-palmitoylamino-3-morpholino-1-propanol (PPMP, known UGCG inhibitor) on: i) activity of UGCG in vitro; ii) thymocytes viability; iii) calcium transport through plasma membrane of thymocytes; iv) induction of apoptosis and autophagy in thymocytes. Thymocytes were isolated from thymus of three to seven weeks old mice (ICR strain). The key factors influencing the effect of PPMP analogues were their concentration, chemical structure and incubation time. Derivatives were able to change Ca2+ transport already after 15 min of cultivation, but their effects on cell viability were manifested at least after 12 h of cultivation. Four from fifteen studied compounds affected UGCG activity after four hour lasting cultivation, - but without correlation with data relating to effects on calcium transport and/or cell viability. Most potent UGCG inhibitor was chosen and applied for induction of apoptosis and autophagy in thymocytes. This inhibitor induced typical DNA fragmentation and upregulation of LC3B protein as autophagy marker, after 2 h and 4 h cultivation, respectively.

A single Saccharomyces cerevisiae upstream activation site (UAS1) has two distinct regions essential for its activity

1986 ◽  
Vol 6 (12) ◽  
pp. 4690-4696
Author(s):  
B Lalonde ◽  
B Arcangioli ◽  
L Guarente
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Mutations ◽  
Site Directed Mutagenesis ◽  
Single Base ◽  
Protein Factor ◽  
Fold Reduction ◽  
Dependent Protein ◽  
Substitution Mutations ◽  
Activation Site

Several site-directed mutagenesis regimens were used to generate single- and multiple-base substitutions in the upstream activation site UAS1 of the Saccharomyces cerevisiae CYC1 gene. Mutations resulting in large reductions in activity of the site lie in two distinct regions. Six single-base changes in a region A, between -288 and -285, all resulted in a 15-fold reduction in activity. Synthetic sites built up solely of multimers of the -289 to -285 sequence ACCGA behaved as carbon catabolite-sensitive UASs. In addition, substitution mutations in a second region, at nucleotides -266 and -265, virtually eliminated UAS1 activity. These mutations abolished the binding of a heme-dependent protein factor in vitro. Thus, UAS1 contains two essential regions both of which are required for its activity.

The AAUAAA sequence is required both for cleavage and for polyadenylation of simian virus 40 pre-mRNA in vitro

1986 ◽  
Vol 6 (7) ◽  
pp. 2317-2323
Author(s):  
D Zarkower ◽  
P Stephenson ◽  
M Sheets ◽  
M Wickens
Keyword(s):  
Hela Cell ◽  
Simian Virus 40 ◽  
Nuclear Extract ◽  
Simian Virus ◽  
Polyadenylation Site ◽  
Single Base ◽  
Cleavage And Polyadenylation ◽  
Cell Nuclear Extract

The sequence AAUAAA is found near the polyadenylation site of eucaryotic mRNAs. This sequence is required for accurate and efficient cleavage and polyadenylation of pre-mRNAs in vivo. In this study we show that synthetic simian virus 40 late pre-mRNAs are cleaved and polyadenylated in vitro in a HeLa cell nuclear extract, and that cleavage in vitro is abolished by each of four different single-base changes in AAUAAA. In this same extract, precleaved RNAs (RNAs with 3' termini at the polyadenylation site) are efficiently polyadenylated. This in vitro polyadenylation reaction also requires the AAUAAA sequence.

scholarly journals Inter- and intraspecific variation of chloroplast mini- and microsatellites DNA in the four closed related Acacia species

2015 ◽  
Vol 19 (1) ◽  
pp. 1
Author(s):  
AYPBC Widyatmoko ◽  
Susumu Shiraishi
Keyword(s):  
Intraspecific Variation ◽  
Sequence Information ◽  
Single Base ◽  
Coding Regions ◽  
Acacia Species ◽  
Base Substitutions ◽  
Microsatellites Dna

Mini- and microsatellites of four Acacia species, A. aulacocarpa, A. auriculiformis, A. crassicarpa and A.mangium were investigated on four non-coding regions of cpDNA, the intron of trnL, and the intergenicspacers of trnL - trnP, trnD - trnY, and trnP – trnW. Nine single base substitutions and six informative miniandmicrosatellites were detected in the the four cpDNA non-coding regions. Based on the substitutionsand mini- and microsatellites, ten cpDNA haplotypes (A - J) could be distinguished. Acacia auriculiformispossessed fi ve haplotypes, A. aulacocarpa, four haplotypes, and A. crassicarpa, three haplotypes. All samplesof A. mangium possessed the same haplotype. Mini- and microsatellites recognized in this study can beused for species identifi cation of the four Acacia species. The ten haplotypes could divided the four speciesinto 2 groups, A. aulacocarpa-A.crassicarpa group and A. auriculiformis-A. mangium group. By developing thePCR-based markers based on the sequence information, many experiments can be carried out for the Acaciaimprovement programs.

Uridine diphosphate glucose synthase from calf liver. Determinants of enzyme activity in vitro

Biochemistry ◽  
1975 ◽  
Vol 14 (25) ◽  
pp. 5445-5450 ◽  
Author(s):  
Peter J. Roach ◽  
Kenneth R. Warren ◽  
Daniel E. Atkinson
Keyword(s):  
Enzyme Activity ◽  
Uridine Diphosphate ◽  
Uridine Diphosphate Glucose ◽  
Diphosphate Glucose
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