scholarly journals Molecular mimicry in primary biliary cirrhosis. Evidence for biliary epithelial expression of a molecule cross-reactive with pyruvate dehydrogenase complex-E2.

1993 ◽  
Vol 91 (6) ◽  
pp. 2653-2664 ◽  
Author(s):  
J Van de Water ◽  
J Turchany ◽  
P S Leung ◽  
J Lake ◽  
S Munoz ◽  
...  
Keyword(s):  
Primary Biliary Cirrhosis ◽  
Pyruvate Dehydrogenase ◽  
Molecular Mimicry ◽  
Pyruvate Dehydrogenase Complex ◽  
Biliary Cirrhosis ◽  
Dehydrogenase Complex

Human Pyruvate Dehydrogenase Complex as An Autoantigen in Primary Biliary Cirrhosis

1993 ◽  
Vol 85 (3) ◽  
pp. 289-293 ◽  
Author(s):  
Jeremy M. Palmer ◽  
Margaret F. Bassendine ◽  
Oliver F. W. James ◽  
Stephen J. Yeaman
Keyword(s):  
Primary Biliary Cirrhosis ◽  
Pyruvate Dehydrogenase ◽  
Pyruvate Dehydrogenase Complex ◽  
Heart Tissue ◽  
Immune Reactivity ◽  
Biliary Cirrhosis ◽  
Antimitochondrial Antibodies ◽  
Future Work ◽  
Dehydrogenase Complex

1. The sera of more than 90% of patients with primary biliary cirrhosis contain antimitochondrial antibodies which react with the E2 component of the pyruvate dehydrogenase complex, identified as the major autoantigen in primary biliary cirrhosis. All previous studies in this area have utilized protein derived from animal tissue or have used recombinant human pyruvate dehydrogenase complex E2 expressed in Escherichia coli. 2. We report the preparation and characterization of native pyruvate dehydrogenase complex and pyruvate dehydrogenase complex E2 from human heart tissue and its application in studies of immune reactivity with the sera of patients with primary biliary cirrhosis. 3. The immune reactivity of sera from patients with primary biliary cirrhosis versus the bovine and human E2/X components of pyruvate dehydrogenase complex was indistinguishable in both immunoblotting and the more sensitive e.l.i.s.a. 4. These findings suggest that the reactivity of sera from patients with primary biliary cirrhosis against the major autoantigen of the disease is a property of that antigen, independent of its human or bovine origin. Furthermore, this justifies the use of bovine pyruvate dehydrogenase complex in past and future work on primary biliary cirrhosis antibody reactivity.

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