scholarly journals Diversity of airway epithelial cell targets for in vivo recombinant adenovirus-mediated gene transfer.

1993 ◽  
Vol 91 (1) ◽  
pp. 225-234 ◽  
Author(s):  
A Mastrangeli ◽  
C Danel ◽  
M A Rosenfeld ◽  
L Stratford-Perricaudet ◽  
M Perricaudet ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Epithelial Cell ◽  
Airway Epithelial Cell ◽  
Recombinant Adenovirus ◽  
Airway Epithelial

scholarly journals The Function of Ophiocordyceps sinensis in Airway Epithelial Cell Senescence in a Rat COPD Model

2018 ◽  
Vol 2018 ◽  
pp. 1-9
Author(s):  
Xiaohui Ma ◽  
Xingai Jiao ◽  
Jinxiang Wu ◽  
Jiping Zhao ◽  
Yurong Xu ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Quantitative Pcr ◽  
Airway Epithelial Cell ◽  
Cell Senescence ◽  
Airway Epithelial ◽  
Airway Epithelia ◽  
Real Time Quantitative Pcr ◽  
Ophiocordyceps Sinensis

Ophiocordyceps sinensis (O. sinensis) seems to be able to alleviate airway epithelial cell senescence in chronic obstructive pulmonary disease (COPD). The objective of the study is to evaluate the effect of O. sinensis on airway epithelial senescence in the COPD model both in vitro and in vivo. We observed the expression of P16 and P21 in the airway epithelia of 30 patients with COPD. The optimal concentration of O. sinensis and exposure time of the cigarette smoke extract (CSE) were determined in vitro, and senescence-associated β-galactosidase (SA-β-gal) and 5-bromodeoxyuridine (BrdU) were used to evaluate the senescence and proliferation of human bronchial epithelial (16HBE) cells pretreated with O. sinensis by staining kits. COPD model rats were treated with O. sinensis at various concentrations to determine the changes in P16 and P21 expression in airway epithelial tissues. It was found that the expression levels of P16 and P21 were higher in the airway epithelia of COPD patients than those in the control group based on immunohistochemical staining, real-time quantitative PCR, and western blotting. The CSE could induce 16HBE cell senescence, and O. sinensis could alleviate CSE-induced senescence and promote the proliferation of 16HBE cells. The expression levels of P16 and P21 were also higher in the airway epithelia of COPD model rats; however, the levels of P16 and P21 in the groups treated with all concentrations of O. sinensis were obviously lower than those in the COPD model group based on real-time quantitative PCR and western blotting. In conclusion, the CSE can induce airway epithelium senescence, and O. sinensis can inhibit CSE-induced cellular senescence, both in vitro and in vivo.

Growth and differentiation of mouse tracheal epithelial cells: selection of a proliferative population

2002 ◽  
Vol 283 (6) ◽  
pp. L1315-L1321 ◽  
Author(s):  
Yingjian You ◽  
Edward J. Richer ◽  
Tao Huang ◽  
Steven L. Brody
Keyword(s):  
Gene Expression ◽  
Epithelial Cell ◽  
Epithelial Cells ◽  
Airway Epithelial Cell ◽  
Airway Epithelial ◽  
Tracheal Epithelial Cells ◽  
Proliferation And Differentiation ◽  
Tracheal Epithelial

Highly regulated programs for airway epithelial cell proliferation and differentiation during development and repair are often disrupted in disease. These processes have been studied in mouse models; however, it is difficult to isolate and identify epithelial cell-specific responses in vivo. To investigate these processes in vitro, we characterized a model for primary culture of mouse tracheal epithelial cells. Small numbers of cells seeded at low density (7.5 × 104 cells/cm2) rapidly proliferated and became polarized. Subsequently, supplemented media and air-liquid interface conditions resulted in development of highly differentiated epithelia composed of ciliated and nonciliated cells with gene expression characteristic of native airways. Genetically altered or injured mouse tracheal epithelial cells also reflected in vivo patterns of airway epithelial cell gene expression. Passage of cells resulted in continued proliferation but limited differentiation after the first passage, suggesting that transit-amplifying cell populations were present but with independent programs for proliferation and differentiation. This approach provides a high-fidelity in vitro model for evaluation of gene regulation and expression in mouse airway epithelial cells.

Isolation of rodent airway epithelial cell proteins facilitates in vivo proteomics studies of lung toxicity

2004 ◽  
Vol 286 (2) ◽  
pp. L399-L410 ◽  
Author(s):  
Åsa M. Wheelock ◽  
Lu Zhang ◽  
Mai-Uyen Tran ◽  
Dexter Morin ◽  
Sharron Penn ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Airway Epithelial Cell ◽  
Standard Procedure ◽  
Airway Epithelial ◽  
Clara Cells ◽  
Two Dimensional Gel Electrophoresis ◽  
Recent Developments ◽  
Specific Proteins ◽  
Cell Phenotypes

Recent developments in genomics, proteomics, and metabolomics hold substantial promise for understanding cellular responses to toxicants. Gene expression profiling is now considered standard procedure, but numerous publications reporting a lack of correlation between mRNA and protein expression emphasize the importance of conducting parallel proteomics studies. The cellular complexity of the lung presents great challenges for in vivo proteomics, and improved isolation methods for proteins from specific lung cell phenotypes are required. To address this issue, we have developed a novel method for isolation of rodent airway epithelial cell proteins that facilitates in vivo proteomics studies of two target-cell pheno-types of the lung, Clara cells and ciliated cells. The airway epithelial cell proteins are reproducibly solubilized, leaving the underlying basement membrane and smooth muscle intact as shown by histopathological analyses. The method yields epithelial cell-specific proteins in fivefold higher concentrations and reduces the yield of nonepithelial cell proteins 13-fold compared with homogenates from microdissected airways. In addition, 36% more protein spots were detectable by two-dimensional gel electrophoresis.

scholarly journals Apical barriers to airway epithelial cell gene transfer with amphotropic retroviral vectors

Gene Therapy ◽  
2002 ◽  
Vol 9 (14) ◽  
pp. 922-931 ◽  
Author(s):  
G Wang ◽  
G Williams ◽  
H Xia ◽  
M Hickey ◽  
J Shao ◽  
...  
Keyword(s):  
Gene Transfer ◽  
Epithelial Cell ◽  
Airway Epithelial Cell ◽  
Retroviral Vectors ◽  
Airway Epithelial ◽  
Cell Gene
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