scholarly journals Defective in vitro growth of the hemopoietic progenitor cells in the acquired immunodeficiency syndrome.

1987 ◽  
Vol 80 (2) ◽  
pp. 286-293 ◽  
Author(s):  
C C Stella ◽  
A Ganser ◽  
D Hoelzer
Keyword(s):  
Progenitor Cells ◽  
Acquired Immunodeficiency Syndrome ◽  
In Vitro Growth ◽  
Acquired Immunodeficiency ◽  
Immunodeficiency Syndrome ◽  
Hemopoietic Progenitor ◽  
Hemopoietic Progenitor Cells

Regulatory action of prolactin on the in vitro growth of CD34+ve human hemopoietic progenitor cells

1995 ◽  
Vol 163 (2) ◽  
pp. 221-231 ◽  
Author(s):  
Graziella Bellone ◽  
Massimo Geuna ◽  
Anna Carbone ◽  
Stefania Silvestri ◽  
Robin Foa ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Regulatory Action ◽  
In Vitro Growth ◽  
Hemopoietic Progenitor ◽  
Hemopoietic Progenitor Cells

Rapid Death of Adoptively Transferred T Cells in Acquired Immunodeficiency Syndrome

Blood ◽  
1999 ◽  
Vol 93 (5) ◽  
pp. 1506-1510 ◽  
Author(s):  
Rusung Tan ◽  
Xiaoning Xu ◽  
Graham S. Ogg ◽  
Pokrath Hansasuta ◽  
Tao Dong ◽  
...  
Keyword(s):  
T Cell ◽  
Adoptive Transfer ◽  
Acquired Immunodeficiency Syndrome ◽  
Mononuclear Cells ◽  
Virus Load ◽  
Acquired Immunodeficiency ◽  
Peptide Complexes ◽  
Immunodeficiency Syndrome

Human immunodeficiency virus (HIV)-specific cytotoxic T lymphocytes (CTL) probably play the major role in controlling HIV replication. However, the value of adoptive transfer of HIV-specific CTL expanded in vitro to HIV+ patients has been limited: this contrasts with the success of CTL therapy in treating or preventing Epstein-Barr virus and cytomegalovirus disease after bone marrow transplantation (BMT). We investigated the fate of expanded HIV-specific CTL clones in vivo following adoptive transfer to a patient with acquired immunodeficiency syndrome (AIDS). Two autologous CTL clones specific for HIV Gag and Pol were expanded to large numbers (>109) in vitro and infused into an HIV-infected patient whose viral load was rising despite antiretroviral therapy. The fate of one clone was monitored by staining peripheral blood mononuclear cells (PBMCs) with T-cell receptor–specific tetrameric major histocompatibility complex (MHC)-peptide complexes. Although the CTL transfer was well tolerated, there were no significant changes in CD4 and CD8 lymphocyte counts and virus load. By tracking an infused clone using soluble MHC-peptide complexes, we show that cells bearing the Gag-specific T-cell receptors were rapidly eliminated within hours of infusion through apoptosis. Thus, the failure of adoptively transferred HIV-specific CTL to reduce virus load in AIDS may be due to rapid apoptosis of the infused cells, triggered by a number of potential mechanisms. Further trials of adoptive transfer of CTL should take into account the susceptibility of infused cells to in vivo apoptosis.

scholarly journals Production of and in vitro response to interleukin 2 in the acquired immunodeficiency syndrome.

1985 ◽  
Vol 76 (5) ◽  
pp. 1959-1964 ◽  
Author(s):  
H W Murray ◽  
K Welte ◽  
J L Jacobs ◽  
B Y Rubin ◽  
R Mertelsmann ◽  
...  
Keyword(s):  
Acquired Immunodeficiency Syndrome ◽  
Interleukin 2 ◽  
In Vitro Response ◽  
Acquired Immunodeficiency ◽  
Immunodeficiency Syndrome

Activation of monocyte-mediated tumoricidal activity in patients with acquired immunodeficiency syndrome.

1985 ◽  
Vol 3 (7) ◽  
pp. 1005-1012 ◽  
Author(s):  
E S Kleinerman ◽  
L M Ceccorulli ◽  
L A Zwelling ◽  
T Twilley ◽  
R B Herberman ◽  
...  
Keyword(s):  
Acquired Immunodeficiency Syndrome ◽  
Mononuclear Cells ◽  
Human Tumor ◽  
Target Cells ◽  
Aids Patients ◽  
Tumoricidal Activity ◽  
Ifn Gamma ◽  
Acquired Immunodeficiency ◽  
Immunodeficiency Syndrome

The purpose of these studies was to determine whether peripheral blood monocytes from acquired immunodeficiency syndrome (AIDS) patients with Kaposi's sarcoma could be activated to lyse human tumor target cells in vitro. Monocytes were isolated and incubated for 24 hours in vitro with either medium (control), a crude mitogen-induced lymphokine preparation (MAF), or endotoxin before the addition of [125I]IUdR-labeled A375 melanoma target cells. Cytolysis was determined 72 hours later. Twelve (100%) of 12 patients tested had monocyte-mediated cytotoxicity values that were comparable to those of normal individuals. Recombinant human gamma interferon (IFN gamma) activated both normal and AIDS monocyte-mediated tumoricidal function only when combined with lypopolysaccharide (LPS). In addition, mononuclear cells from ten AIDS patients were also tested for their ability to secrete MAF and IFN gamma in response to a mitogenic stimulus. Lymphokines generated from all ten patients contained substantial amounts of IFN gamma (100 to 2,500 U/mL); however, three of these ten lymphokine preparations failed to activate normal monocytes to lyse tumor cells. These results suggest that monocyte-mediated tumoricidal function of AIDS patients is intact and thus suggest new approaches for the therapy of AIDS.

scholarly journals Antibody stimulation of hemopoietic progenitor cells

Blood ◽  
1985 ◽  
Vol 65 (4) ◽  
pp. 869-876 ◽  
Author(s):  
RM Crapper ◽  
JW Schrader
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Bone Marrow Cells ◽  
Exchange Chromatography ◽  
Staphylococcal Protein A ◽  
Igg Antibodies ◽  
Hemopoietic Progenitor ◽  
Hemopoietic Progenitor Cells ◽  
Stimulation Of

Abstract Antisera were raised by immunizing rabbits with cloned lines of murine hemopoietic progenitor cells (P cells) that depended on the presence of a specific hemopoietic growth factor, persisting cell-stimulating factor (PSF), for their growth and survival. The unabsorbed antiserum was inhibitory, but after absorption with murine spleen cells and the mastocytoma, P815, significant stimulation of both P cell growth and thymidine incorporation was evident. IgG antibodies isolated from the antiserum by staphylococcal protein A chromatography or further purified by diethylaminoethyl anion exchange chromatography, ammonium sulphate precipitation, and gel filtration using Sephacryl S-300 were responsible for the stimulation. The absorbed antiserum promoted the survival of normal murine bone marrow cells in liquid culture over a four-day period, and the inclusion of IgG antibodies in agar cultures of normal bone marrow promoted the in vitro survival, over a 48-hour period, of cells capable of subsequently generating, in the presence of a source of PSF, colonies of neutrophils, macrophages, and megakaryocytes. It is postulated that the antibodies act by stimulating the PSF receptor on both the factor-dependent cell lines and normal myeloid progenitor cells.

scholarly journals Near-optimal Conditions for the In Vitro Culture of Hemopoietic Progenitor Cells in Bone Marrow from the Rat

2009 ◽  
Vol 37 (2) ◽  
pp. 170-174 ◽  
Author(s):  
Gemma Molyneux ◽  
Sian Rizzo ◽  
John Turton ◽  
Parvinder Phul ◽  
Frances Gibson
Keyword(s):  
Bone Marrow ◽  
In Vitro Culture ◽  
Progenitor Cells ◽  
Optimal Conditions ◽  
Hemopoietic Progenitor ◽  
Hemopoietic Progenitor Cells
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