scholarly journals Effects of recombinant tumor necrosis factor on proliferation and differentiation of leukemic and normal hemopoietic cells in vitro. Relationship to cell surface receptor.

1986 ◽  
Vol 78 (6) ◽  
pp. 1694-1700 ◽  
Author(s):  
C Peetre ◽  
U Gullberg ◽  
E Nilsson ◽  
I Olsson
Keyword(s):  
Tumor Necrosis Factor ◽  
Cell Surface ◽  
Tumor Necrosis ◽  
Cell Surface Receptor ◽  
Hemopoietic Cells ◽  
Proliferation And Differentiation ◽  
Surface Receptor ◽  
Recombinant Tumor Necrosis Factor ◽  
Necrosis Factor

scholarly journals Antibodies against amino acids 1-15 of tumor necrosis factor block its binding to cell-surface receptor.

1987 ◽  
Vol 84 (24) ◽  
pp. 8829-8833 ◽  
Author(s):  
S. H. Socher ◽  
M. W. Riemen ◽  
D. Martinez ◽  
A. Friedman ◽  
J. Tai ◽  
...  
Keyword(s):  
Amino Acids ◽  
Tumor Necrosis Factor ◽  
Cell Surface ◽  
Tumor Necrosis ◽  
Cell Surface Receptor ◽  
Surface Receptor ◽  
Necrosis Factor

Effects of recombinant tumor necrosis factor (rHuTNFα)on human neutrophils and monocytes: In vitro, ex vivo and in vivo

2009 ◽  
Vol 43 (4) ◽  
pp. 286-296 ◽  
Author(s):  
E. Schell-Frederick ◽  
T. Tepass ◽  
G. Lorscheidt ◽  
M. Pfreundschuh ◽  
M. Schaadt ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Ex Vivo ◽  
Human Neutrophils ◽  
Recombinant Tumor Necrosis Factor ◽  
Necrosis Factor

In vitro effects of tumor necrosis factor-α on human thyroid follicular cells

1992 ◽  
Vol 127 (3) ◽  
pp. 220-225 ◽  
Author(s):  
Ulrich Deuss ◽  
Massimo Buscema ◽  
Heike Schumacher ◽  
Werner Winkelmann
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Human Thyroid ◽  
Thyroid Follicular Cells ◽  
Factor Α ◽  
Higher Doses ◽  
Recombinant Tumor Necrosis Factor ◽  
Necrosis Factor

Tumor necrosis factor-α is assumed to be an important mediator in thyroid autoimmunity. In the present study we have shown that human thyrocytes possess a single specific binding site for recombinant tumor necrosis factor-α with an average of 9,300 receptors/cell (Kd = 1.9 · 10−10 mol). The effects of the cytokine on thyroid cell proliferation were assessed by 3H-thymidine uptake as well as by the protein and DNA content of cell monolayers. Low dose tumor necrosis factor-α resulted in a moderate stimulation of cell proliferation with an increase of 3H-thymidine incorporation from 44,613±7,989 cpm under basal conditions to 63,326±6,822 cpm after 100 U/l tumor necrosis factor-α (p <0.01). Higher doses of the cytokine were less effective. On average, bTSH stimulated cAMP production of human thyrocytes was significantly augmented after preincubation with recombinant tumor necrosis factor-α. The maximum effect was observed after 1,000 U/l tumor necrosis factor-α (281.5±107.0 vs 114.5±33.6 fmol cAMP/μg protein under basal conditions; p<0.05), whereas higher doses of the cytokine were again less effective. This phenomenon could at least partly be explained by a cytokine-mediated downregulation of tumor necrosis factor-α binding. We conclude that in vitro tumor necrosis factor-α modulates in addition to its well known synergistic effect on interferon-γ induced HLA class II expression the function and proliferation of human thyroid follicular cells as well. These effects are mediated via specific cell surface receptors.

Reduced [14C]-methionine uptake and fecundity in Schistosoma mansoni females treated with recombinant tumor necrosis factor α in vitro

2017 ◽  
Vol 62 (1) ◽  
Author(s):  
M.A. Haseeb ◽  
Raag Agrawal ◽  
Bernard Fried
Keyword(s):  
Tumor Necrosis Factor ◽  
Schistosoma Mansoni ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Methionine Uptake ◽  
Factor Α ◽  
Recombinant Tumor Necrosis Factor ◽  
Necrosis Factor

AbstractRecombinant human TNFα (rhTNFα) has previously been shown to reduce fecundity in

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