scholarly journals Chronic caffeine ingestion sensitizes the A1 adenosine receptor-adenylate cyclase system in rat cerebral cortex.

1986 ◽  
Vol 77 (1) ◽  
pp. 222-227 ◽  
Author(s):  
R M Green ◽  
G L Stiles
Keyword(s):  
Cerebral Cortex ◽  
Adenylate Cyclase ◽  
Adenosine Receptor ◽  
Rat Cerebral Cortex ◽  
Adenylate Cyclase System ◽  
A1 Adenosine Receptor ◽  
Caffeine Ingestion ◽  
Chronic Caffeine

scholarly journals Inhibition of adenylate cyclase in rat brain synaptosomal membranes by GTP and phenylisopropyladenosine is enhanced in hypothyroidism

1989 ◽  
Vol 263 (3) ◽  
pp. 829-835 ◽  
Author(s):  
D Mazurkiewicz ◽  
E D Saggerson
Keyword(s):  
Adenylate Cyclase ◽  
Rat Brain ◽  
Adenosine Receptor ◽  
Receptor Agonist ◽  
Adenylate Cyclase System ◽  
Synaptosomal Membranes ◽  
A1 Adenosine Receptor ◽  
Adenosine Receptor Agonist ◽  
Coupling Protein ◽  
The Brain

1. Synaptosomal membranes were isolated from rats made hypothyroid by treatment with propylthiouracil and a low iodine diet. 2. When assayed in the presence of 100 mM-Na+, inhibition of forskolin-stimulated adenylate cyclase by GTP was enhanced in membranes from hypothyroid animals. 3. Hypothyroidism also enhanced inhibition of adenylate cyclase by phenylisopropyladenosine (with 100 mM-Na+ and 10 microM-GTP present). 4. Hypothyroidism did not increase binding of the A1 adenosine receptor agonist phenylisopropyladenosine to synaptosomal membranes; rather, the maximum binding was slightly decreased without any change in the KD. 5. The effect of GTP in modifying the displacement of the antagonist [3H]diethylphenylxanthine from synaptosomal membranes by unlabelled phenylisopropyladenosine was more pronounced in the hypothyroid state. 6. These findings are consistent with hypothyroidism causing modification of the brain adenylate cyclase system at the level of the coupling protein Gi.

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