scholarly journals Mechanism of complement-mediated activation of human blood platelets in vitro: comparison of normal and paroxysmal nocturnal hemoglobinuria platelets.

1977 ◽  
Vol 59 (2) ◽  
pp. 360-368 ◽  
Author(s):  
R H Dixon ◽  
W F Rosse
Keyword(s):  
Human Blood ◽  
Paroxysmal Nocturnal Hemoglobinuria ◽  
Blood Platelets ◽  
Human Blood Platelets ◽  
In Vitro Comparison

scholarly journals Survival and recovery of human platelets stored for five days in a non- plasma medium

Blood ◽  
1986 ◽  
Vol 67 (3) ◽  
pp. 672-675 ◽  
Author(s):  
GA Adams ◽  
SD Swenson ◽  
G Rock
Keyword(s):  
Platelet Aggregation ◽  
Human Blood ◽  
Blood Platelets ◽  
Human Platelets ◽  
Plasma Medium ◽  
Release Reaction ◽  
In Vivo Recovery ◽  
Human Blood Platelets

Abstract Human blood platelets were stored for five days as concentrates in 60 mL of: (a) plasma; (b) non-plasma medium with anticoagulant; and (c) non-plasma medium without anticoagulant. All preparations were equally functional when tested for platelet aggregation and release reaction in response to single agonist or synergistic pairs of agonists in vitro. Platelets stored in non-plasma medium with anti-coagulant had lower kallikrein, fibrino(gen)peptide A, lactate, and beta-thromboglobulin than did plasma controls after five days. In vivo recovery and survival of platelets stored in non-plasma medium with anticoagulant were 51.2% +/- 4.3% and 8.7 +/- 0.3 days, respectively, which were not statistically different from plasma controls of 39.2% +/- 4.9% and 7.2 +/- 0.8 days, respectively. It is concluded that platelets can be stored for five days in a non-plasma medium and still have good in vivo recoveries and survivals.

Effects of ethanol and of other factors on ADP-induced aggregation of human blood platelets in vitro

1980 ◽  
Vol 20 (4) ◽  
pp. 405-415 ◽  
Author(s):  
Ronald P. Quintana ◽  
Andrew Lasslo ◽  
Marion Dugdale ◽  
Lisa L. Goodin ◽  
Eric F. Burkhardt
Keyword(s):  
Human Blood ◽  
Blood Platelets ◽  
Induced Aggregation ◽  
Human Blood Platelets

Adsorption of Carbon-14 Dextran to Human Blood Platelets and Red Blood Cells, in Vitro

Vox Sanguinis ◽  
1957 ◽  
Vol 2 (2) ◽  
pp. 104-109 ◽  
Author(s):  
S. ROTHMAN ◽  
E. ADELSON ◽  
A. SCHWEBEL ◽  
R. D. LANGDELL
Keyword(s):  
Red Blood Cells ◽  
Human Blood ◽  
Blood Cells ◽  
Blood Platelets ◽  
Carbon 14 ◽  
Human Blood Platelets

The Active Serotonin Uptake and the Hypotonic Shock Response of Human Blood Platelets

1974 ◽  
Vol 32 (02/03) ◽  
pp. 391-404
Author(s):  
M. R Hardeman ◽  
Carina J. L. Heynens
Keyword(s):  
Human Blood ◽  
Blood Platelets ◽  
External Factors ◽  
Serotonin Uptake ◽  
Hypotonic Shock ◽  
Shock Response ◽  
Platelet Number ◽  
Ph Dependent ◽  
Human Blood Platelets

SummaryThe present paper is part of a study on the evaluation of the active serotonin uptake and the response to hypotonic shock as in vitro viability tests for human blood platelets.The extraction and fluorometric assay of serotonin in plasma and in platelets were further automated by the introduction of a dialyzer in the earlier described system. Furthermore, the influences of some external factors on both tests were investigated.The serotonin uptake as well as the hypotonic shock response were pH dependent and especially the latter was rather sensitive to small pH changes. Changes in the Na+ : K+ ratio were also found to influence both criteria significantly.The serotonin uptake appeared to be linearly dependent on the platelet number while, within a range of 200 X 103 - 450 X 103 platelets per fxl, the slope of the hypotonic shock response was the same.

scholarly journals Incorporation and turnover of eicosapentaenoic and docosahexaenoic acids in human blood platelets in vitro

1992 ◽  
Vol 281 (2) ◽  
pp. 309-316 ◽  
Author(s):  
M Croset ◽  
Y Bayon ◽  
M Lagarde
Keyword(s):  
Fatty Acids ◽  
Human Blood ◽  
Molecular Species ◽  
Blood Platelets ◽  
Blood Platelet ◽  
Total Radioactivity ◽  
Significant Release ◽  
Subsequent Activation ◽  
Human Blood Platelets

Mass changes in the incorporation of linoleic (C18:2), eicosapentaenoic (C20:5) and docosahexaenoic (C22:6) acids in human blood platelet phospholipids were induced by incubating the cells and these fatty acids complexed to albumin. The remodelling of [14C]C18:2, [14C]C20:5 and [14C]C22:6 in classes, subclasses and molecular species of platelet phospholipids was studied in resting and thrombin-stimulated cells. More than 85% of the incorporation was located in phospholipids, representing 5-fold and 2.5-fold increases in the phospholipid C20:5 and C22:6 endogenous content respectively. Thrombin stimulation induced a 30% degradation of 1-acyl-2-C20:5-glycerophosphocholine (GPC) and 1-acyl-2-C22:6-GPC, but did not induce significant release of C18:2 from 1-acyl-2-C18:2-GPC. There was no change in the [14C]fatty acid composition of 1-alkyl-2-acyl-GPC. Thrombin-dependent increases in 1-alkenyl-2-C20:5-glycerophosphoethanolamine (GPE) and 1-alkenyl-2-C22:6-GPE of 2.1-fold and 2.5-fold respectively accounted for the rise in GPE radioactivity and partly compensated for the loss of these fatty acids from 1,2-diacyl-GPC: transfer to 1-alkenyl-2-acyl-GPE was 0.4 and 1.5 nmol/10(9) platelets for C20:5 and C22:6 respectively. [14C]C20:5 and [14C]C22:6 were incorporated into six different species of 1,2-diacyl-GPC, with acylation in the major endogenous forms (C18:1 +C16:0 and C18:0 species) representing 76% and 66% respectively of the total radioactivity present in 1,2-diacyl-GPC. Stimulation by thrombin induced significant release of these fatty acids from the main molecular species of 1,2-diacyl-GPC, but significantly stimulated the synthesis of alkenyl forms of GPE containing C18:1/C22:6 +C16:0/C22:6, C18:0/C22:6 and C18:0/C20:5. C18:0/C18:2, the major endogenous C18:2 molecular species, represented only 10.5% of the incorporation; none of the [14C]C18:2 molecular species was a substrate for transfer towards 1-alkenyl-2-acyl-GPE. It is concluded that when C20:5 and C22:6, but not C18:2, are acylated in 1,2-diacyl-GPC, they participate in thrombin-dependent phospholipid remodelling, and might compete with the turnover and release of arachidonic acid from platelet phospholipids and the subsequent activation of the cells.

Adsorption of Carbon-14 Dextran to Human Blood Platelets and Red Blood Cells, in Vitro *

Vox Sanguinis ◽  
1957 ◽  
Vol 2 (2) ◽  
pp. 104-109
Author(s):  
S. Rothman ◽  
E. Adelson ◽  
A. Schwebel ◽  
R.D. Langdell ◽  
G. Fraction
Keyword(s):  
Red Blood Cells ◽  
Human Blood ◽  
Blood Cells ◽  
Blood Platelets ◽  
Carbon 14 ◽  
Human Blood Platelets
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