scholarly journals Immune complexes in sera and synovial fluids of patients with rheumatoid arthritis. Radioimmunoassay with monocylonal rheumatoid factor.

1975 ◽  
Vol 56 (2) ◽  
pp. 458-466 ◽  
Author(s):  
H S Luthra ◽  
F C McDuffie ◽  
G G Hunder ◽  
E A Samayoa
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Immune Complexes ◽  
Synovial Fluids

scholarly journals Immune Complexes in Rheumatoid Arthritis Sera and Synovial Fluids

1979 ◽  
Vol 22 (5) ◽  
pp. 440-448 ◽  
Author(s):  
James T. Halla ◽  
John E. Volanakis ◽  
Ralph E. Schrohenloher
Keyword(s):  
Rheumatoid Arthritis ◽  
Immune Complexes ◽  
Synovial Fluids

Capacity to solubilize immune complexes in sera and synovial fluids from patients with rheumatoid arthritis

1983 ◽  
Vol 26 (2) ◽  
pp. 156-164 ◽  
Author(s):  
E. Dayer ◽  
J. C. Gerster ◽  
M. T. Aguado ◽  
P. H. Lambert
Keyword(s):  
Rheumatoid Arthritis ◽  
Immune Complexes ◽  
Synovial Fluids

scholarly journals Demonstration of antibodies to collagen and of collagen-anticollagen immune complexes in rheumatoid arthritis synovial fluids

1976 ◽  
Vol 35 (5) ◽  
pp. 446-450 ◽  
Author(s):  
J. Menzel ◽  
C. Steffen ◽  
G. Kolarz ◽  
R. Eberl ◽  
O. Frank ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Immune Complexes ◽  
Synovial Fluids

IgM rheumatoid factor amplifies the inflammatory response of macrophages induced by the rheumatoid arthritis-specific immune complexes containing anticitrullinated protein antibodies

2014 ◽  
Vol 74 (7) ◽  
pp. 1425-1431 ◽  
Author(s):  
Lætitia Laurent ◽  
Florence Anquetil ◽  
Cyril Clavel ◽  
Ndiémé Ndongo-Thiam ◽  
Géraldine Offer ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Rheumatoid Factor ◽  
Immune Complexes ◽  
Cytokine Response ◽  
Igm Rheumatoid Factor ◽  
Tnf Α ◽  
Vitro Model ◽  
Synovial Membranes ◽  
Anticitrullinated Protein Antibodies

ObjectivesAnticitrullinated protein antibodies (ACPA) are specifically associated with rheumatoid arthritis (RA) and produced in inflamed synovial membranes where citrullinated fibrin, their antigenic target, is abundant. We showed that immune complexes containing IgG ACPA (ACPA-IC) induce FcγR-mediated tumour necrosis factor (TNF)-α secretion in macrophages. Since IgM rheumatoid factor (RF), an autoantibody directed to the Fc fragment of IgG, is also produced and concentrated in the rheumatoid synovial tissue, we evaluated its influence on macrophage stimulation by ACPA-IC.MethodsWith monocyte-derived macrophages from more than 40 healthy individuals and different human IgM cryoglobulins with RF activity, using a previously developed human in vitro model, we evaluated the effect of the incorporation of IgM RF into ACPA-IC.ResultsIgM RF induced an important amplification of the TNF-α secretion. This effect was not observed in monocytes and depended on an increase in the number of IgG-engaged FcγR. It extended to the secretion of interleukin (IL)-1β and IL-6, was paralleled by IL-8 secretion and was not associated with overwhelming secretion of IL-10 or IL-1Ra. Moreover, the RF-induced increased proinflammatory bioactivity of the cytokine response to ACPA-IC was confirmed by an enhanced, not entirely TNF-dependent, capacity of the secreted cytokine cocktail to prompt IL-6 secretion by RA synoviocytes.ConclusionsBy showing that it can greatly enhance the proinflammatory cytokine response induced in macrophages by the RA-specific ACPA-IC, these results highlight a previously undescribed, FcγR-dependent strong proinflammatory potential of IgM RF. They clarify the pathophysiological link between the presence of ACPA and IgM RF, and RA severity.

C1q-bearing immune complexes detected by a monoclonal antibody to human C1q in rheumatoid arthritis sera and synovial fluids

1991 ◽  
Vol 10 (6) ◽  
pp. 245-250 ◽  
Author(s):  
U. Antes ◽  
H. -P. Heinz ◽  
D. Schultz ◽  
D. Brackertz ◽  
M. Loos
Keyword(s):  
Rheumatoid Arthritis ◽  
Monoclonal Antibody ◽  
Immune Complexes ◽  
Synovial Fluids
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