scholarly journals The ionic control of 1,25-dihydroxyvitamin D3 synthesis in isolated chick renal mitochondria. The role of calcium as influenced by inorganic phosphate and hydrogen-ion.

1975 ◽  
Vol 55 (2) ◽  
pp. 299-304 ◽  
Author(s):  
D D Bikle ◽  
E W Murphy ◽  
H Rasmussen
Keyword(s):  
Inorganic Phosphate ◽  
Hydrogen Ion ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3

The role of monocytes in the suppression of PHA-induced proliferation and IL 2 production of human mononuclear cells by 1,25-dihydroxyvitamin D3

1985 ◽  
Vol 11 (5-6) ◽  
pp. 331-336 ◽  
Author(s):  
Sumiko Iho ◽  
Fumiaki Kura ◽  
Hiroyuki Sugiyama ◽  
Takayuki Takahashi ◽  
Takashi Hoshino
Keyword(s):  
Mononuclear Cells ◽  
Dihydroxyvitamin D3 ◽  
1,25 Dihydroxyvitamin D3 ◽  
Human Mononuclear Cells

scholarly journals Paracrine interactions between mesenchymal stem cells and macrophages are regulated by 1,25-dihydroxyvitamin D3

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Laura Saldaña ◽  
Gema Vallés ◽  
Fátima Bensiamar ◽  
Francisco José Mancebo ◽  
Eduardo García-Rey ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Three Dimensional ◽  
Soluble Factors ◽  
Dihydroxyvitamin D3 ◽  
Inflammatory Cytokine Production ◽  
1,25 Dihydroxyvitamin D3 ◽  
Important Regulator ◽  
Matrix Maturation

Abstract Mesenchymal stem cells (MSC) modulate the macrophage-mediated inflammatory response through the secretion of soluble factors. In addition to its classical effects on calcium homeostasis, 1,25-dihydroxyvitamin D3 (1,25D3) has emerged as an important regulator of the immune system. The present study investigates whether 1,25D3 modulates the paracrine interactions between MSC and macrophages. 1,25D3 stimulated MSC to produce PGE2 and VEGF and regulated the interplay between macrophages and MSC toward reduced pro-inflammatory cytokine production. Conditioned media (CM) from co-cultures of macrophages and MSC impaired MSC osteogenesis. However, MSC cultured in CM from 1,25D3-treated co-cultures showed increased matrix maturation and mineralization. Co-culturing MSC with macrophages prevented the 1,25D3-induced increase in RANKL levels, which correlated with up-regulation of OPG secretion. MSC seeding in three-dimensional (3D) substrates potentiated their immunomodulatory effects on macrophages. Exposure of 3D co-cultures to 1,25D3 further reduced the levels of soluble factors related to inflammation and chemotaxis. As a consequence of 1,25D3 treatment, the recruitment of monocytes toward CM of 3D co-cultures decreased, while the osteogenic maturation of MSC increased. These data add new insights into the pleiotropic effects of 1,25D3 on the crosstalk between MSC and macrophages and highlight the role of the hormone in bone regeneration.

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