scholarly journals On the in vivo action of erythropoietin: a quantitative analysis

1972 ◽  
Vol 51 (5) ◽  
pp. 1179-1185 ◽  
Author(s):  
Thalia Papayannopoulou ◽  
Clement A. Finch
Keyword(s):  
Quantitative Analysis

Solving the mechanisms responsible for organelle behavior in vivo by same-cell correlative light and electron microscopy

1992 ◽  
Vol 50 (1) ◽  
pp. 14-15
Author(s):  
Conly L. Rieder
Keyword(s):  
Electron Microscopy ◽  
Quantitative Analysis ◽  
Light Microscopy ◽  
Living Cell ◽  
Light And Electron Microscopy ◽  
Time Behavior ◽  
Dynamic Interactions ◽  
Positive Identification ◽  
Cellular Components

The behavior of many cellular components, and their dynamic interactions, can be characterized in the living cell with considerable spatial and temporal resolution by video-enhanced light microscopy (video-LM). Indeed, under the appropriate conditions video-LM can be used to determine the real-time behavior of organelles ≤ 25-nm in diameter (e.g., individual microtubules—see). However, when pushed to its limit the structures and components observed within the cell by video-LM cannot be resolved nor necessarily even identified, only detected. Positive identification and a quantitative analysis often requires the corresponding electron microcopy (EM).

Rabbit aortic medial thickness under relaxed and specified simulated in vivo conditions

1983 ◽  
Vol 245 (4) ◽  
pp. H623-H627
Author(s):  
G. Schneiderman ◽  
W. F. Pritchard ◽  
C. A. Ramirez ◽  
C. K. Colton ◽  
K. A. Smith ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Mean Arterial Pressure ◽  
Aortic Wall ◽  
Macromolecular Transport ◽  
Liquid Silicone ◽  
Normal Mean ◽  
Longitudinal Extension ◽  
Media Layer

A method is presented for measuring the thickness of the intima-media layer of the normal rabbit descending thoracic aortic wall under both relaxed (excised) and specified simulated in vivo conditions. The in vivo conditions were simulated by maintaining the aorta in situ at its normal longitudinal extension while perfusing its lumen at the normal mean arterial pressure with a mixture of liquid silicone polymer and a catalyst, thus providing physiological radial distension. After the rubber cured, both relaxed and extended-distended tissue segments were obtained from adjacent sites on the same aorta. These tissue segments were fixed and further processed for measurement of their medial thicknesses by light microscopy. This data was used to estimate the ratio of the medial thickness of the relaxed, excised aorta to that under in vivo conditions, 1.72 +/- 0.15. This information is required for quantitative analysis of data obtained from previous studies of in vivo macromolecular transport across the rabbit thoracic aortic wall.

scholarly journals Standardization of a method to study angiogenesis in a mouse model

2013 ◽  
Vol 85 (4) ◽  
pp. 1483-1487
Author(s):  
DAVID FEDER ◽  
FABIO F. PERRAZO ◽  
EDIMAR C. PEREIRA ◽  
SILVANA FORSAIT ◽  
CECILIA K.R. FEDER ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Animal Models ◽  
The Other ◽  
In Vivo Studies ◽  
Specific Method ◽  
Test Results ◽  
Hemoglobin Content ◽  
Physiological Conditions ◽  
Adult Organism

In the adult organism, angiogenesis is restricted to a few physiological conditions. On the other hand, uncontrolled angiogenesis have often been associated to angiogenesis-dependent pathologies. A variety of animal models have been described to provide more quantitative analysis of in vivo angiogenesis and to characterize pro- and antiangiogenic molecules. However, it is still necessary to establish a quantitative, reproducible and specific method for studies of angiogenesis factors and inhibitors. This work aimed to standardize a method for the study of angiogenesis and to investigate the effects of thalidomide on angiogenesis. Sponges of 0.5 x 0.5 x 0.5 cm were implanted in the back of mice groups, control and experimental (thalidomide 200 mg/K/day by gavage). After seven days, the sponges were removed. The dosage of hemoglobin in sponge and in circulation was performed and the ratio between the values was tested using nonparametric Mann-Whitney test. Results have shown that sponge-induced angiogenesis quantitated by ratio between hemoglobin content in serum and in sponge is a helpful model for in vivo studies on angiogenesis. Moreover, it was observed that sponge-induced angiogenesis can be suppressed by thalidomide, corroborating to the validity of the standardized method.

scholarly journals A Deep Learning Enhanced Novel Software Tool for Laryngeal Dynamics Analysis

2021 ◽  
pp. 1-15
Author(s):  
Andreas M. Kist ◽  
Pablo Gómez ◽  
Denis Dubrovskiy ◽  
Patrick Schlegel ◽  
Melda Kunduk ◽  
...  
Keyword(s):  
Neural Networks ◽  
Quantitative Analysis ◽  
High Speed ◽  
Voice Disorders ◽  
Vocal Folds ◽  
Video Data ◽  
Audio Data ◽  
Fully Automatic ◽  
Video And Audio

Purpose High-speed videoendoscopy (HSV) is an emerging, but barely used, endoscopy technique in the clinic to assess and diagnose voice disorders because of the lack of dedicated software to analyze the data. HSV allows to quantify the vocal fold oscillations by segmenting the glottal area. This challenging task has been tackled by various studies; however, the proposed approaches are mostly limited and not suitable for daily clinical routine. Method We developed a user-friendly software in C# that allows the editing, motion correction, segmentation, and quantitative analysis of HSV data. We further provide pretrained deep neural networks for fully automatic glottis segmentation. Results We freely provide our software Glottis Analysis Tools (GAT). Using GAT, we provide a general threshold-based region growing platform that enables the user to analyze data from various sources, such as in vivo recordings, ex vivo recordings, and high-speed footage of artificial vocal folds. Additionally, especially for in vivo recordings, we provide three robust neural networks at various speed and quality settings to allow a fully automatic glottis segmentation needed for application by untrained personnel. GAT further evaluates video and audio data in parallel and is able to extract various features from the video data, among others the glottal area waveform, that is, the changing glottal area over time. In total, GAT provides 79 unique quantitative analysis parameters for video- and audio-based signals. Many of these parameters have already been shown to reflect voice disorders, highlighting the clinical importance and usefulness of the GAT software. Conclusion GAT is a unique tool to process HSV and audio data to determine quantitative, clinically relevant parameters for research, diagnosis, and treatment of laryngeal disorders. Supplemental Material https://doi.org/10.23641/asha.14575533

scholarly journals Isolevuglandins covalently modify phosphatidylethanolamines in vivo: Detection and quantitative analysis of hydroxylactam adducts

2009 ◽  
Vol 47 (11) ◽  
pp. 1539-1552 ◽  
Author(s):  
Wei Li ◽  
James M. Laird ◽  
Liang Lu ◽  
Sanjoy Roychowdhury ◽  
Laura E. Nagy ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
In Vivo Detection

Quantitative Analysis of New Bone Formation in Various Bone Grafts by In - Vivo Tetracycline Labeling

1985 ◽  
Vol 20 (1) ◽  
pp. 1
Author(s):  
Sang Cheol Seong ◽  
Young Min Kim ◽  
Han Koo Lee ◽  
In Ho Choi ◽  
Moon Sang Chung ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Bone Formation ◽  
Bone Grafts ◽  
New Bone Formation ◽  
Tetracycline Labeling

Activity-Related Calcium Dynamics in Motoneurons of the Nucleus Hypoglossus From Mouse

1999 ◽  
Vol 82 (6) ◽  
pp. 2936-2946 ◽  
Author(s):  
Mario B. Lips ◽  
Bernhard U. Keller
Keyword(s):  
Quantitative Analysis ◽  
Action Potential ◽  
Calcium Binding ◽  
Calcium Influx ◽  
Binding Capacity ◽  
Calcium Transients ◽  
Calcium Dynamics ◽  
The Brain

A quantitative analysis of activity-related calcium dynamics was performed in motoneurons of the nucleus hypoglossus in the brain stem slice preparation from mouse by simultaneous patch-clamp and microfluorometric calcium measurements. Motoneurons were analyzed under in vitro conditions that kept them in a functionally intact state represented by rhythmic, inspiratory-related bursts of excitatory postsynaptic currents and associated action potential discharges. Bursts of electrical activity were paralleled by somatic calcium transients resulting from calcium influx through voltage-activated calcium channels, where each action potential accounted for a calcium-mediated charge influx around 2 pC into the somatic compartment. Under in vivo conditions, rhythmic-respiratory activity in young mice occurred at frequencies up to 5 Hz, demonstrating the necessity for rapid calcium elevation and recovery in respiratory-related neurons. The quantitative analysis of hypoglossal calcium homeostasis identified an average extrusion rate, but an exceptionally low endogenous calcium binding capacity as cellular parameters accounting for rapid calcium signaling. Our results suggest that dynamics of somatic calcium transients 1) define an upper limit for the maximum frequency of respiratory-related burst discharges and 2) represent a potentially dangerous determinant of intracellular calcium profiles during pathophysiological and/or excitotoxic conditions.

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