scholarly journals Bone Resorption in Tissue Culture. Factors Influencing the Response to Parathyroid Hormone *

1965 ◽  
Vol 44 (1) ◽  
pp. 103-116 ◽  
Author(s):  
Lawrence G. Raisz
Keyword(s):  
Tissue Culture ◽  
Parathyroid Hormone ◽  
Bone Resorption ◽  
Factors Influencing

Thyrocalcitonin Inhibition of Bone Resorption Induced by Parathyroid Hormone in Tissue Culture

Science ◽  
1966 ◽  
Vol 151 (3708) ◽  
pp. 330-331 ◽  
Author(s):  
M. A. Aliapoulios ◽  
P. Goldhaber ◽  
P. L. Munson
Keyword(s):  
Tissue Culture ◽  
Parathyroid Hormone ◽  
Bone Resorption

Factors influencing levels of bone resorption during denosumab dosing

2013 ◽  
Author(s):  
Richard Eastell ◽  
Ethel Siris ◽  
Christian Roux ◽  
Dennis M. Black ◽  
Nathalie Franchimont ◽  
...  
Keyword(s):  
Bone Resorption ◽  
Factors Influencing

Interaction between amrinone and parathyroid hormone on bone in culture

1982 ◽  
Vol 243 (6) ◽  
pp. E499-E504
Author(s):  
N. S. Krieger ◽  
P. H. Stern
Keyword(s):  
Parathyroid Hormone ◽  
Bone Resorption ◽  
Direct Interaction ◽  
Inhibitory Effects ◽  
Dihydroxyvitamin D3 ◽  
Basal Bone ◽  
Cardiotonic Agent ◽  
Neonatal Mouse Calvaria ◽  
Dose Dependent ◽  
Stimulation Of

The cardiotonic agent amrinone has been postulated to directly affect Na-Ca exchange. Because stimulated bone resorption has been proposed to require Na-Ca exchange, we examined the effects of amrinone on bone. Amrinone inhibited release of Ca from neonatal mouse calvaria in organ culture stimulated by parathyroid hormone (PTH), 1,25-dihydroxyvitamin d3, or prostaglandin E2. Inhibition was dose dependent and maximal at 2 X 10(-4) M. The effect of amrinone differed from the inhibitory effects of calcitonin, ouabain, or nigericin in that 1) 6-h exposure to amrinone alone prevented the effect of subsequently added PTH; 2) amrinone was only partially effective if added after resorption was initiated by 24-h treatment with PTH; 3) coincubation with amrinone and PTH during the first 48 h of culture allowed for a response to PTH after amrinone was removed; no such protection by a stimulator occurred with ouabain or nigericin. Also submaximal concentrations of amrinone plus calcitonin, ouabain, or nigericin gave greater than additive inhibition of Ca release. Amrinone had no effect on basal bone cAMP or on the acute stimulation of cAMP by PTH. The results suggest that amrinone could have a more direct interaction with the pathway involved in stimulated bone resorption than the other inhibitors.

scholarly journals SLPI is a critical mediator that controls PTH-induced bone formation

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Akito Morimoto ◽  
Junichi Kikuta ◽  
Keizo Nishikawa ◽  
Takao Sudo ◽  
Maki Uenaka ◽  
...  
Keyword(s):  
Parathyroid Hormone ◽  
Bone Resorption ◽  
Bone Formation ◽  
Protease Inhibitor ◽  
Bone Metabolism ◽  
Serine Protease ◽  
Serine Protease Inhibitor ◽  
Secretory Leukocyte Protease Inhibitor ◽  
Bone Imaging ◽  
Genetic Ablation

AbstractOsteoclastic bone resorption and osteoblastic bone formation/replenishment are closely coupled in bone metabolism. Anabolic parathyroid hormone (PTH), which is commonly used for treating osteoporosis, shifts the balance from osteoclastic to osteoblastic, although it is unclear how these cells are coordinately regulated by PTH. Here, we identify a serine protease inhibitor, secretory leukocyte protease inhibitor (SLPI), as a critical mediator that is involved in the PTH-mediated shift to the osteoblastic phase. Slpi is highly upregulated in osteoblasts by PTH, while genetic ablation of Slpi severely impairs PTH-induced bone formation. Slpi induction in osteoblasts enhances its differentiation, and increases osteoblast–osteoclast contact, thereby suppressing osteoclastic function. Intravital bone imaging reveals that the PTH-mediated association between osteoblasts and osteoclasts is disrupted in the absence of SLPI. Collectively, these results demonstrate that SLPI regulates the communication between osteoblasts and osteoclasts to promote PTH-induced bone anabolism.

Inhibition of Bone Resorption in Tissue Culture by Membrane-Stabilizing Drugs*

Endocrinology ◽  
1979 ◽  
Vol 104 (6) ◽  
pp. 1644-1648 ◽  
Author(s):  
JOHN W. DIETRICH ◽  
GREGORY R. MUNDY ◽  
LAWRENCE G. RAISZ
Keyword(s):  
Tissue Culture ◽  
Bone Resorption
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