scholarly journals MEASUREMENT OF SMALL QUANTITIES OF INSULIN-LIKE ACTIVITY WITH RAT ADIPOSE TISSUE. IV. SERUM INSULIN-LIKE ACTIVITY AND TUMOR INSULIN CONTENT IN PATIENTS WITH FUNCTIONING ISLET-CELL TUMORS*

1963 ◽  
Vol 42 (8) ◽  
pp. 1322-1329 ◽  
Author(s):  
Jurgen Steinke ◽  
J. Stuart Soeldner ◽  
Albert E. Renold
Keyword(s):  
Adipose Tissue ◽  
Islet Cell ◽  
Serum Insulin ◽  
Insulin Content ◽  
Islet Cell Tumors ◽  
Rat Adipose Tissue ◽  
Insulin Like Activity

scholarly journals MEASUREMENT OF SMALL QUANTITIES OF INSULIN-LIKE ACTIVITY WITH RAT ADIPOSE TISSUE. III. PERSISTENCE OF SERUM INSULIN-LIKE ACTIVITY AFTER PANCREATECTOMY*

1962 ◽  
Vol 41 (9) ◽  
pp. 1699-1707 ◽  
Author(s):  
Jurgen Steinke ◽  
Anna Sirek ◽  
Vilma Lauris ◽  
Francis D. W. Lukens ◽  
Albert E. Renold
Keyword(s):  
Adipose Tissue ◽  
Serum Insulin ◽  
Rat Adipose Tissue ◽  
Insulin Like Activity

PANCREATIC AND SERUM INSULIN IN THE NEW ZEALAND STRAIN OF OBESE MICE

1964 ◽  
Vol 28 (2) ◽  
pp. 163-172 ◽  
Author(s):  
J. G. T. SNEYD
Keyword(s):  
Adipose Tissue ◽  
New Zealand ◽  
Glucose Uptake ◽  
Serum Insulin ◽  
Porcine Insulin ◽  
Equivalent Amount ◽  
Obese Mice ◽  
Normal Controls ◽  
Stimulation Of ◽  
Insulin Like Activity

SUMMARY The insulin content of the pancreas and the insulin-like activity of the blood serum are considerably higher in mice of the New Zealand obese strain than in normal controls. The rate of glucose uptake by adipose tissue and hemi-diaphragms incubated in the absence of added insulin did not differ significantly in tissues from obese and normal mice and the stimulation of glucose uptake caused by addition of insulin was similar in tissues from the two strains. Insulin extracted from the pancreas of New Zealand obese mice stimulated glucose uptake by adipose tissue and muscle from obese mice to the same extent as an equivalent amount of porcine insulin.

Response of human adipose tissue to endogenous serum insulin-like activity in vitro

Metabolism ◽  
1967 ◽  
Vol 16 (1) ◽  
pp. 47-56 ◽  
Author(s):  
John A. Owen ◽  
Richard W. Lindsay ◽  
Joe H. Gaskin ◽  
Guy Hollifield
Keyword(s):  
Adipose Tissue ◽  
Serum Insulin ◽  
Human Adipose Tissue ◽  
Insulin Like Activity

scholarly journals MEASUREMENT OF SMALL QUANTITIES OF INSULIN-LIKE ACTIVITY USING RAT ADIPOSE TISSUE. I. A PROPOSED PROCEDURE*

1960 ◽  
Vol 39 (9) ◽  
pp. 1487-1498 ◽  
Author(s):  
Albert E. Renold ◽  
Donald B. Martin ◽  
Yves M. Dagenais ◽  
Jurgen Steinke ◽  
Rita J. Nickerson ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Rat Adipose Tissue ◽  
Insulin Like Activity

scholarly journals MEASUREMENT OF SMALL QUANTITIES OF INSULIN-LIKE ACTIVITY USING RAT ADIPOSE TISSUE. II. EVALUATION OF PERFORMANCE*

1960 ◽  
Vol 39 (9) ◽  
pp. 1499-1510 ◽  
Author(s):  
Mindel C. Sheps ◽  
Rita J. Nickerson ◽  
Yves M. Dagenais ◽  
Jurgen Steinke ◽  
Donald B. Martin ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Evaluation Of Performance ◽  
Rat Adipose Tissue ◽  
Insulin Like Activity

Assay of serum insulin and insulin-like activity on adipose tissue and muscle in vivo

Metabolism ◽  
1964 ◽  
Vol 13 (8) ◽  
pp. 769-774 ◽  
Author(s):  
George F. Cahill ◽  
Vilma Lauris ◽  
Stuart J. Soeldner ◽  
Dennis Slone ◽  
Jurgen Steinke
Keyword(s):  
Adipose Tissue ◽  
Serum Insulin ◽  
Insulin Like Activity

scholarly journals Immunocytochemistry of Normal Pancreatic Islets and Spontaneous Islet Cell Tumors in Dogs

1987 ◽  
Vol 24 (2) ◽  
pp. 170-179 ◽  
Author(s):  
K. L. Hawkins ◽  
B. A. Summers ◽  
F. P. Kuhajda ◽  
C. A. Smith
Keyword(s):  
Pancreatic Islets ◽  
Islet Cell ◽  
Serum Insulin ◽  
Cell Types ◽  
Neoplastic Cell ◽  
Islet Cell Tumors ◽  
Fixed Tissue ◽  
Formalin Fixed Tissue ◽  
Antibody Staining ◽  
Positive Immunoreactivity

Immunocytochemical studies of the distribution of glucagon, gastrin, insulin, and somatostatin in normal canine pancreatic islets and 20 canine islet cell tumors were done using the peroxidase-anti-peroxidase (PAP) technique. In the normal adult canine pancreas, islets typically consisted of clusters of 20–30 cells, but smaller foci and even individual cells were identified. Alpha cells (glucagon) were often peripherally located, beta cells (insulin) were centrally located and most numerous, and delta cells (somatostatin) were the least numerous and randomly located. Both juvenile and adult canine pancreases did not stain for gastrin. Of the 20 tumors examined, 18 had positive immunoreactivity for insulin, nine for glucagon, 14 for somatostatin, and one for gastrin. Two tumors were uninterpretable due to autolysis. Three tumors were pure insulinomas, but no pure somatostatinomas, glucagonomas, or gastrinomas were identified. Most tumors and metastases had mixed positive immunoreactivity; one neoplastic cell type predominated with lesser numbers of other cell types. Metastatic sites (liver and lymph node) stained for insulin and somatostatin, only. Foci of non-neoplastic islet cell tissue (nesidioblastosis), often located at the pancreatic-mesenteric junction, stained strongly positive for insulin, glucagon, and somatostatin but not for gastrin. The tumor staining pattern did not consistently correlate with tumor function, as determined by blood glucose and serum insulin assays. The PAP technique works well on paraffin-embedded, formalin-fixed tissue using rabbit or guinea pig antisera as the primary antibody. Staining occurred on sections of paraffin blocks stored for up to 7 years.

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