scholarly journals THE MEASUREMENT OF C-REACTIVE PROTEIN IN HUMAN SERA. COMPARISON OF THE CLINICAL TESTS ON THE BASIS OF A QUANTITATIVE METHOD

1951 ◽  
Vol 30 (6) ◽  
pp. 616-622 ◽  
Author(s):  
Harrison F. Wood ◽  
Maclyn McCarty
Keyword(s):  
Quantitative Method ◽  
Clinical Tests ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Human Sera

Rapid, semi-quantitative assay of C-reactive protein evaluated

1991 ◽  
Vol 37 (11) ◽  
pp. 1981-1982 ◽  
Author(s):  
H Vallance ◽  
G Lockitch
Keyword(s):  
Preterm Infants ◽  
Screening Test ◽  
Quantitative Method ◽  
Comparison Method ◽  
Quantitative Assay ◽  
Plasma Samples ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Nephelometric Method ◽  
Semiquantitative Method

Abstract We evaluated a new rapid semi-quantitative immunometric assay of C-reactive protein (CRP) as a screening test for sepsis by comparison with an automated nephelometric method. Plasma samples (n = 101) from preterm infants during the first week of life were saved for CRP analyses. We measured CRP by the Nycocard semiquantitative method and compared the results with those obtained with a Behring Nephelometer. A CRP value less than 10 mg/L was considered to be negative for infection. All CRP results read as less than 10 mg/L (negative) by the Nycocard method were also less than 10 mg/L by the comparison method, and all CRP values found to be greater than 20 mg/L (positive) by the Nycocard method were also positive by the comparison method. Results in the 10-20 mg/L range were considered equivocal. We conclude that the Nycocard CRP semi-quantitative method is a rapid and useful screening test for sepsis in preterm infants.

scholarly journals Expression of C-Reactive Protein in the Human Respiratory Tract

2001 ◽  
Vol 69 (3) ◽  
pp. 1747-1754 ◽  
Author(s):  
Jane M. Gould ◽  
Jeffrey N. Weiser
Keyword(s):  
Epithelial Cells ◽  
Respiratory Tract ◽  
Antimicrobial Effect ◽  
Acute Phase Reactant ◽  
C Reactive Protein ◽  
Human Respiratory Tract ◽  
Reactive Protein ◽  
Mucosal Surfaces ◽  
Human Sera ◽  
Respiratory Epithelial

ABSTRACT C-reactive protein (CRP) is a normal constituent of human sera synthesized by hepatocytes and induced by proinflammatory cytokines. The function of this acute-phase reactant includes activation of complement and enhancement of opsonophagocytosis. CRP binds to phosphorylcholine (ChoP), a constituent of eukaryotic membranes that is also found on the cell surface of major bacterial pathogens of the human respiratory tract, including Streptococcus pneumoniaeand Haemophilus influenzae. The presence of CRP on mucosal surfaces and role in innate immunity in the human respiratory tract where ChoP-containing organisms reside have not been previously studied. We have shown using a monoclonal antibody to CRP that CRP is present in inflamed (0.17 to 42 μg/ml) and uninflamed (<0.05 to 0.88 μg/ml) secretions from the human respiratory tract in sufficient quantities for an antimicrobial effect. In addition, the CRP gene was expressed in epithelial cells of the human respiratory tract using in situ hybridization on nasal polyps and reverse transcriptase PCR of pharyngeal cells in culture. The complement-dependent bactericidal activity of normal nasal airway surface fluid and sputum against ChoP-expressing H. influenzae was abolished when the secretions were pretreated to remove CRP. In summary, the results indicate that CRP is present in secretions of the human respiratory tract, that human respiratory epithelial cells are capable of CRP expression, and that this protein may contribute to bacterial clearance in the human respiratory tract.

scholarly journals A nationwide survey of the opinions of paediatricians with regards to the management of neonates born to women with group B Streptococcus in Japanese maternity homes

2019 ◽  
Author(s):  
kotomi yamaguch ◽  
Kazutomo Ohashi
Keyword(s):  
Management Strategies ◽  
Group B Streptococcus ◽  
Nationwide Survey ◽  
Clinical Tests ◽  
C Reactive Protein ◽  
Blood Cell Count ◽  
Reactive Protein ◽  
Intrapartum Antibiotic ◽  
Group B ◽  
Level Analysis

Abstract Objective: The Japanese Midwifery Association (JMA) guidelines allow midwives to handle group B Streptococcus (GBS)-positive women during pregnancy and labour at maternity homes. However, there are no guidelines to manage neonates born to GBS-positive women in Japan. We aimed to investigate the opinions of paediatricians regarding optimal management strategies for neonates born to GBS-positive women in maternity homes. A questionnaire was sent to paediatricians at 396 Japanese perinatal medical centres. We examined opinions regarding examinations and routine clinical tests for neonates born to GBS-positive women in maternity homes. Results: Of 235 paediatricians, only 11.2% considered that paediatric examinations were unnecessary for neonates born to GBS-positive women in maternity homes. Moreover, 20.5%, 13.2%, and 11.1% of paediatricians considered culture test of the nasal cavity, serum C-reactive protein level analysis, and blood cell count analysis, respectively, necessary for neonates born to GBS-positive pregnant women with intrapartum antibiotic prophylaxis (IAP); 36.3%, 56.2%, and 40.6% of paediatricians considered these tests necessary in cases without IAP. The JMA guidelines had low penetration rates among paediatricians in Japan. To manage neonates of GBS-positive women in maternity homes, midwives should engage with commissioned paediatricians in more detail and develop appropriate strategies to increase awareness and cooperation.

scholarly journals A nationwide survey of the opinions of paediatricians regarding the management of neonates born to women with group B Streptococcus in Japanese maternity homes

2019 ◽  
Author(s):  
kotomi yamaguchi ◽  
Kazutomo Ohashi
Keyword(s):  
Management Strategies ◽  
Group B Streptococcus ◽  
Nationwide Survey ◽  
Clinical Tests ◽  
C Reactive Protein ◽  
Blood Cell Count ◽  
Reactive Protein ◽  
Intrapartum Antibiotic ◽  
Group B ◽  
Level Analysis

Abstract Objective: The Japanese Midwifery Association (JMA) guidelines allow midwives to manage group B Streptococcus (GBS)-positive women during pregnancy and labour at maternity homes. However, no guidelines exist to manage neonates born to GBS-positive women in Japan. We aimed to investigate the opinions of paediatricians regarding optimal management strategies for neonates born to GBS-positive women in maternity homes. A questionnaire was sent to paediatricians at 396 Japanese perinatal medical centres. We examined opinions regarding examinations and routine clinical tests for neonates born to GBS-positive women in maternity homes. Results: Of 235 paediatricians, only 11.2% considered that paediatric examinations were unnecessary for neonates born to GBS-positive women in maternity homes. Moreover, 20.5%, 13.2%, and 11.1% of paediatricians considered culture test of the nasal cavity, serum C-reactive protein level analysis, and blood cell count analysis, respectively, necessary for neonates born to GBS-positive pregnant women with intrapartum antibiotic prophylaxis (IAP), whereas 36.3%, 56.2%, and 40.6% of paediatricians considered these tests necessary in cases without IAP. The JMA guidelines had low penetration rates among paediatricians in Japan. To manage neonates born to GBS-positive women in maternity homes, midwives should engage with commissioned paediatricians in more detail and develop appropriate strategies to increase awareness and cooperation.

scholarly journals A nationwide survey of the opinions of paediatricians regarding the management of neonates born to women with group B Streptococcus in Japanese maternity homes

2019 ◽  
Author(s):  
kotomi yamaguchi ◽  
Kazutomo Ohashi
Keyword(s):  
Management Strategies ◽  
Group B Streptococcus ◽  
Nationwide Survey ◽  
Clinical Tests ◽  
C Reactive Protein ◽  
Blood Cell Count ◽  
Reactive Protein ◽  
Intrapartum Antibiotic ◽  
Group B ◽  
Level Analysis

Abstract Objective: The Japanese Midwifery Association (JMA) guidelines allow midwives to manage group B Streptococcus (GBS)-positive women during pregnancy and labour at maternity homes. However, no guidelines exist to manage neonates born to GBS-positive women in Japan. We aimed to investigate the opinions of paediatricians regarding optimal management strategies for neonates born to GBS-positive women in maternity homes. A questionnaire was sent to paediatricians at 396 Japanese perinatal medical centres. We examined opinions regarding examinations and routine clinical tests for neonates born to GBS-positive women in maternity homes. Results: Of 235 paediatricians, only 11.2% considered that paediatric examinations were unnecessary for neonates born to GBS-positive women in maternity homes. Moreover, 20.5%, 13.2%, and 11.1% of paediatricians considered culture test of the nasal cavity, serum C-reactive protein level analysis, and blood cell count analysis, respectively, necessary for neonates born to GBS-positive pregnant women with intrapartum antibiotic prophylaxis (IAP), whereas 36.3%, 56.2%, and 40.6% of paediatricians considered these tests necessary in cases without IAP. The JMA guidelines had low penetration rates among paediatricians in Japan. To manage neonates born to GBS-positive women in maternity homes, midwives should engage with commissioned paediatricians in more detail and develop appropriate strategies to increase awareness and cooperation.

Label-free and quantitative analysis of C-reactive protein in human sera by tagged-internal standard assay on antibody arrays

2009 ◽  
Vol 24 (5) ◽  
pp. 1469-1473 ◽  
Author(s):  
Jae-Wan Jung ◽  
Se-Hui Jung ◽  
Je-Ok Yoo ◽  
In-Bum Suh ◽  
Young-Myeong Kim ◽  
...  
Keyword(s):  
Quantitative Analysis ◽  
Internal Standard ◽  
Label Free ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Standard Assay ◽  
Human Sera ◽  
Antibody Arrays
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