scholarly journals STUDIES IN METHIONINE METABOLISM. III. THE FATE OF INTRAVENOUSLY ADMINISTERED S35-LABELED-METHIONINE IN NORMAL ADULT MALES, IN PATIENTS WITH CHRONIC HEPATIC DISEASE, “IDIOPATHIC” HYPOPROTEINEMIA AND CUSHING'S SYNDROME 1

1950 ◽  
Vol 29 (2) ◽  
pp. 238-250 ◽  
Author(s):  
Laurance W. Kinsell ◽  
Sheldon Margen ◽  
Harold Tarver ◽  
Julie McB. Frantz ◽  
Erin K. Flanagan ◽  
...  
Keyword(s):  
Cushing’S Syndrome ◽  
Cushing's Syndrome ◽  
Hepatic Disease ◽  
Normal Adult ◽  
Methionine Metabolism ◽  
Adult Males ◽  
Chronic Hepatic Disease

HYDROXYSTEROID DEHYDROGENASES IN THE HUMAN ADRENAL CORTEX

1966 ◽  
Vol 34 (4) ◽  
pp. 439-NP ◽  
Author(s):  
K. C. CALMAN ◽  
A. H. BAILLIE ◽  
M. M. FERGUSON ◽  
D. McK. HART
Keyword(s):  
Dehydrogenase Activity ◽  
Cushing’S Syndrome ◽  
Adrenal Glands ◽  
Adrenal Adenoma ◽  
Hydroxysteroid Dehydrogenase ◽  
Cushing's Syndrome ◽  
Normal Adult ◽  
Zona Fasciculata ◽  
Adult Human ◽  
Hydroxysteroid Dehydrogenases

SUMMARY The histochemical utilization of 3α-, 3β-, 6β-, 11α-, 11β-, 12α-, 16α-, 16β-, 17α-, 17β-, 20α-, 21- and 24-hydroxysteroids by three normal adult human adrenal glands, two human foetal adrenal glands, three adrenals from patients with Cushing's syndrome and one adrenal adenoma are described. The normal adult human adrenal showed high 16β-hydroxysteroid dehydrogenase activity in the zona glomerulosa. Activity restricted to the outer part of the zona fasciculata was recorded with 3α-, 3β-, 6β-, 11β-, 16α-, 16β-, and 17β-hydroxysteroids. The zona reticularis utilized 3α-, 3β-, 11β-, 16β- and 17β-hydroxysteroids less well than the zona fasciculata. The adrenals of Cushing's syndrome showed activity only for 3β- and 16β-hydroxysteroid dehydrogenases; this activity was noted in all three zones. The activity pattern of the adrenal adenoma resembled that of the normal adult human adrenal except that greater activity for 16α-hydroxysteroid dehydrogenase was noted. The foetal part of the human foetal cortex was extremely active, showing 3α-, 3β-, 6β-, 11β-, 12α-, 16α-, 16β-, 17β-, 20β- and 21-hydroxysteroid dehydrogenase activity. The definitive cortex behaved similarly to the adult gland and possessed 3α-, 3β-, 11β-, 16β- and 17β-hydroxysteroid dehydrogenases; some evidence of zoning of the definitive cortex was seen with the 16β-hydroxysteroid. The relevance of these findings in the light of current knowledge of adrenal zonation is discussed.

Characterization of Tamm-Horsfall Urinary Glycoprotein

1973 ◽  
Vol 31 ◽  
pp. 420-421
Author(s):  
John P. Robinson ◽  
J. David Puett
Keyword(s):  
Electron Microscopy ◽  
Circular Dichroism ◽  
Secondary Structure ◽  
Quaternary Structure ◽  
Normal Adult ◽  
Morphological Properties ◽  
Adult Males ◽  
Circular Dichroïsm ◽  
Urinary Glycoprotein

Much work has been reported on the chemical, physical and morphological properties of urinary Tamm-Horsfall glycoprotein (THG). Although it was once reported that cystic fibrotic (CF) individuals had a defective THG, more recent data indicate that THG and CF-THG are similar if not identical.No studies on the conformational aspects have been reported on this glycoprotein using circular dichroism (CD). We examined the secondary structure of THG and derivatives under various conditions and have correlated these results with quaternary structure using electron microscopy.THG was prepared from normal adult males and CF-THG from a 16-year old CF female by the method of Tamm and Horsfall. CF female by the method of Tamm and Horsfall.

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