scholarly journals THE ORIGIN AND NATURE OF NORMAL HUMAN SYNOVIAL FLUID 12

1940 ◽  
Vol 19 (6) ◽  
pp. 795-799 ◽  
Author(s):  
Marian W. Ropes ◽  
Elsie C. Rossmeisl ◽  
Walter Bauer
Keyword(s):  
Synovial Fluid ◽  
Normal Human ◽  
Human Synovial Fluid

scholarly journals Isolation of human synovial-fluid hyaluronate by density-gradient ultracentrifugation and evaluation of its protein content

1972 ◽  
Vol 126 (5) ◽  
pp. 1073-1080 ◽  
Author(s):  
Irwin Scher ◽  
David Hamerman
Keyword(s):  
Synovial Fluid ◽  
Density Gradient ◽  
Density Gradient Ultracentrifugation ◽  
Proteolytic Digestion ◽  
Peptide Fragments ◽  
Guanidinium Chloride ◽  
Caesium Chloride ◽  
Normal Human ◽  
Protein Moiety ◽  
Human Synovial Fluid

1. A compound of hyaluronate and protein, called hyaluronate–protein was isolated from pooled human synovial fluids by caesium chloride density-gradient ultracentrifugation. 2. The isolated hyaluronate–protein was labelled with [125I]iodide and the following studies were done. (a) Ultracentrifugation in caesium chloride showed that the protein moiety (125I counts) and hyaluronate (hexuronate) sedimented together in the middle of the gradient. (b) The labelled hyaluronate–protein was treated with trypsin, and ultracentrifugation showed that peptide fragments (125I counts) were dispersed throughout the gradient, indicating proteolytic digestion. Hyaluronate sedimented in the middle of the gradient. (c) The labelled hyaluronate–protein was digested with streptococcal hyaluronidase, and ultracentrifugation showed that hyaluronate fragments were dispersed throughout the gradient, indicating digestion of the polysaccharide. The protein moiety, without attached hyaluronate, now sedimented at the top of the gradient. (d) Ultracentrifugation of labelled hyaluronate–protein in 4m-guanidinium chloride showed that protein and hyaluronate sedimented together. 3. These studies confirm that hyaluronate is combined with a small quantity of protein in normal human synovial fluid. A mild method for the rapid isolation of hyaluronate–protein in good yield is described.

Capillary electrophoresis of normal human synovial fluid: comparison with fluid from the disease states of osteoarthritis and rheumatoid arthritis

1994 ◽  
Vol 31 (9) ◽  
pp. 257 ◽  
Author(s):  
John M. Duffy ◽  
James Grimshaw ◽  
Aine Kane ◽  
Raymond A. B. Mollan ◽  
Peter L. Spedding ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Capillary Electrophoresis ◽  
Synovial Fluid ◽  
Disease States ◽  
Normal Human ◽  
Human Synovial Fluid

Normal human synovial fluid

1985 ◽  
Vol 67 (9) ◽  
pp. 1336-1339 ◽  
Author(s):  
M Baumgarten ◽  
R D Bloebaum ◽  
S D Ross ◽  
P Campbell ◽  
A Sarmiento
Keyword(s):  
Synovial Fluid ◽  
Normal Human ◽  
Human Synovial Fluid

The cytology of normal human synovial fluid

1940 ◽  
Vol 77 (2) ◽  
pp. 129-144 ◽  
Author(s):  
Howard C. Coggeshall ◽  
Charles F. Warren ◽  
Walter Bauer
Keyword(s):  
Synovial Fluid ◽  
Normal Human ◽  
Human Synovial Fluid

scholarly journals The LncRNA ZBED3-AS1 induces chondrogenesis of human synovial fluid mesenchymal stem cells

2017 ◽  
Vol 487 (2) ◽  
pp. 457-463 ◽  
Author(s):  
Farong Ou ◽  
Kai Su ◽  
Jiadong Sun ◽  
Wenting Liao ◽  
Yu Yao ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Synovial Fluid ◽  
Human Synovial Fluid

scholarly journals SAT0028 The importance of proper handling of human synovial fluid for arthritis research

2018 ◽  
Author(s):  
H. Brouwers ◽  
J.H. von Hegedus ◽  
R.E.M. Toes ◽  
T.W.J. Huizinga ◽  
M.A. Giera ◽  
...  
Keyword(s):  
Synovial Fluid ◽  
Human Synovial Fluid
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