scholarly journals Murine Model of Primary Acquired Ocular Toxoplasmosis: Fluorescein Angiography and Multiplex Immune Mediator Profiles in the Aqueous Humor

2021 ◽  
Vol 62 (3) ◽  
pp. 9
Author(s):  
Kexin Li ◽  
Xue Feng ◽  
Kenji Hikosaka ◽  
Kazumi Norose
Keyword(s):  
Fluorescein Angiography ◽  
Aqueous Humor ◽  
Murine Model ◽  
Ocular Toxoplasmosis ◽  
Immune Mediator

scholarly journals Immune Mediator Profile in Aqueous Humor Differs in Patients with Primary Acquired Ocular Toxoplasmosis and Recurrent Acute Ocular Toxoplasmosis

2019 ◽  
Vol 2019 ◽  
pp. 1-12 ◽  
Author(s):  
Claudia Thieme ◽  
Stephan Schlickeiser ◽  
Sylvia Metzner ◽  
Claudia Dames ◽  
Uwe Pleyer
Keyword(s):  
Inflammatory Response ◽  
Aqueous Humor ◽  
Clinical Characteristics ◽  
Multiplex Assay ◽  
Ocular Toxoplasmosis ◽  
Immune Mediator ◽  
Gm Csf ◽  
Immune Mediators ◽  
First Time ◽  
Type Response

Purpose. To compare the intraocular cytokine and chemokine profiles in patients with acute primary acquired ocular toxoplasmosis (pOT) or recurrent ocular toxoplasmosis (rOT) and to correlate them with their clinical characteristics.Methods. Aqueous humor samples were collected from 62 consecutive patients (21 pOT, 30 rOT, and 11 noninfected controls) and analyzed by multiplex assay. Correlations were assessed between cytokine/chemokine levels, type of inflammatory response (Th1, Th2, and Th17), and clinical characteristics. In all OT patients, the clinical diagnosis of either pOT or rOT was confirmed by positive intraocular Goldmann/Witmer-Desmonts coefficient. Correlations were assessed between a preselected panel of immune mediators and the clinical characteristics of OT.Results. In pOT patients, increased levels of IL-2, IFN-γ, TNF-α, IL-15, IL-4, IL-5, IL-9, IL-13, IL-17, IL-1Rα, IL-6, IL-1β, and chemokines MIP-1α, MIP-1β, IP-10, Eotaxin, IL-8, RANTES, PDGF-bb, GM-CSF, G-CSF, and MCP-1 were found in comparison to those in controls (p<0.05). Patients with rOT showed elevated levels of IL-2, IFN-γ, TNF-α, IL-15, IL-4, IL-5, IL-9, IL-17, IL-1Rα, IL-6, IL-1β, and chemokines MIP-1α, IP-10, Eotaxin, IL-8, RANTES, PDGF-bb, G-CSF, and MCP-1 compared to controls (p<0.05). In addition, IL-7 (p=0.028) differed between pOT and rOT; IL-9 (p=0.054) and IL-13 (p=0.051) showed a tendency of higher concentration in pOT than in rOT. A negative correlation was found between IL-7 (p=0.017) as well as IL-9 (p=0.008) and the number of recurrences. Cytokine ratios showed no difference between pOT and rOT, indicating a dominant Th1-type response in both infectious groups. Moreover, a positive correlation was detected between IL-7, VEGF, IL-13 and age at aqueous humor sampling (p<0.05).Conclusions. This study for the first time shows subtle differences between the intraocular cytokine profiles in patients with either acute pOT or rOT.

scholarly journals Pathological processes in aqueous humor due to iris atrophy predispose to early corneal graft failure in humans and mice

2020 ◽  
Vol 6 (20) ◽  
pp. eaaz5195 ◽  
Author(s):  
Takefumi Yamaguchi ◽  
Kazunari Higa ◽  
Yukari Yagi-Yaguchi ◽  
Koji Ueda ◽  
Hisashi Noma ◽  
...  
Keyword(s):  
Clinical Outcomes ◽  
Aqueous Humor ◽  
Murine Model ◽  
Graft Failure ◽  
Cellular Response ◽  
Energy Homeostasis ◽  
Corneal Transplantation ◽  
Full Spectrum ◽  
Therapeutic Modalities ◽  
Iris Atrophy

Corneal endothelial cell (CEnC) loss after corneal transplantation is the major cause of graft failure and remains a clinically relevant challenge to overcome. Accumulated knowledge derived from long-term clinical outcomes suggested that elevated protein levels in the aqueous humor are associated with CEnC loss. However, the full spectrum of driver proteins and molecular processes remains to be determined. Here, we defined the somatic microenvironmental landscape and cellular response across human aqueous humor in samples with poor corneal transplantation clinical outcomes using multiomics analyses and clarified specific driver alterations, including complement activation and disturbed energy homeostasis. These driver alterations were also confirmed in aqueous humor from a novel murine model that spontaneously develops iris atrophy, leading to CEnC loss. The application of the integrative multiomics performed in human samples to the novel murine model will help the development of therapeutic modalities for patients with CEnC loss after corneal transplantation.

DIAGNOSTIC VALUE OF POLYMERASE CHAIN REACTION IN BLOOD AND AQUEOUS HUMOR IN IMMUNOCOMPETENT PATIENTS WITH OCULAR TOXOPLASMOSIS

Retina ◽  
2000 ◽  
Vol 20 (6) ◽  
pp. 614 ◽  
Author(s):  
MARTA S. FIGUEROA ◽  
GERMAN BOU ◽  
PALOMA MARTI-BELDA ◽  
ROGELIO LOPEZ-VELEZ ◽  
ANTONIO GUERRERO
Keyword(s):  
Polymerase Chain Reaction ◽  
Aqueous Humor ◽  
Diagnostic Value ◽  
Ocular Toxoplasmosis ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Immunocompetent Patients

scholarly journals Early Aqueous Humor Analysis in Patients with Human Ocular Toxoplasmosis

2000 ◽  
Vol 38 (3) ◽  
pp. 996-1001 ◽  
Author(s):  
Justus G. Garweg ◽  
Patrick Jacquier ◽  
Matthias Boehnke
Keyword(s):  
Aqueous Humor ◽  
Laboratory Tests ◽  
Clinical Symptoms ◽  
Diagnostic Sensitivity ◽  
Ocular Toxoplasmosis ◽  
Enzyme Linked Immunosorbent Assay ◽  
Serum Samples ◽  
Diagnostic Significance ◽  
Paired Samples ◽  
Specific Igg

To evaluate the diagnostic sensitivity of a panel of laboratory tests for ocular toxoplasmosis performed at the time of presentation, paired samples of aqueous humor and serum were collected from 49 consecutive episodes of ocular toxoplasmosis with a clinical course of less than 3 weeks. Total immunoglobulin G (IgG) andToxoplasma gondii-specific IgG, IgM, and IgA were quantified by enzyme-linked immunosorbent assay. The avidity ofT. gondii-specific IgG was determined, and DNA extracted from aqueous humor was amplified for detection of a glycoprotein B gene sequence of T. gondii. The diagnosis was confirmed for 73% (36 of 49) of the patients; this rate rose to 79.5% if data from a later analysis of aqueous humor derived from five of the negative patients were included. The analysis of serum (detection of T. gondii-specific IgM and analysis of consecutive serum samples) alone did not contribute to the diagnosis. Calculation of local antibody production lacked diagnostic sensitivity when it was determined less than 3 weeks after the manifestation of clinical symptoms (28 of 49 patients [57%]), but this rose to 70% after an analysis of a second aqueous humor sample. The antibody avidity index attained diagnostic significance in only 8 of 43 instances (19%), andT. gondii DNA was amplified from no more than 6 of 39 (16%) aqueous humor samples. However, T. gondii-specific IgA was found within the aqueous humors of 11 of 43 patients (26%); measurement of the T. gondii-specific IgA level thus contributed substantially to the diagnostic sensitivity of the laboratory tests.

Clinicopathological features of a congenital murine model of ocular toxoplasmosis

1986 ◽  
Vol 224 (3) ◽  
pp. 256-264 ◽  
Author(s):  
Gordon N. Dutton ◽  
John Hay ◽  
David M. Hair ◽  
James Ralston
Keyword(s):  
Murine Model ◽  
Clinicopathological Features ◽  
Ocular Toxoplasmosis
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