scholarly journals Connexin 50-R205G Mutation Perturbs Lens Epithelial Cell Proliferation and Differentiation

2020 ◽  
Vol 61 (3) ◽  
pp. 25
Author(s):  
Nikki Tjahjono ◽  
Chun-hong Xia ◽  
Rachel Li ◽  
Sarah Chu ◽  
Jessica Wang ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Lens Epithelial Cell ◽  
Epithelial Cell Proliferation ◽  
Cell Proliferation And Differentiation ◽  
Proliferation And Differentiation ◽  
Connexin 50

scholarly journals Silencing hyperoxia-induced C/EBPα in neonatal mice improves lung architecture via enhanced proliferation of alveolar epithelial cells

2011 ◽  
Vol 301 (2) ◽  
pp. L187-L196 ◽  
Author(s):  
Guang Yang ◽  
Maurice D. Hinson ◽  
Jessica E. Bordner ◽  
Qing S. Lin ◽  
Amal P. Fernando ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Epithelial Cell ◽  
Type I ◽  
Epithelial Cell Proliferation ◽  
Newborn Mice ◽  
Alveolar Epithelial ◽  
Lung Morphology ◽  
Cell Proliferation And Differentiation ◽  
Hyperoxic Exposure ◽  
Proliferation And Differentiation

Postnatal lung development requires proliferation and differentiation of specific cell types at precise times to promote proper alveolar formation. Hyperoxic exposure can disrupt alveolarization by inhibiting cell growth; however, it is not fully understood how this is mediated. The transcription factor CCAAT/enhancer binding protein-α (C/EBPα) is highly expressed in the lung and plays a role in cell proliferation and differentiation in many tissues. After 72 h of hyperoxia, C/EBPα expression was significantly enhanced in the lungs of newborn mice. The increased C/EBPα protein was predominantly located in alveolar type II cells. Silencing of C/EBPα with a transpulmonary injection of C/EBPα small interfering RNA (siRNA) prior to hyperoxic exposure reduced expression of markers of type I cell and differentiation typically observed after hyperoxia but did not rescue the altered lung morphology at 72 h. Nevertheless, when C/EBPα hyperoxia-exposed siRNA-injected mice were allowed to recover for 2 wk in room air, lung epithelial cell proliferation was increased and lung morphology was restored compared with hyperoxia-exposed control siRNA-injected mice. These data suggest that C/EBPα is an important regulator of postnatal alveolar epithelial cell proliferation and differentiation during injury and repair.

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