scholarly journals Measuring the Contributions of Basal Laminar Deposit and Bruch's Membrane in Age-Related Macular Degeneration

2020 ◽  
Vol 61 (13) ◽  
pp. 19
Author(s):  
Amol A. Sura ◽  
Ling Chen ◽  
Jeffrey D. Messinger ◽  
Thomas A. Swain ◽  
Gerald McGwin ◽  
...  
Keyword(s):  
Macular Degeneration ◽  
Age Related Macular Degeneration ◽  
Bruch's Membrane ◽  
Bruch’S Membrane ◽  
Age Related

scholarly journals Towards an In Vitro Retinal Model to Study and Develop New Therapies for Age-Related Macular Degeneration

2021 ◽  
Vol 8 (2) ◽  
pp. 18
Author(s):  
Beatrice Belgio ◽  
Federica Boschetti ◽  
Sara Mantero
Keyword(s):  
Animal Models ◽  
Macular Degeneration ◽  
Tensile Testing ◽  
In Vitro Model ◽  
Age Related Macular Degeneration ◽  
Bruch's Membrane ◽  
Bruch’S Membrane ◽  
Age Related ◽  
Vitro Model

Age-related macular degeneration (AMD) is the leading cause of vision loss in the elderly worldwide. So far, the etiology and the progression of AMD are not well known. Animal models have been developed to study the mechanisms involved in AMD; however, according to the “Three Rs” principle, alternative methods have been investigated. Here we present a strategy to develop a “Three Rs” compliant retinal three-dimensional (3D) in vitro model, including a Bruch’s membrane model and retina pigment epithelium (RPE) layer. First, tensile testing was performed on porcine retina to set a reference for the in vitro model. The results of tensile testing showed a short linear region followed by a plastic region with peaks. Then, Bruch’s membrane (BrM) was fabricated via electrospinning by using Bombyx mori silk fibroin (BMSF) and polycaprolactone (PCL). The BrM properties and ARPE-19 cell responses to BrM substrates were investigated. The BrM model displayed a thickness of 44 µm, with a high porosity and an average fiber diameter of 1217 ± 101 nm. ARPE-19 cells adhered and spread on the BMSF/PCL electrospun membranes. In conclusion, we are developing a novel 3D in vitro retinal model towards the replacement of animal models in AMD studies.

Age-Dependent Changes in Heparan Sulfate in Human Bruch's Membrane: Implications for Age-Related Macular Degeneration

2014 ◽  
Vol 55 (8) ◽  
pp. 5370 ◽  
Author(s):  
Tiarnan D. L. Keenan ◽  
Claire E. Pickford ◽  
Rebecca J. Holley ◽  
Simon J. Clark ◽  
Wanchang Lin ◽  
...  
Keyword(s):  
Heparan Sulfate ◽  
Macular Degeneration ◽  
Age Related Macular Degeneration ◽  
Bruch's Membrane ◽  
Bruch’S Membrane ◽  
Age Related ◽  
Age Dependent

scholarly journals Integrin activation or alpha9 expression allows retinal pigmented epithelial cell adhesion on Bruch’s membrane in wet age-related macular degeneration

Brain ◽  
2010 ◽  
Vol 133 (2) ◽  
pp. 448-464 ◽  
Author(s):  
Fardad T. Afshari ◽  
Jessica C. Kwok ◽  
Melissa R. Andrews ◽  
Bas Blits ◽  
Keith R. Martin ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Epithelial Cell ◽  
Macular Degeneration ◽  
Age Related Macular Degeneration ◽  
Integrin Activation ◽  
Bruch's Membrane ◽  
Bruch’S Membrane ◽  
Age Related
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