scholarly journals Human Retinal Pigment Epithelium: In Vivo Cell Morphometry, Multispectral Autofluorescence, and Relationship to Cone Mosaic

2018 ◽  
Vol 59 (15) ◽  
pp. 5705 ◽  
Author(s):  
Charles E. Granger ◽  
Qiang Yang ◽  
Hongxin Song ◽  
Kenichi Saito ◽  
Koji Nozato ◽  
...  
Keyword(s):  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Human Retinal Pigment Epithelium ◽  
Cone Mosaic

The cytoskeletal elements of human retinal pigment epithelium: in vitro and in vivo

1988 ◽  
Vol 91 (2) ◽  
pp. 303-312
Author(s):  
N.M. McKechnie ◽  
M. Boulton ◽  
H.L. Robey ◽  
F.J. Savage ◽  
I. Grierson
Keyword(s):  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Cytokeratin 18 ◽  
Rpe Cells ◽  
Human Retinal Pigment Epithelium ◽  
Cytoskeletal Elements

The cytoskeletal elements of normal (in situ) and cultured human retinal pigment epithelium (RPE) were studied by a variety of immunocytochemical techniques. Primary antibodies to vimentin and cytokeratins were used. Positive immunoreactivity for vimentin was obtained with in situ and cultured material. The pattern of reactivity obtained with antisera and monoclonals to cytokeratins was more complex. Cytokeratin immunoreactivity could be demonstrated in situ and in cultured cells. The pattern of cytokeratin expression was similar to that of simple or glandular epithelia. A monoclonal antibody that specifically recognizes cytokeratin 18 identified a population of cultured RPE cells that had particularly well-defined filamentous networks within their cytoplasm. Freshly isolated RPE was cytokeratin 18 negative by immunofluorescence, but upon culture cytokeratin 18 positive cells were identifiable. Cytokeratin 18 positive cells were identified in all RPE cultures (other than early primaries), regardless of passage number, age or sex of the donor. In post-confluent cultures cytokeratin 18 cells were identified growing over cytokeratin 18 negative cells, suggesting an association of cytokeratin 18 immunoreactivity with cell proliferation. Immunofluorescence studies of retinal scar tissue from two individuals revealed the presence of numerous cytokeratin 18 positive cells. These findings indicate that RPE cells can be identified by their cytokeratin immunoreactivity and that the overt expression of cytokeratin 18 may be associated with proliferation of human RPE both in vitro and in vivo.

In vivo Quantitation of Autofluorescence in Human Retinal Pigment Epithelium

1989 ◽  
Vol 199 (2-3) ◽  
pp. 116-121 ◽  
Author(s):  
Keiko Kitagawa ◽  
Shozo Nishida ◽  
Yuichiro Ogura
Keyword(s):  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Human Retinal Pigment Epithelium

scholarly journals Human Retinal Pigment Epithelium Cells as Functional Models for the RPE In Vivo

2011 ◽  
Vol 52 (12) ◽  
pp. 8614 ◽  
Author(s):  
Zsolt Ablonczy ◽  
Mohammad Dahrouj ◽  
Peter H. Tang ◽  
Yueying Liu ◽  
Kumar Sambamurti ◽  
...  
Keyword(s):  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Human Retinal Pigment Epithelium ◽  
Functional Models ◽  
Retinal Pigment Epithelium Cells ◽  
Epithelium Cells

scholarly journals MCP-1–Activated Monocytes Induce Apoptosis in Human Retinal Pigment Epithelium

2011 ◽  
Vol 52 (8) ◽  
pp. 6026 ◽  
Author(s):  
Dongli Yang ◽  
Susan G. Elner ◽  
Xun Chen ◽  
Matthew G. Field ◽  
Howard R. Petty ◽  
...  
Keyword(s):  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Human Retinal Pigment Epithelium ◽  
Activated Monocytes

scholarly journals Determination of N-retinylidene-N-retinylethanolamine (A2E) levels in central and peripheral areas of human retinal pigment epithelium

2015 ◽  
Vol 14 (11) ◽  
pp. 1983-1990 ◽  
Author(s):  
Leopold Adler ◽  
Nicholas P. Boyer ◽  
David M. Anderson ◽  
Jeffrey M. Spraggins ◽  
Kevin L. Schey ◽  
...  
Keyword(s):  
Retinal Pigment Epithelium ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Central Area ◽  
Human Retinal Pigment Epithelium ◽  
Peripheral Areas

The levels of the bis-retinoid A2E cannot account for the high levels of lipofuscin fluorescence found in the central area of the human retinal pigment epithelium.

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