scholarly journals Sphingosine Kinase 2 Modulates Retinal Neovascularization in the Mouse Model of Oxygen-Induced Retinopathy

2018 ◽  
Vol 59 (2) ◽  
pp. 653 ◽  
Author(s):  
Jeanette Eresch ◽  
Martin Stumpf ◽  
Alexander Koch ◽  
Rajkumar Vutukuri ◽  
Nerea Ferreirós ◽  
...  
Keyword(s):  
Mouse Model ◽  
Sphingosine Kinase ◽  
Retinal Neovascularization ◽  
Sphingosine Kinase 2 ◽  
Oxygen Induced Retinopathy

scholarly journals Ephrin-A5 Is Involved in Retinal Neovascularization in a Mouse Model of Oxygen-Induced Retinopathy

2020 ◽  
Vol 2020 ◽  
pp. 1-10
Author(s):  
Wei Du ◽  
Lvzhen Huang ◽  
Xin Tang ◽  
Jiarui Li ◽  
Xiaoxin Li
Keyword(s):  
Mouse Model ◽  
Vision Loss ◽  
Biological Significance ◽  
Retinal Neovascularization ◽  
Pathological Feature ◽  
Downstream Signaling ◽  
Oxygen Induced Retinopathy ◽  
Severe Vision Loss ◽  
Retinal Angiogenesis ◽  
The Impact

Retinal neovascularization (RNV) is an important pathological feature of vitreoretinopathy that can lead to severe vision loss. The purpose of this study was to identify the role of ephrin-A5 (Efna5) in RNV and to explore its mechanism. The expression pattern and biological significance of Efna5 were investigated in a mouse model of oxygen-induced retinopathy (OIR). The expression of Efna5 and downstream signaling pathway members was determined by RT-PCR, immunofluorescence, immunohistochemistry, and western blot analyses. shRNA was used to knockdown Efna5 in the retina of the OIR mouse model. Retinal flat mounts were performed to evaluate the impact of Efna5 silencing on the RNV process. We found that the Efna5 was greatly upregulated in the retina of OIR mice. Elevated Efna5 mainly colocalized with the retinal vessels and endothelial cells. We then showed that knockdown of Efna5 in OIR mouse retinas using lentivirus-mediated shRNA markedly decreased the expression of Efna5 and reduced the retinal neovascularization and avascular retina area. We further showed hypoxia stimulation dramatically increased both total and phosphorylation levels of ERK1/2 and the phosphorylation levels of Akt in OIR mice. More importantly, knockdown of Efna5 could inhibit the p-Akt and p-ERK signaling pathways. Our results suggested that Efna5 may regulate the RNV. This study suggests that Efna5 was significantly upregulated in the retina of OIR mice and closely involved in the pathological retinal angiogenesis.

scholarly journals Sphingosine Kinase-2 Deficiency Ameliorates Kidney Fibrosis by Up-Regulating Smad7 in a Mouse Model of Unilateral Ureteral Obstruction

2017 ◽  
Vol 187 (11) ◽  
pp. 2413-2429 ◽  
Author(s):  
Stephanie Schwalm ◽  
Sandra Beyer ◽  
Helena Frey ◽  
Riad Haceni ◽  
Georgios Grammatikos ◽  
...  
Keyword(s):  
Mouse Model ◽  
Ureteral Obstruction ◽  
Sphingosine Kinase ◽  
Unilateral Ureteral Obstruction ◽  
Kidney Fibrosis ◽  
Sphingosine Kinase 2

scholarly journals Sulodexide inhibits retinal neovascularization in a mouse model of oxygen-induced retinopathy

BMB Reports ◽  
2014 ◽  
Vol 47 (11) ◽  
pp. 637-642 ◽  
Author(s):  
Hyoung Jo ◽  
Sang Hoon Jung ◽  
Jun Kang ◽  
Hye Bin Yim ◽  
Kui Dong Kang
Keyword(s):  
Mouse Model ◽  
Retinal Neovascularization ◽  
Oxygen Induced Retinopathy
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