scholarly journals Three-Dimensional Segmentation and Quantitative Measurement of the Aqueous Outflow System of Intact Mouse Eyes Based on Spectral Two-Photon Microscopy Techniques

2016 ◽  
Vol 57 (7) ◽  
pp. 3159 ◽  
Author(s):  
Xianzeng Zhang ◽  
Nenrong Liu ◽  
Peng Un Mak ◽  
Sio Hang Pun ◽  
Mang I. Vai ◽  
...  
Keyword(s):  
Quantitative Measurement ◽  
Three Dimensional ◽  
Intact Mouse ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Microscopy Techniques ◽  
Three Dimensional Segmentation ◽  
Aqueous Outflow ◽  
Outflow System

scholarly journals Microvascular Sprouting, Extension, and Creation of New Capillary Connections with Adaptation of the Neighboring Astrocytes in Adult Mouse Cortex under Chronic Hypoxia

2013 ◽  
Vol 34 (2) ◽  
pp. 325-331 ◽  
Author(s):  
Kazuto Masamoto ◽  
Hiroyuki Takuwa ◽  
Chie Seki ◽  
Junko Taniguchi ◽  
Yoshiaki Itoh ◽  
...  
Keyword(s):  
Adult Mouse ◽  
Fluorescent Protein ◽  
Vascular Endothelial Cells ◽  
Three Dimensional ◽  
Vascular Endothelial ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Mouse Cortex ◽  
Hypoxia Adaptation ◽  
Green Fluorescent

The present study aimed to determine the spatiotemporal dynamics of microvascular and astrocytic adaptation during hypoxia-induced cerebral angiogenesis. Adult C57BL/6J and Tie2-green fluorescent protein (GFP) mice with vascular endothelial cells expressing GFP were exposed to normobaric hypoxia for 3 weeks, whereas the three-dimensional microvessels and astrocytes were imaged repeatedly using two-photon microscopy. After 7 to14 days of hypoxia, a vessel sprout appeared from the capillaries with a bump-like head shape (mean diameter 14  μm), and stagnant blood cells were seen inside the sprout. However, no detectable changes in the astrocyte morphology were observed for this early phase of the hypoxia adaptation. More than 50% of the sprouts emerged from capillaries 60  μm away from the center penetrating arteries, which indicates that the capillary distant from the penetrating arteries is a favored site for sprouting. After 14 to 21 days of hypoxia, the sprouting vessels created a new connection with an existing capillary. In this phase, the shape of the new vessel and its blood flow were normalized, and the outside of the vessels were wrapped with numerous processes from the neighboring astrocytes. The findings indicate that hypoxia-induced cerebral angiogenesis provokes the adaptation of neighboring astrocytes, which may stabilize the blood–brain barrier in immature vessels.

Two-Photon Microscopy of Single Molecules

2000 ◽  
Vol 6 (S2) ◽  
pp. 802-803
Author(s):  
J. T. Fourkas ◽  
M. J. R. Previte ◽  
R. A. Farrer ◽  
C. Olson ◽  
L. A. Peyser
Keyword(s):  
Near Field ◽  
Single Molecules ◽  
Three Dimensional ◽  
Excitation Beam ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Higher Power ◽  
Excitation Techniques ◽  
Field Excitation ◽  
Heterogeneous Ensemble

The ability to observe the fluorescence arising from single molecules has revolutionized our ability to study the structure and dynamics of materials on a microscopic level and to probe the properties of individual members of a heterogeneous ensemble. A variety of near-field and far-field excitation techniques have been employed to study single molecules. Multiphoton excitation (MPE) techniques have a number of advantages that make them particularly attractive for singlemolecule detection. First, because the excitation and fluorescence wavelengths are significantly different from one another, Rayleigh and Raman scattering can easily be filtered out, leading to a low number of background counts. Second, because the probability for MPE depends on the excitation intensity to the second or higher power, the excitation is localized to the point in space where the excitation beam is most tightly focussed, thus providing three-dimensional resolution.

scholarly journals Study of the Development of the Mouse Thoracic Aorta Three-Dimensional Macromolecular Structure using Two-Photon Microscopy

2014 ◽  
Vol 63 (1) ◽  
pp. 8-21 ◽  
Author(s):  
Leah M. Zadrozny ◽  
Edward B. Neufeld ◽  
Bertrand M. Lucotte ◽  
Patricia S. Connelly ◽  
Zu-Xi Yu ◽  
...  
Keyword(s):  
Thoracic Aorta ◽  
Three Dimensional ◽  
Macromolecular Structure ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Recording Neural Activity in Unrestrained Animals with Three-Dimensional Tracking Two-Photon Microscopy

Cell Reports ◽  
2018 ◽  
Vol 25 (5) ◽  
pp. 1371-1383.e10 ◽  
Author(s):  
Doycho Karagyozov ◽  
Mirna Mihovilovic Skanata ◽  
Amanda Lesar ◽  
Marc Gershow
Keyword(s):  
Neural Activity ◽  
Three Dimensional ◽  
Two Photon Microscopy ◽  
Two Photon

scholarly journals Chronic imaging of mitochondria in the murine cerebral vasculature using in vivo two-photon microscopy

2020 ◽  
Vol 318 (6) ◽  
pp. H1379-H1386
Author(s):  
Ibolya Rutkai ◽  
Wesley R. Evans ◽  
Nikita Bess ◽  
Tomas Salter-Cid ◽  
Siniša Čikić ◽  
...  
Keyword(s):  
Cerebral Circulation ◽  
Three Dimensional ◽  
Cell Types ◽  
Two Photon Microscopy ◽  
Two Photon ◽  
Dimensional Reconstruction ◽  
Different Cell Types

We introduce an innovative in vivo approach to study mitochondria in the cerebral circulation in their physiological environment by demonstrating the feasibility of long-term imaging and three-dimensional reconstruction. We postulate that the appropriate combination of Cre/Lox system and two-photon microscopy will contribute to a better understanding of the role of mitochondria in not only endothelium but also the different cell types of the cerebral circulation.

Sign in / Sign up

Export Citation Format

Share Document